ATpase assay

Key Decisions:

Non-radioactive, homogeneous assays
• Particular assays
• Helpful: minimal pattern preparation; quick protocols (1-2 hours )
• Price atmosphere pleasant: 100 assays;
• Excessive Throughput Screening relevant
• Validated: utilizing mammalian tissues, cells, pure fluids

BioVision affords full in vitro bioassays which might be utilized by a complete lot of worldwide scientists in pretty a few illness associated areas like most cancers, diabetes, weight points, coronary coronary heart points, liver illness, kidney illness, Alzheimer’s illness and infectious ailments, and so forth., together with completely totally different analysis areas equal to metabolism, neuroscience, epigenetics, oxidative stress, stem cells, protein extraction and pattern preparation, and so forth. Our high-quality bioassays present specificity, sensitivity, reproducibility and nonetheless pretty priced. Our mission is to ship revolutionary bioassays primarily based on forefront analysis to fulfill your scientific wants. BioVision’s bioassay kits could also be utilized with a few of the most well-liked and customary analysis instrumentation (i.e. microplate reader, movement cytometer, and microscopes).

Inorganic ions play pretty a few physiological roles in nature. Cations and anions take part in neurological transmission, muscle contraction, intra-cellular water transport, acid-base stability in blood, bone formation, vitality swap and storage. Moreover, they perform as cofactors or may be discovered as moieties inside
completely totally different necessary biomolecules. BioVision has a terrific portfolio of assays measuring inorganic ions together with assays for sodium, calcium, phosphate and a great deal of others.

 

BioVision’s intensive microplate-based assay portfolio affords pretty a few merchandise which may be utilized for the detection/quantification of enzymatic practice, quantification of express chemical analytes in pure samples and for
high-throughput inhibitor screening. Our merchandise revenue from mixtures of proprietary enzymes and chemical probes, fastidiously engineered and characterised for the detection of related targets. Our kits have been examined and validated utilizing exact pure samples (equal to blood, serum, plasma, urine, saliva, cell and tissue lysates and foodstuffs), guaranteeing that analytes are detectable in real-world pure matrices.

Pattern utility is presumably a vital problem when deciding on the correct product to your analysis wants. This may probably resolve the mandatory assay sensitivity and subsequently the favored detection methodology. Our hottest merchandise revenue from protected chromophores and fluorophores that present sturdy alerts over time. PicoProbe, our proprietary fluorophore, is little question a few of the delicate probes obtainable on the market. It’s going to in all probability detect as little as 20 pmoles of analyte per analyzed pattern accurately.

Metabolism performs a key function in numerous areas equal to physiology, cell biology, and medicines. Analysis in metabolism is dramatically elevated by the conclusion that many frequent human ailments equal to most cancers, diabetes, weight points, hypoglycemia, hypolipidemia, phenylketonuria, neurodegeneration, and so forth. are ensuing from perturbations in metabolic pathways. Understanding the metabolic variations and attainable outliers is thus a vital facet of disease-oriented analysis. Then as soon as extra, reactive oxygen species (ROS), produced endogenously on account of ordinary mobile metabolic capabilities or from exterior sources, poses a severe menace to cells since publicity to ROS may end up in severedamage to DNA, protein, and lipids. Figuring out express styles of oxidative damage is often the focus of curiosity  in numerous styles of cancers and totally different neurological ailments. Antioxidant defenses to chop again fluctuations in ROS are important to cell survival and dictate approaches to illness remedies to cut once more menace of most cancers or age-related purposeful deterioration.

 

Nonetheless, pure processes are bigger characterised by discovering out mobile interplay ensuing from exogenous components. Preclinical and Scientific Analysis depend on assays that take a look at utterly completely totally different features of mobile conduct (enchancment by way of the cell cycle, migration, invasion) and measure the content material materials supplies of key establishing blocks contained in the cell are often used to have a look at the correctly being of cells in customized and their response to totally different stimuli (migration inducers, cell cycle arrest medicine, ldl ldl ldl cholesterol transport inhibitors and so forth.).

 

Ca2+ / Mg2+ ATPase Assay Kit
abx097986-100Assays	Abbexa	100 Assays	EUR 510

ATPase/GTPase Assay Kit
Z5030043	Biochain	100 assays	EUR 757

Na+/K+ ATPase Microplate Assay Kit
DLSM0019	DL Develop	100 Assays	EUR 315
Description: Detection and Quantification of Na+/K+ ATPase Activity.

ATPase Activity Assay Kit (Colorimetric)
K417-100	Biovision	each	EUR 516

Ca2+/Mg2+ ATPase Microplate Assay Kit
DLSM0020	DL Develop	100 Assays	EUR 315
Description: Detection and Quantification of Ca2+/Mg2+ ATPase Activity.

QuantiChrom™ ATPase/GTPase Assay Kit
DATG-200	BioAssay Systems	200	EUR 389
Description: For quantitative determination of ATPase or GTPase activity and high-throughput screen for their inhibitors. Key Features: High sensitivity. Detection of 0.007 U/L ATPase or GTPase activity. Fast and convenient: single reagent, homogeneous "mix-and-measure" assay allows quantitation of enzyme activity within 30 minutes. Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: OD620nm. Samples: ATPase and GTPase. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.007 U/L.

E. coli DNA Helicase ATPase assay Kit Plus-100
DNAB100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x E. coli DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

E. coli DNA Helicase ATPase assay Kit Plus-500
DNAB500KE	ProFoldin	500 assays	EUR 1396.74
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x E. coli DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. aureus DNA Helicase ATPase assay Kit Plus-100
DNAB100KS	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x S. aureus DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

S. aureus DNA Helicase ATPase assay Kit Plus-500
DNAB500KS	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x S. aureus DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

E. coli gyrase ATPase assay Kit Plus (enzyme included)
T2A-100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes the reaction buffer, DNA, E. coli gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

H. influenzae DNA Helicase ATPase assay Kit Plus-100
DNAB100KH	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x H. influenzae DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay Kit Plus-100
DNAB100KN	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x S. pneumoniae DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

P. aeruginosa DNA Helicase ATPase assay Kit Plus-100
DNAB100KP	ProFoldin	100 assays	EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x P. aeruginosa DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

H. influenzae DNA Helicase ATPase assay Kit Plus-500
DNAB500KH	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x H. influenzae DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay Kit Plus-500
DNAB500KN	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x S. pneumoniae DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

P. aeruginosa DNA Helicase ATPase assay Kit Plus-500
DNAB500KP	ProFoldin	500 assays	EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x P. aeruginosa DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. aureus gyrase ATPase assay Kit Plus (enzyme included)
T2A-100KS	ProFoldin	100 assays	EUR 423.72
Description: This product includes the reaction buffer, DNA, S. aureus gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

E. coli Topo IV ATPase Assay Kit Plus (enzyme included)
T4A-100KE	ProFoldin	100 assays	EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x E. coli Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of E. coli Topo IV ATPase activity in a 384-well assay format.