A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals

A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals

Middle East respiratory syndrome (mer) is a zoonosis caused by the mer deadly coronavirus (Mers-CoV) and pose a significant threat to public health worldwide. Therefore, serology rapid, sensitive, and specific for the detection of antibodies anti-Mers-CoV in both humans and animals is indispensable for the successful management of this disease. Here, we evaluated a wide range of novel luciferase immunosorbent assay (LISA) based on the nucleocapsid protein (NP) as well as fragments derived from the spike protein (S) including subunit 1 (S1), the N terminal domain (NTD), the domain of the receptor-binding (RBD) and subunit 2 (S2) from S to detect specific IgG Mers-CoV. fusion proteins, including nanoluciferase (NLuc) and various fragments derived from the NP or S protein of Mers-CoV, expressed in T cells are human embryonic kidney 293.

Lisas detectable anti-Mers-CoV IgG further developed using cell lysates expressing various fusion proteins. Panel samples of human or animal infected with Mers-CoV is used to analyze the sensitivity and specificity of various Lisas in reference to Mers-CoV RT-PCR, ELISA-based commercial S1 and particle pseudovirus neutralization test (ppNT). Our results indicate that S1, RBD-, and NP-Lisas more sensitive than the NTD- and S2-Lisas to detect anti-mer-CoV IgG.

Further, S1, RBD-, and NP-Lisas more sensitive (by at least 16-fold) compared with a commercially available ELISA S1. In addition, S1, RBD-, and NP-specifically recognized LISA anti-Mers-CoV IgG and do not cross-react with samples from other human CoV (OC43, 229E, HKU1, NL63) -infected patients. More importantly, this Lisas proven implementation and their reliability to detect anti-Mers-CoV IgG in the sample of camels, monkeys, and mice, among which the RBD-LISA exhibited excellent performance.

The results of this study indicate that Mers-CoV Novel RBD- and S1-Lisas is a very effective platform for rapid and sensitive detection of anti-mer-CoV IgG in human and animal samples. This test has the potential to be used as a serological test for the management and control of Mers-CoV infection.

A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals
A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals

Development of antigen-capture enzyme-linked immunosorbent assay and immunochromatographic strip based on monoclonal antibodies for the detection of the bird flu virus H6.

continuous surveillance has shown that the avian influenza virus subtype H6 (AIVs) which is prevalent in poultry and occasionally break barriers to infect the human species. Therefore it is necessary to build the specific, rapid and sensitive method to screen AIVs H6. In this study, a panel of monoclonal antibodies (mAbs) against hemagglutinin (HA) of the H6 AIV isolates produced.

Purified mAbs have high affinity and specificity to H6 AIVs. Antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) and immunochromatographic strip was developed based on two mAbs (1D7 and 1F12). Results of AC-ELISA shows high sensitivity with a limit of detection (LOD) of 3.9 ng / ml for HA protein and 0.5 H6 HAU (HA units) / 100 ml for life AIVs H6 subtypes. Average recovery AC-ELISA with allantoic fluid, respiratory specimens and cloacal swab was 91.907 ± 1.559%, 82.977% and 73.791 ± 1.497 ± 2.588%, respectively.

Human Cr(Creatinine) ELISA Kit

EH4306 96T
EUR 476.25
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 0.75nmol/ml

Bovine Cr(Creatinine) ELISA Kit

EB0090 96T
EUR 567.6
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Cattle;Sensitivity: 0.75nmol/ml

General Creatinine, Cr ELISA KIT

ELI-07047Ge 96 Tests
EUR 886

Mouse Cr(Creatinine) ELISA Kit

EM0950 96T
EUR 524.1
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 0.75nmol/ml

Rat Cr(Creatinine) ELISA Kit

ER0864 96T
EUR 524.1
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Rattus;Sensitivity: 0.75nmol/ml

Human Creatinine(Cr)ELISA Kit

GA-E1106HM-48T 48T
EUR 289

Human Creatinine(Cr)ELISA Kit

GA-E1106HM-96T 96T
EUR 466

Rat Creatinine(Cr)ELISA Kit

GA-E0315RT-48T 48T
EUR 317

Rat Creatinine(Cr)ELISA Kit

GA-E0315RT-96T 96T
EUR 496

Human Creatinine(Cr)ELISA Kit

QY-E03067 96T
EUR 361

Rat Creatinine(Cr)ELISA Kit

QY-E11337 96T
EUR 361

Mouse Creatinine(Cr)ELISA Kit

QY-E20401 96T
EUR 361

ELISA kit for Human Creatinine (Cr)

KTE62273-48T 48T
EUR 354
Description: Quantitative sandwich ELISA for measuring Human Creatinine (Cr) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Creatinine (Cr)

KTE62273-5platesof96wells 5 plates of 96 wells
EUR 2252
Description: Quantitative sandwich ELISA for measuring Human Creatinine (Cr) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Creatinine (Cr)

KTE62273-96T 96T
EUR 572
Description: Quantitative sandwich ELISA for measuring Human Creatinine (Cr) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Mouse Creatinine (Cr) CLIA Kit

abx196864-96tests 96 tests
EUR 825

Rat Creatinine (Cr) CLIA Kit

abx196865-96tests 96 tests
EUR 825

Cr ELISA Kit| Rat Creatinine ELISA Kit

EF017649 96 Tests
EUR 689

Cr ELISA Kit| Bovine Creatinine ELISA Kit

EF011042 96 Tests
EUR 689

Canine CR ELISA Kit

ECC0392 96Tests
EUR 521

Canine Cr ELISA Kit

ECC0828 96Tests
EUR 521

Human Calretinin (CR) ELISA Kit

DLR-CR-Hu-48T 48T
EUR 479
Description: A sandwich quantitative ELISA assay kit for detection of Human Calretinin (CR) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Calretinin (CR) ELISA Kit

DLR-CR-Hu-96T 96T
EUR 621
Description: A sandwich quantitative ELISA assay kit for detection of Human Calretinin (CR) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Calretinin (CR) ELISA Kit

RDR-CR-Hu-48Tests 48 Tests
EUR 500

Human Calretinin (CR) ELISA Kit

RDR-CR-Hu-96Tests 96 Tests
EUR 692

Human Calretinin (CR) ELISA Kit

RD-CR-Hu-48Tests 48 Tests
EUR 478

Human Calretinin (CR) ELISA Kit

RD-CR-Hu-96Tests 96 Tests
EUR 662

Creatinine ELISA Kit| Mouse Creatinine ELISA Kit

EF013537 96 Tests
EUR 689

Creatinine ELISA Kit

DLR-Crtn-Ge-48T 48T
EUR 469
Description: A competitive inhibition quantitative ELISA assay kit for detection of Creatinine in samples from serum, plasma, tissue homogenates or other biological fluids.

Creatinine ELISA Kit

DLR-Crtn-Ge-96T 96T
EUR 608
Description: A competitive inhibition quantitative ELISA assay kit for detection of Creatinine in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Urinary Creatinine(Urinary Creatinine) ELISA Kit

QY-E05390 96T
EUR 361

Mouse Creatinine ELISA Kit

DEIABL-M4 96T
EUR 627
Description: This CD Creatinine ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Creatinine. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Creatinine (Human) ELISA Kit

E4368-100
EUR 756

Creatinine (Mouse) ELISA Kit

E4369-100
EUR 805

Creatinine (Rat) ELISA Kit

E4370-100
EUR 805

Creatinine ELISA Kit (OKEH02617)

OKEH02617 96 Wells
EUR 688
Description: Description of target: Measuring serum creatinine is a simple test and it is the most commonly used indicator of renal function. In the United States, creatinine is typically reported in mg/dL, whereas, in Canada and a few European countries, umol/litre may be used. 1 mg/dL of creatinine is 88.4 umol/L. The typical human reference ranges for serum creatinine are 0.5 to 1.0 mg/dL (about 45-90 umol/L) for women and 0.7 to 1.2 mg/dL (60-110 umol/L) for men. While a baseline (medicine) serum creatinine of 2.0 mg/dL (150 umol/L) may indicate normal kidney function in a male body builder, a serum creatinine of 1.2 mg/dL (110 umol/L) can indicate significant renal disease in an elderly female. for male reference range are 60-120 umol/L and for female it is 50-110 umol/L. Creatinine is a break-down product of creatine phosphate in muscle, and is usually produced at a fairly constant rate by the body (depending on muscle mass).;Species reactivity: All;Application: ;Assay info: Assay Methodology: Quantitative Competitive ELISA;Sensitivity: 1.4umol/ml

Human Creatinine ELISA kit

E01C0629-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Creatinine ELISA kit

E01C0629-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Creatinine ELISA kit

E01C0629-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Creatinine ELISA kit

E03C0629-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Creatinine ELISA kit

E03C0629-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Creatinine ELISA kit

E03C0629-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Creatinine ELISA kit

E02C0629-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Creatinine ELISA kit

E02C0629-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Creatinine ELISA kit

E02C0629-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

General Creatinine ELISA Kit

RDR-Crtn-Ge-48Tests 48 Tests
EUR 488

General Creatinine ELISA Kit

RDR-Crtn-Ge-96Tests 96 Tests
EUR 676

General Creatinine ELISA Kit

RD-Crtn-Ge-48Tests 48 Tests
EUR 467

General Creatinine ELISA Kit

RD-Crtn-Ge-96Tests 96 Tests
EUR 646

Rabbit Creatinine ELISA kit

E04C0629-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Creatinine ELISA kit

E04C0629-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Creatinine ELISA kit

E04C0629-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Creatinine ELISA kit

E06C0629-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Creatinine ELISA kit

E06C0629-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Goat Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Creatinine ELISA kit

E06C0629-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Creatinine ELISA kit

E09C0629-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Creatinine ELISA kit

E09C0629-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Creatinine ELISA kit

E09C0629-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Creatinine ELISA kit

E07C0629-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Creatinine ELISA kit

E07C0629-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Creatinine ELISA kit

E07C0629-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Creatinine ELISA kit

E08C0629-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Creatinine ELISA kit

E08C0629-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Creatinine ELISA kit

E08C0629-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Creatinine in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Creatinine

B1717-50 50 mg
EUR 128
Description: Creatinine is a break-down product of creatine phosphate in muscle, and is usually produced at a fairly constant rate by the body.

Creatinine

HY-B0504 500mg
EUR 108

Creatinine

GK8780-100G 100 g
EUR 126

Creatinine

GK8780-25G 25 g
EUR 62

Creatinine

CB0328 5g
EUR 56.96

Human CR ELISA Kit

EHC0392 96Tests
EUR 521

Human Cr ELISA Kit

EHC0828 96Tests
EUR 521

Goat CR ELISA Kit

EGTC0392 96Tests
EUR 521

Goat Cr ELISA Kit

EGTC0828 96Tests
EUR 521

Bovine CR ELISA Kit

EBC0392 96Tests
EUR 521

Bovine Cr ELISA Kit

EBC0828 96Tests
EUR 521

Chicken CR ELISA Kit

ECKC0392 96Tests
EUR 521

Anserini CR ELISA Kit

EAC0392 96Tests
EUR 521

Anserine Cr ELISA Kit

EAC0828 96Tests
EUR 521

Cr ELISA KIT|Human

EF007458 96 Tests
EUR 689

Mouse CR ELISA Kit

EMC0392 96Tests
EUR 521

Mouse Cr ELISA Kit

EMC0828 96Tests
EUR 521

Rat CR ELISA Kit

ERC0392 96Tests
EUR 521

Rat Cr ELISA Kit

ERC0828 96Tests
EUR 521

Sheep CR ELISA Kit

ESC0392 96Tests
EUR 521

Rabbit CR ELISA Kit

ERTC0392 96Tests
EUR 521

Rabbit Cr ELISA Kit

ERTC0828 96Tests
EUR 521

Monkey CR ELISA Kit

EMKC0392 96Tests
EUR 521

Porcine CR ELISA Kit

EPC0392 96Tests
EUR 521

Porcine Cr ELISA Kit

EPC0828 96Tests
EUR 521

Creatinine Assay Kit

55R-1467 100 assays
EUR 638
Description: Assay Kit for detection of Creatinine in the research laboratory

Creatinine Assay Kit

abx098422-Hitachi7020R150ml3R250ml1 Hitachi 7020; R1: 50ml×3 R2: 50ml×1
EUR 519

Creatinine Assay Kit

abx098422-Hitachi7060R190ml2R260ml1 Hitachi 7060; R1: 90ml×2 R2: 60ml×1
EUR 472

Creatinine Assay Kit

abx098422-Toshiba120R140ml3R240ml1 Toshiba 120; R1: 40ml×3 R2: 40ml×1
EUR 566

Creatinine Assay Kit

abx098422-Toshiba120R150ml3R250ml1 Toshiba 120; R1: 50ml×3 R2: 50ml×1
EUR 519

The coefficient of intra and inter-assay variation of less than 10%. LOD immunochromatographic strip is 1 HAU when evaluated by the naked eye, and the detection time of less than 10 minutes without any equipment. Storage at room temperature or 4 ° C for 30 days or 60 days had no effect on the sensitivity and specificity of the strip. Thus, the AC-ELISA and immunochromatographic strip described here could be a secondary method for diagnosing infection AIV H6 with high specificity, sensitivity, and stability.

Leave a Comment

Your email address will not be published. Required fields are marked *