Primarily primarily probably the most usually used histological stain, hematoxylin is a pure compound extracted from a species of tree present in Mexico and the West Indies. The extracted compound is oxidized to provide hematein, the energetic staining ingredient of the hematoxylin stain.
The manufacturing of histological staining selections is an work. Just a few laboratories are able to making a stain of dependable vibrance. At Nationwide Diagnostics we have now now now gone one step additional.
Histo-Clear II
Low Hazard
Biodegradable
Lowered Citrus Odor
Histo-Clear II, like its predecessor Histo-Clear, is a safer histological clearing agent that results in the manufacturing of high-quality tissue slides. Histo-Clear II is unhazardous and completely biodegradable, thus lowering disposal prices. Histo-Clear II has a vastly diminished citrus odor as in contrast with utterly totally different citrus-based histological clearing brokers.
Uncover: Histo-Clear II is just not applicable with many artificial mounting media. Histo-Clear II is a long-standing favourite dry mounting procedures and aqueous mounting. Please affirm compatibility alongside collectively together with your mounting media before utilizing Histo-Clear II with in wet-mounting with artificial media.
Hazardous Supplies Value (HazMat) applies if shipped by Air.
PROCEDURE
Uncover: The following protocol has given good to nice outcomes with each sort of tissue sections. It
might should be optimized for a given tissue, half
thickness, and so forth.
Nationwide Diagnostics HISTO-CLEAR
1. Dewax and rehydrate slides
a) Immerse slides inside the next sequence of baths:
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
l 100% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 50% ethanol – 2 minutes
l Water – 2 minutes
2. Stain
a) Immerse slides in Hematoxylin reply for 10- 20 minutes.
b) Rinse 2 minutes in water or Scott’s Tapwater substitute.
c) Differentiate with 2-5 dips in 70% ethanol containing 0.1% HCl.
d) Immerse for two minutes in Scott’s Tapwater substitute.
e) Stain in 1% aqueous Eosin with 0.1 acetic acid for 10 minutes.
3. Dehydrate, clear and mount
a) Immerse slides inside the next sequence of
baths:
l 50% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 100% ethanol – 2 minutes
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
b) Mount coverslip with Histomount.
