BioMag Concanavalin A – from Canavalia ensiformis

Concanavalin A (Con A) is a extensively used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are sometimes discovered all through the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.

• Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
• Detect glycoconjugates in microscopy and circulation cytometry
• Stain glycoproteins in gels
• Withstands fixation and permeabilization
• Variety of 10 CF dyes from UV to near-infrared
• Superior CF dyes are good, photostable, and water-soluble

Lectins are furthermore versatile probes for detecting glycoconjugates in microscopy and circulation cytometric features and for gel staining of glycoproteins. In impartial and alkaline selections, Con A exists as a tetramer with a molecular weight of roughly 104 kDa. In acidic selections (pH beneath 5.0), Con A exists as a dimer. Con A is likely to be utilized to selectively stain the cell floor of dwell cells, and stand as a lot as fixation and permeabilization. When cells are mounted and permeabilized ahead of staining, fluorescent lectins stain each cell floor and organelles all through the secretory pathway.

Biochem/physiol Actions

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