The validation of a commercial enzyme-linked immunosorbent assay and the effect of freeze-thaw cycles of serum on the stability of cortisol and testosterone concentrations in Aceh cattle.

The validation of a commercial enzyme-linked immunosorbent assay and the effect of freeze-thaw cycles of serum on the stability of cortisol and testosterone concentrations in Aceh cattle.

Background: To get an accurate measurement of cortisol (C) and testosterone (T) in cattle Aceh, enzyme-linked immunosorbent assay (ELISA) commercial kits need to be carefully validated. Moreover, repeated freeze-thaw cycles during sample storage may affect the stability of hormones in serum. Here, we test the reliability of the measurement of the concentration of C and T in serum of cattle Aceh, were obtained using a commercial ELISA kit C and T C is designed to measure the concentration of human and T. Furthermore, we evaluated the effect of repeated freeze-thaw cycles on the stability of C and T concentrations in serum. Method: (. Cat no EIA-1887.) Commercial C and T (Cat no EIA-1559 ..) ELISA kit from DRG Instruments GmbH validated through the analysis of the validation test (ie, parallelism, accuracy, and precision) and the biology test validation (for C: effects of transport on the excretion of C, because T: T concentrations between bulls and cows).

To test the effect of freeze-thaw cycles, bovine serum was subjected to the following treatments: (i) remains frozen at -20 OC (control group); (Ii) is exposed to freeze-thaw cycles for two, four, six, and eight times (test group). Results: Parallelism, accuracy, and precision tests showed that both C and T ELISA kit adequately measured C and T in Aceh bovine serum. Post-transport C concentration was significantly higher than the pre-transport (p <0.05). The concentration of T in bulls was significantly higher than in cows (p <0.05). After four to eight cycles of freeze-thaw, their concentrations were significantly lower compared with the control group (all p <0.05).

In contrast, the concentration of T remains stable (all p> 0.05). Conclusion: Commercial C (EIA-1887) and T (EIA-1559) reliable ELISA test kit for measuring serum C and T, respectively, in cattle Aceh. Repeated freeze-thaw cycles significantly affect the stability of serum C, but not for T.

The validation of a commercial enzyme-linked immunosorbent assay and the effect of freeze-thaw cycles of serum on the stability of cortisol and testosterone concentrations in Aceh cattle.
The validation of a commercial enzyme-linked immunosorbent assay and the effect of freeze-thaw cycles of serum on the stability of cortisol and testosterone concentrations in Aceh cattle.

comparative assessment of commercial enzyme-linked immunosorbent assay and rapid diagnostic tests are used for diagnosis of dengue fever in India.

dengue fever diagnosis is routinely done by the detection of dengue virus (DENV) NS1 antigen and / or anti-DENV IgM antibody test using enzyme-linked immunosorbent assay (ELISA) and rapid diagnostic test (RDT). This study aimed to evaluate the quality of diagnostic tests being used in India for the identification of dengue DENV infection.During 2016 season (July to November) in Pune, India, comparative assessment of multiple immunoassay was performed using (i) WHO approved Panbio-Dengue-early – (NS1) -ELISA and Panbio-Dengue-IgM-capture-ELISA as a reference test, and (ii) Bayesian latent class analysis (BLCA) which assumes that no test is perfect.

Assays including J.Mitra-Dengue-NS1-Ag-MICROLISA (JME-NS1), J.Mitra-Dengue-IgM-MICROLISA (JME-IgM), and the two RDTs, ie, J.Mitra-Dengue-Day-1 -test (JM-RDT) and SD BIOLINE-Dengue-Duo (SDB-RDT). serum samples from patients with dengue diagnosis (n = 809) were tested using a diagnostic kit. The presence of NS1 and / or IgM taken as proof positive dengue diagnosis.Panbio-NS1 / IgM-ELISA identified 38.6 per patient per cent positive dengue fever.

ELISA kit for Rat Cystatin-C

EK2416 96 tests
EUR 469
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Cystatin-C in samples from serum, plasma, tissue homogenates and other biological fluids.

Cystatin C ELISA Kit (Rat) (OKIA00140)

OKIA00140 96 Wells
EUR 630
Description: Description of target: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L. ;Species reactivity: ;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.206 ng/ml

Cystatin C (CST3) (Rat) ELISA Kit

E4305-100
EUR 729

Rat Cystatin C(Cys-C)ELISA Kit

GA-E0153RT-48T 48T
EUR 354

Rat Cystatin C(Cys-C)ELISA Kit

GA-E0153RT-96T 96T
EUR 571

Rat Cys-C(Cystatin C) ELISA Kit

ER0891 96T
EUR 476.25
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 9.375pg/ml

Rat Cystatin C, Cys-C ELISA Kit

CSB-E08385r-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Rat Cystatin C, Cys-C in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Cystatin C, Cys-C ELISA Kit

1-CSB-E08385r
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Cystatin C, Cys-C in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Cystatin C(Cys-C)ELISA Kit

QY-E11499 96T
EUR 361

Rat Cystatin C,Cys-C ELISA Kit

CN-01845R1 96T
EUR 481

Rat Cystatin C,Cys-C ELISA Kit

CN-01845R2 48T
EUR 332

Cys-C ELISA Kit| Rat Cystatin C ELISA Kit

EF017675 96 Tests
EUR 689

ELISA kit for Rat Cys-C (Cystatin C)

E-EL-R0304 1 plate of 96 wells
EUR 377
Description: A sandwich ELISA kit for quantitative measurement of Rat Cys-C (Cystatin C) in samples from Serum, Plasma, Cell supernatant

Human Cystatin C ELISA Kit

DEIA2709 96T
EUR 876
Description: For the quantitative determination of human Cystatin C concentrations in cell culture supernates, serum, plasma, saliva, urine, and human milk.

Human Cystatin C ELISA kit

LF-EK50565 1×96T
EUR 648

Mouse Cystatin C ELISA kit

LF-EK50705 1×96T
EUR 648

Canine Cystatin C ELISA Kit

ELA-E0896d 96 Tests
EUR 928

Porcine Cystatin C ELISA Kit

ELA-E0896p 96 Tests
EUR 928

Rabbit Cystatin C ELISA Kit

ELA-E0896Rb 96 Tests
EUR 928

Rat Cystatin B ELISA Kit

ELA-E1853r 96 Tests
EUR 886

Cys-C ELISA Kit| Porcine Cystatin C ELISA Kit

EF016587 96 Tests
EUR 689

Cys-C ELISA Kit| Monkey Cystatin C ELISA Kit

EF012693 96 Tests
EUR 689

Cys-C ELISA Kit| Mouse Cystatin C ELISA Kit

EF012850 96 Tests
EUR 689

Mouse Cystatin C (CST3) ELISA Kit

abx570080-96tests 96 tests
EUR 668

Human Cystatin C (CST3) ELISA Kit

abx573769-96tests 96 tests
EUR 668

Cow Cystatin C (CST3) ELISA Kit

abx514333-96tests 96 tests
EUR 911

Human Cystatin C PicoKine ELISA Kit

EK0678 96 wells
EUR 456
Description: For quantitative detection of Human Cystatin C in cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine and human milk.

Mouse Cystatin C PicoKine ELISA Kit

EK0679 96 wells
EUR 456
Description: For quantitative detection of mouse Cystatin C in cell culture supernates, serum, plasma( heparin, EDTA) and urine.

Mouse Cst3/ Cystatin-C ELISA Kit

E0337Mo 1 Kit
EUR 546

Human CST3/ Cystatin-C ELISA Kit

E0582Hu 1 Kit
EUR 537

Human Cystatin C (CST3) ELISA Kit

abx050049-96tests 96 tests
EUR 770

Mouse Cystatin C (CST3) ELISA Kit

abx050050-96tests 96 tests
EUR 770

Pig Cystatin C (CST3) ELISA Kit

abx255460-96tests 96 tests
EUR 754

Cow Cystatin C (CST3) ELISA Kit

20-abx258834
  • EUR 7645.00
  • EUR 4074.00
  • EUR 942.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Cystatin C (CST3) ELISA Kit

abx254018-96tests 96 tests
EUR 668

Monkey Cystatin C (CST3) ELISA Kit

abx255351-96tests 96 tests
EUR 754

Rabbit Cystatin C (CST3) ELISA Kit

abx362196-96tests 96 tests
EUR 754

Sheep Cystatin C (CST3) ELISA Kit

abx364579-96tests 96 tests
EUR 926

Human Cystatin C (CST3) ELISA Kit

abx350046-96tests 96 tests
EUR 668

Dog Cystatin C (CST3) ELISA Kit

abx355449-96tests 96 tests
EUR 926

Chicken Cystatin C (CST3) ELISA Kit

abx356505-96tests 96 tests
EUR 754

Cystatin C ELISA Kit (Mouse) (OKBB00305)

OKBB00305 96 Tests
EUR 544
Description: Description of target: Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 10 pg/ml

ELISA kit for Human Cystatin-C

EK2417 96 tests
EUR 452
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Cystatin-C in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Mouse Cystatin-C

EK2418 96 tests
EUR 469
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Cystatin-C in samples from serum, plasma, tissue homogenates and other biological fluids.

Human CST3(Cystatin C) ELISA Kit

EH0110 96T
EUR 476.25
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Cystatin C ELISA Kit (Mouse) (OKIA00176)

OKIA00176 96 Wells
EUR 675
Description: Description of target: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.146 ng/ml

Cystatin C ELISA Kit (Monkey) (OKIA00184)

OKIA00184 96 Wells
EUR 622
Description: Description of target: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. ;Species reactivity: Monkey;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.330 ng/ml

Human Cystatin C ELISA kit (4X96T)

LF-EK50566 4×96T
EUR 2201

Mouse Cystatin C ELISA kit (4X96T)

LF-EK50706 4×96T
EUR 2201

Bovine Cystatin- C, CST3 ELISA KIT

ELI-03008b 96 Tests
EUR 928

Human Cystatin- C, CST3 ELISA KIT

ELI-03009h 96 Tests
EUR 824

Rabbit Cystatin- C, CST3 ELISA KIT

ELI-03010Ra 96 Tests
EUR 928

Mouse Cystatin- C, Cst3 ELISA KIT

ELI-03012m 96 Tests
EUR 865

Cystatin C (CST3) (Human) ELISA Kit

E4304-100
EUR 588

Cystatin C (CST3) (Porcine) ELISA Kit

E4306-100
EUR 729

Rabbit Cystatin C,Cys-C ELISA Kit

GA-E0065RB-48T 48T
EUR 326

Rabbit Cystatin C,Cys-C ELISA Kit

GA-E0065RB-96T 96T
EUR 524

Mouse Cystatin C(Cys-C)ELISA Kit

GA-E0355MS-48T 48T
EUR 336

Mouse Cystatin C(Cys-C)ELISA Kit

GA-E0355MS-96T 96T
EUR 534

Human Cystatin C(Cys-C)ELISA Kit

GA-E1121HM-48T 48T
EUR 289

Human Cystatin C(Cys-C)ELISA Kit

GA-E1121HM-96T 96T
EUR 466

Human Cystatin C,Cys-C ELISA Kit

201-12-1105 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Monkey Cys-C(Cystatin C) ELISA Kit

EMK0019 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Ape;Sensitivity: 0.094 ng/ml

Porcine Cys-C(Cystatin C) ELISA Kit

EP0046 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Pig;Sensitivity: 0.375 ng/ml

Mouse Cys-C(Cystatin C) ELISA Kit

EM0065 96T
EUR 476.25
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

Pig Cystatin C, Cys-C ELISA Kit

CSB-E13282p-24T 1 plate of 24 wells
EUR 165
Description: Quantitativecompetitive ELISA kit for measuring Pig Cystatin C, Cys-C in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Pig Cystatin C, Cys-C ELISA Kit

1-CSB-E13282p
  • EUR 946.00
  • EUR 5782.00
  • EUR 3060.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Pig Cystatin C, Cys-C in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Cystatin C, Cys-C ELISA Kit

CSB-E08384h-24T 1 plate of 24 wells
EUR 165
Description: Quantitative sandwich ELISA kit for measuring Human Cystatin C, Cys-C in samples from serum, plasma, urine, saliva, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Cystatin C, Cys-C ELISA Kit

1-CSB-E08384h
  • EUR 602.00
  • EUR 4238.00
  • EUR 2256.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Human Cystatin C, Cys-C in samples from serum, plasma, urine, saliva, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Cystatin C, Cys-C ELISA Kit

CSB-E08386m-24T 1 plate of 24 wells
EUR 165
Description: Quantitative sandwich ELISA kit for measuring Mouse Cystatin C, Cys-C in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Cystatin C, Cys-C ELISA Kit

1-CSB-E08386m
  • EUR 602.00
  • EUR 4238.00
  • EUR 2256.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Mouse Cystatin C, Cys-C in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Cystatin C(Cys-C)ELISA Kit

QY-E20429 96T
EUR 361

Human Cystatin C(Cys-C)ELISA Kit

QY-E04227 96T
EUR 361

Rabbit Cystatin C,Cys-C ELISA Kit

QY-E30235 96T
EUR 374

Canine Cystatin C(Cys-C) ELISA Kit

QY-E70167 96T
EUR 426

Rabbit Cystatin C,Cys-C ELISA Kit

CN-00652R1 96T
EUR 489

Rabbit Cystatin C,Cys-C ELISA Kit

CN-00652R2 48T
EUR 338

Mouse Cystatin- C, Cys-C ELISA Kit

ELA-E0896m 96 Tests
EUR 692

Mouse Cystatin C,Cys-C ELISA Kit

CN-02722M1 96T
EUR 489

Mouse Cystatin C,Cys-C ELISA Kit

CN-02722M2 48T
EUR 338

Human Cystatin C,Cys-C ELISA Kit

CN-03914H1 96T
EUR 492

Human Cystatin C,Cys-C ELISA Kit

CN-03914H2 48T
EUR 341

Rat Cystatin C (Cys-C) CLIA Kit

abx195493-96tests 96 tests
EUR 825

Rat Cystatin-S (CST4) ELISA Kit

abx556213-96tests 96 tests
EUR 668

Rat Cystatin A (CSTA) ELISA Kit

abx513060-96tests 96 tests
EUR 668

Rat Cystatin B (CSTB) ELISA Kit

abx518626-96tests 96 tests
EUR 668

Rat Cstb/ Cystatin-B ELISA Kit

E0245Ra 1 Kit
EUR 571

Rat Cystatin 8 (CST8) ELISA Kit

abx391169-96tests 96 tests
EUR 911

Rat Cystatin 3 (CST3) ELISA Kit

20-abx155418
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Cystatin A (CSTA) ELISA Kit

20-abx156928
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Cystatin 3 (CST3) ELISA Kit

DLR-CST3-Ra-48T 48T
EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cystatin 3 (CST3) in samples from serum, plasma, urine or other biological fluids.

Rat Cystatin 3 (CST3) ELISA Kit

DLR-CST3-Ra-96T 96T
EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Rat Cystatin 3 (CST3) in samples from serum, plasma, urine or other biological fluids.

Rat Cystatin 3 (CST3) ELISA Kit

RD-CST3-Ra-48Tests 48 Tests
EUR 511

Rat Cystatin 3 (CST3) ELISA Kit

RD-CST3-Ra-96Tests 96 Tests
EUR 709

Rat Cystatin 3 ELISA Kit (CST3)

RK03595 96 Tests
EUR 521

Rat Cystatin A ELISA Kit (CSTA)

RK04075 96 Tests
EUR 521

With Panbio-ELISA as a reference, all the less sensitive tests for the detection of IgM, whereas for NS1, JM-RDT less sensitive. For the combined diagnosis (second marker), all tests have low sensitivity (55.7 to 76.6%). According BLCA, Panbio-ELISA was 84 percent sensitive for NS1, 86 percent for IgM and 87 percent for the combined diagnosis. Thus, another test performance is substantially improved by BLCA; However, the sensitivity of both RDTs for detection of IgM remains unacceptable