Identification of natural anti-TMPRSS2 potential products through homology modeling, virtual screening and molecular dynamics simulation studies

The latest outbreak of the severe acute respiratory syndrome Coronavirus-2 (SARS-COV-2) has led to Covid-19 pandemic. The absence of therapeutic drugs and vaccines causes severe loss and economic losses throughout the world. SARS-COV and SARS-COV-2 employ host Serentease TMPRSSS2 for priming spike protein for viruses into the host cell. The potential way to reduce the initial site SARS-COV-2 infection is possible to inhibit TMPRSS2 activities. In this study, the three-dimensional structure of TMPRSSS2 was produced by homology modeling and then validated with a number of parameters.

Virtual screening based structure from the Selleckchem database is carried out through ‘virtual work flow’ (VSW) to determine the potential for tmprss2 inhibitors such as tin. Camostat and Bromhexine are known as TMPRSS2 inhibiting drugs, therefore this is used as a control molecule throughout the study. Based on better dock scores, binding free energy and binding interactions compared to control molecules, six molecules (neohesperidin, myricitrin, quercitrine, naringin, iCariin, and ambroxol) are found promising to TMPRSSS2.

Analysis of binding interactions reveals a number of significant binding interactions with the binding place of amino residue TMPRSS2. The simulation study of the All-Atom Molecular Dynamics (MD) indicates that all molecules proposed maintain in receptors in dynamic countries. Binding energy is calculated from the MD simulation trajectory also supports the strong affinity of molecules against TMPRSS2. The proposed molecules belong to the bioflavonoid phytochemical class and reported to have antivirus activity, our research shows possible potential applications in Covid-19. Communicated by Ramaswamy H. Sarma.

Molecular characterization of Campylobacter spp. Restored from beef products, chicken, sheep and pork in retail in Australia

The Australian Campylobacteriosis level is one of the highest in developed countries, but only a limited job that has been done to characterize the Campylobacter spp. in Australian retail products. We did the whole seuensing genome (WGS) on 331 C. coli and 285 C. Jejuni from retail chicken meat, as well as beef, chicken, sheep and jeracles (organs). Campylobacter isolates are very diverse, with 113 types of order (STS) including 38 STS novels, which were identified from 616 isolates. Genome analysis shows a very low level (2.3-15.3%) resistance to aminoglycosides, beta-lactam, fluoroquinolone, macrolide and tetracycline antibiotics.

The majority (> 90%) of isolates (52/56) have a T86i mutation related to fluoroquinolone in the GYRA gene owned by ST860, ST2083 or ST7323. Devote 44 pig isolates very diverse, representing 33 STS (11 novels STS) and buried genes related to resistance to aminoglycosides, lincosamides and makrolides which are generally not found in isolates from other sources. The prevalence of very low multidrug resistant genotypes (<5%), but ten times higher in C. coli than C. Jejuni. This research highlights the Campylobacter spp. From retail products in Australia are very diverse genotypes and important differences in existing antimicrobial resistance between campylobacter species and animal sources.

Molecular identification and evaluation of wild yeast performance from various Ethiopian fermentation products

Various types of yeast and lactic acid bacteria dominate in spontaneous fermented products (food, beverage, and spices) which are usually consumed in Ethiopia. The purpose of this study is to identify efficient fermentation yeast from fermented food, fermented drinks, honey and molasses using the genotype method. Of the 70 samples tested, 180 different wild yeast isolates were restored.

A total of 23 isolates are chosen for genome analysis based on the basis of biomass results, fermentation capacity, and yeast performance. Analysis of nucleotide sequences of the internal transcript spacer-5.8s RDNA region revealed that the original yeast isolates have a close link with Saccharomyces cerevisiae, Candida Humilis, Kazachstania Botderi, Pichia Fermentans and Pichia Kudriavzevii with nucleotide similarities greater than 97%. This study shows a diversity of high indigenous wild yeats in strong fermented products and strains have higher biomass results, good gas production and extraordinary yeast capacity that shows the potential attached to the roasting industry.

Molecular targets of natural products for chondroprotection in damaging joint diseases

Osteoarthritis (OA) is the most common type of arthritis that occurs in age population. This affects all joints in the body and decreases articular cartilage and subchondral bones. Although OA pathophysiology becomes different, vulnerable resorption is still a symbol of osteoarthritis. Matrix Metalloproteinases (MMPS) is an important proteolytic enzyme that decreases cellular matrix protein (ECM) in the body. MMP contributes to the turnover of cartilage and damage; Their level has increased in the Joint network of OA patients. The application of condropotective drugs neutralizes MMP activities.

Agarose gel DNA extraction and purification kit
K1441-100	Biovision	each	EUR 510

HiPurA® PCR Product and Gel Purification
MB563-20PR	EWC Diagnostics	1 unit	EUR 54.58
Description: HiPurA® PCR Product and Gel Purification

HiPurA® PCR Product and Gel Purification
MB563-250PR	EWC Diagnostics	1 unit	EUR 406.05
Description: HiPurA® PCR Product and Gel Purification

HiPurA® PCR Product and Gel Purification
MB563-50PR	EWC Diagnostics	1 unit	EUR 109.02
Description: HiPurA® PCR Product and Gel Purification

96-Well Plate PCR Products Purification Kit
BS365	Bio Basic	5XPlates, 480prep	EUR 300.37

EZ-10 Spin Column PCR Products Purification Kit
BS363	Bio Basic	50Preps	EUR 91.97

EZ-10 Spin Column PCR Products Purification Kit
BS364	Bio Basic	100preps	EUR 114.82

EZ-10 Spin Column PCR Products Purification Kit
BS664	Bio Basic	250preps	EUR 199.9

Ni-NTA His-Tag Purification Agarose
HY-K0210	MedChemExpress	50 mL	EUR 782.4

GeneMATRIX AGAROSE - OUT DNA Purification Kit
E3540-01	EURx	50preps	EUR 59.95
Description: Universal Kit for purification of DNA from agarose gels

GeneMATRIX AGAROSE - OUT DNA Purification Kit
E3540-02	EURx	150preps	EUR 167.86
Description: Universal Kit for purification of DNA from agarose gels

Purification Media, BioSeparation Affinity, Protein G (Agarose)
MBS635368-10mL	MyBiosource	10mL	EUR 1475

Purification Media, BioSeparation Affinity, Protein G (Agarose)
MBS635368-1mL	MyBiosource	1mL	EUR 505

Purification Media, BioSeparation Affinity, Protein G (Agarose)
MBS635368-5mL	MyBiosource	5mL	EUR 910

Purification Media, BioSeparation Affinity, Protein G (Agarose)
MBS635368-5x10mL	MyBiosource	5x10mL	EUR 6490

HiPurA® PCR Product Purification Kit
MB512-20PR	EWC Diagnostics	1 unit	EUR 29.23
Description: HiPurA® PCR Product Purification Kit

HiPurA® PCR Product Purification Kit
MB512-250PR	EWC Diagnostics	1 unit	EUR 259.94
Description: HiPurA® PCR Product Purification Kit

HiPurA® PCR Product Purification Kit
MB512-50PR	EWC Diagnostics	1 unit	EUR 62.37
Description: HiPurA® PCR Product Purification Kit

PURY PCR & Gel Purification Kit
P2006-100	GenDepot	100 Prep	EUR 160

EcoPURE PCR/Gel Purification Kit
E5003	Ecotech Biotechnology	50 rxn	EUR 41.8
Description: EcoPURE PCR/Gel Purification Kit combines 2 kits in 1. It is designed for effective and fast purification of polymerase chain reaction (PCR) products. Using this kit, primer dimers, free nucleotides in the reaction, salts, and Taq polymerase can be easily removed. This kit is also suitable for purification of nucleic acids from reactions including restriction digestion, alkaline phosphatase treatment, or kinase reactions.

HiPer® PCR Product Purification Teaching
HTBM041-10PR	EWC Diagnostics	1 unit	EUR 19
Description: HiPer® PCR Product Purification Teaching

HiPurA® 96 PCR Product Purification Kit
MB519-1X96PR	EWC Diagnostics	1 unit	EUR 145.13
Description: HiPurA® 96 PCR Product Purification Kit

Purification Media, BioSeparation Affinity, Protein A Beads (Agarose)
MBS635362-10mL	MyBiosource	10mL	EUR 990

Purification Media, BioSeparation Affinity, Protein A Beads (Agarose)
MBS635362-1mL	MyBiosource	1mL	EUR 460

Purification Media, BioSeparation Affinity, Protein A Beads (Agarose)
MBS635362-5mL	MyBiosource	5mL	EUR 675

Purification Media, BioSeparation Affinity, Protein A Beads (Agarose)
MBS635362-5x10mL	MyBiosource	5x10mL	EUR 4305

AffiPure Ni-NTA Agarose Bead,For His-Tagged protein purification
N3505-005	GenDepot	5ml	EUR 98

AffiPure Ni-NTA Agarose Bead,For His-Tagged protein purification
N3505-010	GenDepot	10ml	EUR 215

AffiPure Ni-NTA Agarose Bead,For His-Tagged protein purification
N3505-025	GenDepot	25ml	EUR 340

AffiPure Ni-NTA Agarose Bead,For His-Tagged protein purification
N3505-050	GenDepot	50ml	EUR 660

AffiPure Ni-NTA Agarose Bead,For His-Tagged protein purification
N3505-100	GenDepot	100ml	EUR 1190

AffiPure Ni-NTA Agarose Bead,For His-Tagged protein purification
N3505-500	GenDepot	500ml	EUR 4800

Gel and PCR DNA Purification kit
K1455-250	Biovision	250 Preps	EUR 576

Gel and PCR DNA Purification kit
K1455-50	Biovision	50 Preps	EUR 247.2

Custom Affinity Purification of antibodies (Coupling of antigen to Agarose, Ab purification (10 ml), ELISA, and affinity column
AFFI-I	Alpha Diagnostics	1	EUR 927.6

GEL/PCR Purification Mini Kit (100prep)
APGCK_100	Alphagen Biotech	100 extractions	EUR 20

GEL/PCR Purification Mini Kit (300prep)
APGCK_300	Alphagen Biotech	300 extractions	EUR 50

GEL/PCR Purification Mini Kit (100prep)
FAGCK-001	Favorgen	100 preps	EUR 154.8

GEL/PCR Purification Mini Kit (300prep)
FAGCK-001-1	Favorgen	300 preps	EUR 206.4

GEL/PCR Purification Mini Kit (100prep)
FAGCK_001	Favorgen	1 kit	EUR 22.5

GEL/PCR Purification Mini Kit (300prep)
FAGCK_001-1	Favorgen	1 kit	EUR 78.5

Biospin Gel and PCR Purification Kit
TRI-C02M1A	TRI Biotech	100T	EUR 176.4

MicroElute GEL/PCR Purification Kit (50prep)
FAEPK-001	Favorgen	50 preps	EUR 157.2

MicroElute GEL/PCR Purification Kit (200prep)
FAEPK-001-1	Favorgen	200 preps	EUR 249.6

MicroElute GEL/PCR Purification Kit (300prep)
FAEPK-001-2	Favorgen	300 preps	EUR 274.8

Quick PCR Purification and Gel Extraction Kit
MBS7608271-INQUIRE	MyBiosource	INQUIRE	Ask for price

T-Pro Gel/PCR DNA Purification Maxi Kit (10)
RB94-NEL010	T-Pro Biotechnology	10preps/Kit	EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (100)
RB94-NES100	T-Pro Biotechnology	100preps/Kit	EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (250)
RB94-NES250	T-Pro Biotechnology	250preps/Kit	EUR 266.4

PURY Gel Purification Kit
P2005-100	GenDepot	100 Prep	EUR 149

EcoSpin Gel Purification Kit
EcoGE-50x	Ecotech Biotechnology	50 rxn	EUR 38.5
Description: EcoSpin Gel Purification Kit is designed for effective and fast purification of polymerase chain reaction (PCR) products. Using this kit, primer dimers, free nucleotides in the reaction, salts, and Taq polymerase can be easily and effectively removed.

Gel DNA Purification Kit
EP006-200T	ELK Biotech	200T	EUR 93.5

Gel DNA Purification Kit
EP006-50T	ELK Biotech	50T	EUR 29

PCR Purification Kit
45700	Norgen Biotek Corp	250 Preps	EUR 291

PCR Purification Kit
14400	Norgen Biotek Corp	50 Preps	EUR 71.4

PCR Purification Kit
abx098084-100l	Abbexa	100 µl	EUR 250

PCR Purification Kit
abx098084-1ml	Abbexa	1 ml	Ask for price

PCR Purification Kit
abx098084-200l	Abbexa	200 µl	EUR 337.5

PCR Purification Kit
20-abx098084	Abbexa	Ask for price Ask for price	50 rxns 200 rxns

PCR Purification Kit
MBS308017-250Preps	MyBiosource	250Preps	EUR 360

PCR Purification Kit
MBS308017-50Preps	MyBiosource	50Preps	EUR 190

PCR Purification Kit
MBS8808200-200Tests	MyBiosource	200Tests	EUR 265

PCR Purification Kit
MBS8808200-50Tests	MyBiosource	50Tests	EUR 180

GEL Purification Mini Kit (50prep)
FAGPK-001	Favorgen	50 preps	EUR 144

GEL Purification Mini Kit (200prep)
FAGPK-001-1	Favorgen	200 preps	EUR 202.8

GEL Purification Mini Kit (300prep)
FAGPK-001-2	Favorgen	300 preps	EUR 226.8

GEL Purification Mini Kit (50prep)
FAGPK_001	Favorgen	1 kit	EUR 16

GEL Purification Mini Kit (200prep)
FAGPK_001-1	Favorgen	1 kit	EUR 54

GEL Purification Mini Kit (300prep)
FAGPK_001-2	Favorgen	1 kit	EUR 69.5

illustra GFx PCR DNA and Gel Band Purification Kit 100 prep - EACH
28903470	Scientific Laboratory Supplies	EACH	EUR 275.4

MicroElute GEL/PCR Purification Kit (100prep) (blister packing for columns)
FAEPK_001-1B	Favorgen	1 kit	EUR 64.96

MicroElute GEL/PCR Purification Kit (50prep) (blister packing for columns)
FAEPK_001B	Favorgen	1 kit	EUR 33

MicroElute GEL Purification Kit (50prep)
FAMGK-001	Favorgen	50 preps	EUR 151.2

MicroElute GEL Purification Kit (200prep)
FAMGK-001-1	Favorgen	200 preps	EUR 224.4

PURY PCR Purification Kit
P2071-100	GenDepot	100 Prep	Ask for price

PURY PCR Purification Kit
P2004-100	GenDepot	100 Prep	EUR 149

HiPurA® Quick Gel Purification Kit
MB539-20PR	EWC Diagnostics	1 unit	EUR 42.46
Description: HiPurA® Quick Gel Purification Kit

HiPurA® Quick Gel Purification Kit
MB539-250PR	EWC Diagnostics	1 unit	EUR 316.36
Description: HiPurA® Quick Gel Purification Kit

HiPurA® Quick Gel Purification Kit
MB539-50PR	EWC Diagnostics	1 unit	EUR 84.8
Description: HiPurA® Quick Gel Purification Kit

PCR Circle Purification Kit
EP005-200T	ELK Biotech	200T	EUR 93.5

PCR Circle Purification Kit
EP005-50T	ELK Biotech	50T	EUR 29

EcoSpin PCR Purification Kit
EcoPP-50x	Ecotech Biotechnology	50 rxn	EUR 38.5
Description: EcoSpin PCR Purification Kit is designed for effective and fast purification of polymerase chain reaction (PCR) products. Using this kit, primer dimers, free nucleaotides in the reaction, salts, and Taq polymerase can be easily removed. This kit is also suitable for purification of nucleic acids from reactions including restriction digestion, alkaline phosphatase treatment, or kinase reactions.

Biospin PCR Purification Kit
TRI-C03M1	TRI Biotech	100T	EUR 141.12

Biospin PCR Purification Kit
TRI-C03S1	TRI Biotech	50T	EUR 95.76

MagaBio PCR Purification Kit
TRI-C42M1	TRI Biotech	100T	EUR 352.8

MagaBio PCR Purification Kit
TRI-C42S1	TRI Biotech	50T	EUR 189

MagaBio PCR Purification Kit
TRI-C42S1C	TRI Biotech	32T	EUR 161.28

MagaBio PCR Purification Kit
TRI-C42S1E	TRI Biotech	32T	EUR 176.4

MagaBio PCR Purification Kit
TRI-C42S1S	TRI Biotech	32T	EUR 403.2

96-well GEL Purification Kit (1 prep)
FAGKE-96001	Favorgen	1 prep	EUR 189.6

96-well GEL Purification Kit (2 prep)
FAGKE-96002	Favorgen	2 preps	EUR 255.6

96-well GEL Purification Kit (4 prep)
FAGKE-96004	Favorgen	4 preps	EUR 380.4

96-well GEL Purification Kit (1 prep)
FAGKE_96001	Favorgen	1 kit	EUR 45

96-well GEL Purification Kit (2 prep)
FAGKE_96002	Favorgen	1 kit	EUR 87.5

96-well GEL Purification Kit (4 prep)
FAGKE_96004	Favorgen	1 kit	EUR 168

Zenoquick PCR Purification Kit
Z3002-050	GenDepot	50 preps	EUR 262.8

Zenoquick PCR Purification Kit
Z3002-200	GenDepot	4x50 preps	EUR 757.2

PCR Purification - 96 Well Kit
24800	Norgen Biotek Corp	2 Plates	EUR 263.4

Genorise® Gel DNA Purification Mini Kit-PCR Clean Kit- 50
GR101080	Genorise Scientific	50	EUR 148

Genorise® Gel DNA Purification Mini Kit-PCR Clean Kit- 100
GR101080-100	Genorise Scientific	100	EUR 267

Genorise® Gel DNA Purification Mini Kit-PCR Clean Kit- 200
GR101081	Genorise Scientific	200	EUR 477

Genorise® Gel DNA Purification Mini Kit-PCR Clean Kit- 500
GR101081-500	Genorise Scientific	500	EUR 1074

PCR Purification Kit (100 preps)
9K-006-0003	Bio Basic	100preps	EUR 268.8

PCR Purification Kit (200 preps)
9K-006-0004	Bio Basic	200preps, 200prep	EUR 373.2

PCR Purification Kit - Column Kit
PP-201L	Jena Bioscience GmbH	250preparations	EUR 189.91

PCR Purification Kit - Column Kit
PP-201S	Jena Bioscience GmbH	50preparations	EUR 49.06

PCR DNA Extraction and Purification
EP10013	Neuromics	100 Tests	EUR 187.2

PCR DNA Extraction and Purification
MBS555986-100Tests	MyBiosource	100Tests	EUR 330

PCR DNA Extraction and Purification
MBS555986-5x100Tests	MyBiosource	5x100Tests	EUR 1495

PCR DNA extraction and purification kit
K1444-100	Biovision	each	EUR 405.6

GeneMAGNET PCR/ DNA CLEAN - UP Purification Kit
E3420-01	EURx	96preps	EUR 138.43

GeneMATRIX PCR/ DNA CLEAN - UP Purification Kit
E3520-01	EURx	50preps	EUR 50.14
Description: Kit for purification of PCR products / DNA after enzymatic reactions

GeneMATRIX PCR/ DNA CLEAN - UP Purification Kit
E3520-02	EURx	150preps	EUR 138.43
Description: Kit for purification of PCR products / DNA after enzymatic reactions

MicroElute GEL Purification Kit (100prep) (blister packing for columns)
FAMGK_001-1B	Favorgen	1 kit	EUR 56

MicroElute GEL Purification Kit (50prep) (blister packing for columns)
FAMGK_001B	Favorgen	1 kit	EUR 28

BioLit PCR and DNA Fragment Purification Kit (Research)
23512	Sisco Laboratories	50 Preps	EUR 94.08
Description: Part E

96-well GE/PCR Clean Up Purification Kit (1 plates)
FAPKE-96001	Favorgen	1 prep	EUR 175.2

96-well GE/PCR Clean Up Purification Kit (2 plates)
FAPKE-96002	Favorgen	2 preps	EUR 225.6

96-well GE/PCR Clean Up Purification Kit (4 plates)
FAPKE-96004	Favorgen	4 preps	EUR 328.8

96-well GE/PCR Clean Up Purification Kit (1 plates)
FAPKE_96001	Favorgen	1 kit	EUR 36

96-well GE/PCR Clean Up Purification Kit (2 plates)
FAPKE_96002	Favorgen	1 kit	EUR 68.5

96-well GE/PCR Clean Up Purification Kit (4 plates)
FAPKE_96004	Favorgen	1 kit	EUR 134.5

Bacterial DNA Purification Kit II
K1457-250	Biovision	250 Preps	EUR 697.2

Bacterial DNA Purification Kit II
K1457-50	Biovision	50 Preps	EUR 381.6

PCR Purification Solutions Kit (for 50x 96 well-plates)
9K-006-0005	Bio Basic	50, 100ug	EUR 541.8

PCR Purification Kit, Centrifugal Colμmn Method, 50 reactions/pk
208101	NEST Biotechnology	8 pcs/cs	EUR 22.55
Description: PCR Purification Kit, Centrifugal Colμmn Method, 50 reactions/pk

MagaBio Fecal pathogens DNA/RNA Purification Kit Purification Kit
TRI-C78L1E	TRI Biotech	96T	EUR 1058.4

MagaBio Fecal pathogens DNA/RNA Purification Kit Purification Kit
TRI-C78M1	TRI Biotech	100T	EUR 856.8

MagaBio Fecal pathogens DNA/RNA Purification Kit Purification Kit
TRI-C78S1	TRI Biotech	50T	EUR 453.6

MagaBio Fecal pathogens DNA/RNA Purification Kit Purification Kit
TRI-C78S1E	TRI Biotech	32T	EUR 378

Recombinant Protein A+G-Agarose suspension for Ig's purification
PRAG15-AS-1	Alpha Diagnostics	1 ml	EUR 242.4

Recombinant Protein A+G-Agarose suspension for Ig's purification
PRAG15-AS-5	Alpha Diagnostics	5 ml	EUR 781.2

MagaBio plus Virus DNA Purification Kit II
TRI-C50S1E	TRI Biotech	32T	EUR 241.92

MagaBio plus Total RNA Purification Kit II
TRI-C69L1E	TRI Biotech	96 T	EUR 1512

MagaBio plus Total RNA Purification Kit II
TRI-C69M1B	TRI Biotech	100T	EUR 1078.56

MagaBio plus Total RNA Purification Kit II
TRI-C69M1E	TRI Biotech	48 T	EUR 851.76

MagaBio plus Total RNA Purification Kit II
TRI-C69S1B	TRI Biotech	50T	EUR 579.6

MagaBio plus Total RNA Purification Kit II
TRI-C69S1C	TRI Biotech	32T	EUR 831.6

MagaBio plus Total RNA Purification Kit II
TRI-C69S1C-C	TRI Biotech	32T	EUR 504

MagaBio plus Total RNA Purification Kit II
TRI-C69S1E	TRI Biotech	32T	EUR 831.6

MagaBio plus Total RNA Purification Kit II
TRI-C69S1S	TRI Biotech	32T	EUR 929.88

MagaBio plus Virus RNA Purification Kit II
TRI-C87L1E	TRI Biotech	96T	EUR 1209.6

MagaBio plus Virus RNA Purification Kit II
TRI-C87L1E-T	TRI Biotech	96T	EUR 1209.6

MagaBio plus Virus RNA Purification Kit II
TRI-C87M1B	TRI Biotech	100T	EUR 869.4

MagaBio plus Virus RNA Purification Kit II
TRI-C87M1E	TRI Biotech	48T	EUR 650.16

MagaBio plus Virus RNA Purification Kit II
TRI-C87M1E-T	TRI Biotech	48T	EUR 650.16

MagaBio plus Virus RNA Purification Kit II
TRI-C87M1S	TRI Biotech	48T	EUR 687.96

MagaBio plus Virus RNA Purification Kit II
TRI-C87S1B	TRI Biotech	50T	EUR 453.6

MagaBio plus Virus RNA Purification Kit II
TRI-C87S1E	TRI Biotech	32T	EUR 403.2

MagaBio plus Virus RNA Purification Kit II
TRI-C87S1P	TRI Biotech	32T	EUR 430.92

Natural products originating from herbs and plants developed as traditional drugs have been considered, due to their potential biological effects. The therapeutic value of natural products in OA has increased in a reputation due to their clinical impact and unsignificant side effects. Some MMP inhibitors have been used as therapeutic drugs, for a long time. Recently, various types of compounds were reviewed for their biological activities. In this review, we summarize many natural products for the development of MMP inhibitors in rheumatic diseases and describe the main signal targets involved for the treatment of damaging joint diseases.