The validation of a commercial enzyme-linked immunosorbent assay and the effect of freeze-thaw cycles of serum on the stability of cortisol and testosterone concentrations in Aceh cattle.
Background: To get an accurate measurement of cortisol (C) and testosterone (T) in cattle Aceh, enzyme-linked immunosorbent assay (ELISA) commercial kits need to be carefully validated. Moreover, repeated freeze-thaw cycles during sample storage may affect the stability of hormones in serum. Here, we test the reliability of the measurement of the concentration of C and T in serum of cattle Aceh, were obtained using a commercial ELISA kit C and T C is designed to measure the concentration of human and T. Furthermore, we evaluated the effect of repeated freeze-thaw cycles on the stability of C and T concentrations in serum. Method: (. Cat no EIA-1887.) Commercial C and T (Cat no EIA-1559 ..) ELISA kit from DRG Instruments GmbH validated through the analysis of the validation test (ie, parallelism, accuracy, and precision) and the biology test validation (for C: effects of transport on the excretion of C, because T: T concentrations between bulls and cows).
To test the effect of freeze-thaw cycles, bovine serum was subjected to the following treatments: (i) remains frozen at -20 OC (control group); (Ii) is exposed to freeze-thaw cycles for two, four, six, and eight times (test group). Results: Parallelism, accuracy, and precision tests showed that both C and T ELISA kit adequately measured C and T in Aceh bovine serum. Post-transport C concentration was significantly higher than the pre-transport (p <0.05). The concentration of T in bulls was significantly higher than in cows (p <0.05). After four to eight cycles of freeze-thaw, their concentrations were significantly lower compared with the control group (all p <0.05).
In contrast, the concentration of T remains stable (all p> 0.05). Conclusion: Commercial C (EIA-1887) and T (EIA-1559) reliable ELISA test kit for measuring serum C and T, respectively, in cattle Aceh. Repeated freeze-thaw cycles significantly affect the stability of serum C, but not for T.
The validation of a commercial enzyme-linked immunosorbent assay and the effect of freeze-thaw cycles of serum on the stability of cortisol and testosterone concentrations in Aceh cattle.
comparative assessment of commercial enzyme-linked immunosorbent assay and rapid diagnostic tests are used for diagnosis of dengue fever in India.
dengue fever diagnosis is routinely done by the detection of dengue virus (DENV) NS1 antigen and / or anti-DENV IgM antibody test using enzyme-linked immunosorbent assay (ELISA) and rapid diagnostic test (RDT). This study aimed to evaluate the quality of diagnostic tests being used in India for the identification of dengue DENV infection.During 2016 season (July to November) in Pune, India, comparative assessment of multiple immunoassay was performed using (i) WHO approved Panbio-Dengue-early – (NS1) -ELISA and Panbio-Dengue-IgM-capture-ELISA as a reference test, and (ii) Bayesian latent class analysis (BLCA) which assumes that no test is perfect.
Assays including J.Mitra-Dengue-NS1-Ag-MICROLISA (JME-NS1), J.Mitra-Dengue-IgM-MICROLISA (JME-IgM), and the two RDTs, ie, J.Mitra-Dengue-Day-1 -test (JM-RDT) and SD BIOLINE-Dengue-Duo (SDB-RDT). serum samples from patients with dengue diagnosis (n = 809) were tested using a diagnostic kit. The presence of NS1 and / or IgM taken as proof positive dengue diagnosis.Panbio-NS1 / IgM-ELISA identified 38.6 per patient per cent positive dengue fever.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: Description of target: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L. ;Species reactivity: ;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.206 ng/ml
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cystatin C,Cys-C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Rat Cystatin C, Cys-C in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat Cystatin C, Cys-C in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive ELISA for quantitative measurement of Rat Cystatin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cystatin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Cystatin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Cystatin C in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
With Panbio-ELISA as a reference, all the less sensitive tests for the detection of IgM, whereas for NS1, JM-RDT less sensitive. For the combined diagnosis (second marker), all tests have low sensitivity (55.7 to 76.6%). According BLCA, Panbio-ELISA was 84 percent sensitive for NS1, 86 percent for IgM and 87 percent for the combined diagnosis. Thus, another test performance is substantially improved by BLCA; However, the sensitivity of both RDTs for detection of IgM remains unacceptable
