A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals
Middle East respiratory syndrome (mer) is a zoonosis caused by the mer deadly coronavirus (Mers-CoV) and pose a significant threat to public health worldwide. Therefore, serology rapid, sensitive, and specific for the detection of antibodies anti-Mers-CoV in both humans and animals is indispensable for the successful management of this disease. Here, we evaluated a wide range of novel luciferase immunosorbent assay (LISA) based on the nucleocapsid protein (NP) as well as fragments derived from the spike protein (S) including subunit 1 (S1), the N terminal domain (NTD), the domain of the receptor-binding (RBD) and subunit 2 (S2) from S to detect specific IgG Mers-CoV. fusion proteins, including nanoluciferase (NLuc) and various fragments derived from the NP or S protein of Mers-CoV, expressed in T cells are human embryonic kidney 293.
Lisas detectable anti-Mers-CoV IgG further developed using cell lysates expressing various fusion proteins. Panel samples of human or animal infected with Mers-CoV is used to analyze the sensitivity and specificity of various Lisas in reference to Mers-CoV RT-PCR, ELISA-based commercial S1 and particle pseudovirus neutralization test (ppNT). Our results indicate that S1, RBD-, and NP-Lisas more sensitive than the NTD- and S2-Lisas to detect anti-mer-CoV IgG.
Further, S1, RBD-, and NP-Lisas more sensitive (by at least 16-fold) compared with a commercially available ELISA S1. In addition, S1, RBD-, and NP-specifically recognized LISA anti-Mers-CoV IgG and do not cross-react with samples from other human CoV (OC43, 229E, HKU1, NL63) -infected patients. More importantly, this Lisas proven implementation and their reliability to detect anti-Mers-CoV IgG in the sample of camels, monkeys, and mice, among which the RBD-LISA exhibited excellent performance.
The results of this study indicate that Mers-CoV Novel RBD- and S1-Lisas is a very effective platform for rapid and sensitive detection of anti-mer-CoV IgG in human and animal samples. This test has the potential to be used as a serological test for the management and control of Mers-CoV infection.
A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals
Development of antigen-capture enzyme-linked immunosorbent assay and immunochromatographic strip based on monoclonal antibodies for the detection of the bird flu virus H6.
continuous surveillance has shown that the avian influenza virus subtype H6 (AIVs) which is prevalent in poultry and occasionally break barriers to infect the human species. Therefore it is necessary to build the specific, rapid and sensitive method to screen AIVs H6. In this study, a panel of monoclonal antibodies (mAbs) against hemagglutinin (HA) of the H6 AIV isolates produced.
Purified mAbs have high affinity and specificity to H6 AIVs. Antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) and immunochromatographic strip was developed based on two mAbs (1D7 and 1F12). Results of AC-ELISA shows high sensitivity with a limit of detection (LOD) of 3.9 ng / ml for HA protein and 0.5 H6 HAU (HA units) / 100 ml for life AIVs H6 subtypes. Average recovery AC-ELISA with allantoic fluid, respiratory specimens and cloacal swab was 91.907 ± 1.559%, 82.977% and 73.791 ± 1.497 ± 2.588%, respectively.
Description: Quantitative sandwich ELISA for measuring Human Creatinine (Cr) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Creatinine (Cr) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Creatinine (Cr) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Creatinine in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Creatinine in samples from serum, plasma, tissue homogenates or other biological fluids.
Human Urinary Creatinine(Urinary Creatinine) ELISA Kit
Description: A competitive ELISA for quantitative measurement of Rat Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This CD Creatinine ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Creatinine. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Description: A competitive ELISA for quantitative measurement of Human Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Description of target: Measuring serum creatinine is a simple test and it is the most commonly used indicator of renal function. In the United States, creatinine is typically reported in mg/dL, whereas, in Canada and a few European countries, umol/litre may be used. 1 mg/dL of creatinine is 88.4 umol/L. The typical human reference ranges for serum creatinine are 0.5 to 1.0 mg/dL (about 45-90 umol/L) for women and 0.7 to 1.2 mg/dL (60-110 umol/L) for men. While a baseline (medicine) serum creatinine of 2.0 mg/dL (150 umol/L) may indicate normal kidney function in a male body builder, a serum creatinine of 1.2 mg/dL (110 umol/L) can indicate significant renal disease in an elderly female. for male reference range are 60-120 umol/L and for female it is 50-110 umol/L. Creatinine is a break-down product of creatine phosphate in muscle, and is usually produced at a fairly constant rate by the body (depending on muscle mass).;Species reactivity: All;Application: ;Assay info: Assay Methodology: Quantitative Competitive ELISA;Sensitivity: 1.4umol/ml
Description: A competitive ELISA for quantitative measurement of Guinea pig Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Enzyme-linked immunosorbent assay kit for quantification of General Creatinine in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Urinary creatinine (UCR) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Urinary creatinine (UCR) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Urinary creatinine (UCR) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Cell Biolabs? Creatinine Assay Kit measures creatinine levels in urine. Samples are compared to a known concentration of creatinine standard within a 96-well microtiter plate format. Samples and standards are incubated for 30 minutes with a reaction reagent which changes color from yellow to bright orange upon reacting with creatinine, forming the creatinine-picrate complex. The plate is read with a standard 96-well spectrophotometric microplate reader at 490 nm. Higher OD values correlate with high creatinine concentrations. Sample creatinine concentrations are determined by comparison with the known creatinine standards.
Description: For quantitative determination of creatinine and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range: 4.8 to 500 µM or 0.054-5.7 mg/dL (colorimetric assay) and 0.25 to 100 µM or 0.0028-1.14 mg/dL (fluorimetric assay) for a 60 min reaction. It is 3- and 53-fold more sensitive than the traditional Jaffe method (e.g. DICT-500), especially useful for small samples or where high sensitivity is required. Convenient. The procedure involves adding a single working reagent and reading after 60 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day. Method: OD570nm; FL530/585nm. Samples: Urine, serum, plasma, and other biological preparations. Species: All. Procedure: 60 min. Size: 100 tests. Detection Limit: Colorimetric assay: 4.8 µM or 0.054 mg/dL; Fluorimetric assay: 0.25 µM or 0.0028 mg/dL.
Description: For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism. Key Features: Sensitive and accurate. Use 30 µL samples. Detection limit 0.10 mg/dL (8 µM) creatinine in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples. Method: OD510nm. Samples: Urine, serum, plasma and biological preparations. Species: All. Procedure: Assay takes 20 min. Kit size: 500 tests. Detection limit: 0.1 mg/dL (8 µM).
Serum Creatinine ELISA kit (colorimetric, all species), 96 tests, quantitative
Description: For quantitative determination of urinary protein and creatinine concentrations (protein/creatinine ratio, UPCR). Key Features: Sensitive and accurate. Use 20 µL samples. Linear detection range in 96-well plate: 1 - 20 mg/dL Protein and 1 - 150 mg/dL Creatinine. Fast and convenient. No sample pre-treatment is needed. Simple 10- minute "add-incubate-read" procedure. High-throughput adaptable. The procedure can be readily automated for processing thousands of samples per day. Method: OD600 and OD530nm. Samples: Urine samples. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 1 mg/dL Protein and 1 mg/dL Creatinine.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Creatine Kinase, Muscle (CKM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Creatine Kinase, Muscle (CKM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: Quantitativecompetitive ELISA kit for measuring Canine Creatine Kinase MB isoenzyme (CK-MB) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativecompetitive ELISA kit for measuring Canine Creatine Kinase MB isoenzyme (CK-MB) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Canine C-Reactive Protein, CRP in samples from serum, plasma, cell culture supernates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Canine C-Reactive Protein, CRP in samples from serum, plasma, cell culture supernates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
The coefficient of intra and inter-assay variation of less than 10%. LOD immunochromatographic strip is 1 HAU when evaluated by the naked eye, and the detection time of less than 10 minutes without any equipment. Storage at room temperature or 4 ° C for 30 days or 60 days had no effect on the sensitivity and specificity of the strip. Thus, the AC-ELISA and immunochromatographic strip described here could be a secondary method for diagnosing infection AIV H6 with high specificity, sensitivity, and stability.
