Concanavalin A from Canavalia ensiformis (Jack bean) has been used for creating zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been furthermore used all through the preparation of protein loaded microgels to stop enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).
Packaging
1 g in poly bottle
25, 100, 250 mg in poly bottle
PRODUCT DESCRIPTION
Concanavalin A (Con A) is a extensively used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are sometimes discovered all through the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.
Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
Detect glycoconjugates in microscopy and circulation cytometry
Stain glycoproteins in gels
Withstands fixation and permeabilization
Variety of 10 CF dyes from UV to near-infrared
Superior CF dyes are good, photostable, and water-soluble
Lectins are furthermore versatile probes for detecting glycoconjugates in microscopy and circulation cytometric features and for gel staining of glycoproteins. In impartial and alkaline selections, Con A exists as a tetramer with a molecular weight of roughly 104 kDa. In acidic selections (pH beneath 5.0), Con A exists as a dimer. Con A is likely to be utilized to selectively stain the cell floor of dwell cells, and stand as a lot as fixation and permeabilization. When cells are mounted and permeabilized ahead of staining, fluorescent lectins stain each cell floor and organelles all through the secretory pathway.
BioMag Plus
Biochem/physiol Actions
Con A shouldn’t be blood group specific nonetheless has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for prepare. Con A dissociates into dimers at pH 5.6 or beneath. Between pH 5.eight and pH 7.0, Con A exists as a tetramer; above pH 7.Zero larger aggregates are long-established. Con A reveals mitogenic prepare which is dependent upon its diploma of aggregation. Succinylation leads to an brisk dimeric sort which stays a dimer above pH 5.6.
Preparation Uncover
Terribly purified by specific affinity chromatography.
Evaluation Uncover
Agglutination prepare: Decided from serial dilutions of a 1 mg/mL reply (this prepare is the underside conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Kind A Human Blood.
Concanavalin A (Con A) might be going most likely probably the most sometimes used lectins in cell biology. Our Alexa Fluor 488 conjugate of Con A reveals the acute, inexperienced fluorescence of the Alexa Fluor® 488 dye (absorption/emission maxima ~495/519 nm). Alexa Fluor 488 Con A selectively binds to a-mannopyranosyl and a-glucopyranosyl residues.
Uncover the Right Stain for Your Utility
Con A and fully totally different lectins are carbohydrate binding proteins that acknowledge specific sugar moieties on glycoproteins. The presence and distribution of those targets fluctuate between cell varieties and tissues. In consequence, fully totally different cell floor stains or fully totally different lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, might produce bigger floor staining and can also be additional acceptable in your cell sort. Lectin conjugates is likely to be utilized to selectively stain the cell floor of dwell cells, and stand as a lot as fixation and permeabilization.
