Biovision DL-Serine Standard Curve

DL-Serine Assay Kit

DESCRIPTION

Serine is among the many many 20 naturally-occurring amino acids utilized by all organisms contained in the biosynthesis of proteins. Hsaving a single chiral middle, serine can exist as every of two stereoisomers (L-Serine and D-Serine). Along with its carry out as a protein organising block, L-Serine serves as a precursor to many important biomolecules, together with phosphatidylserine, sphingomyelin and the amino acids glycine and cysteine.

The non-proteogenic isomer D-Serine may even be synthesized from L-Serine contained in the mammalian ideas. Whereas most D-amino acids are solely current in hint parts in mammals, D-Serine is an important neurotransmitter that acts as a co-agonist of synaptic N-methyl-D-aspartate (NMDA) kind glutamate-gated ion channels. Impaired D-Serine metabolism might contribute to fairly just a few neuropsychiatric points, together with melancholy, schizophrenia, epilepsy and dementia. BioVision’s DL-Serine Assay Bundle permits for quantification of each L- and D-Serine in pure fluids and tissues.

Biovision DL-Serine Standard Curve
Biovision DL-Serine Normal Curve

The assay relies on the conversion of L-Serine to D-Serine, which is metabolized to an intermediate product that’s subsequently oxidized and reacts with a probe to kind a mild fluorophore (Ex/Em = 539/587 nm). Samples could also be divided and assayed concurrently for quantification of each D-Serine and full DL-Serine. The assay is solely not affected by physiological concentrations of assorted amino acids, is high-throughput adaptable and may detect lower than 1 μM Serine.

DL Serine Standard Curve
DL Serine Normal Curve
Cat # +Measurement Okay743-100
Measurement 100 assays
Bundle Abstract • Detection method- Fluorescence (Ex/Em 535/587 nm)
• Software program program- Measurement of Serine in pure samples paying homage to serum, plasma, CSF, and so forth.
Detection Methodology Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Features Estimation of Serine in assorted pure samples
Selections & Advantages • Easy, fast & helpful assay
• Restrict of detection: 1 µM
• Very particular, doesn’t work together with completely totally different common and non-standard amino acids
Bundle Components • Serine Assay Buffer
• Probe Reply
• Serine Racemase Enzyme Combine
• D-Serine Enzyme Combine
• Developer Enzyme Combine
• Pattern Cleanup Combine
• D-Serine Commonplace
Storage Situations -20°C
Provide Situations Gel Pack
USAGE For Analysis Use Solely! Not For Use in People.

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