HRP Conjugated Arachis

HRP Conjugated Arachis

Abstract

Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker  it strongly recognises essentially the most cancers specific T antigen (Galbeta1–>3GalNAc-), nonetheless not its sialylated mannequin. Nonetheless, an additional specificity within the route of Galbeta1–>4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA.

For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, fairly than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and completely different lectins of acknowledged specificity. Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccsharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA.

Arachis hypogaea (Peanut) Lectin (PNA) – HRP

Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is usually used for blotting, immunoassays and immunohistochemistry methods. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or launch of sunshine as a byproduct of the response. This product is out there in a stabilized liquid kind.For substrate use of HRP-labelled protein detection we offer our Intensive ChemiLuminescence (ICLTM) gear (SKU: 20810000). ICL is a two-component substrate that accommodates a gradual luminol reply with an enhancer and a gradual peroxide buffer reply. It has a robust light emission and a sensitivity at picogram diploma that is extraordinarily acceptable for the speedy detection of HRP-conjugated proteins. Advantages of chemiluminescent substrates over completely different detection methods comprises various exposures, blots is also reprobed, detects and quantitates quite a lot of protein concentrations, and yields largest sensitivity of any on the market method.We moreover provide 3, 3, 5, 5-Tetramethyl benzidine (TMB, SKU: 42000012) and TMB OneTM (TMB 1) HRP substrate for HRP labelled detection. TMB is an acceptable substrate for the immunoassay of horseradish peroxidase and is a noncarcinogenic by-product of benzidine with an absorbance at 450 nm. Oxidation of TMB by HRP yields a colored product. s TMB 1TM is a one component peroxidase substrate (SKU: 21530073).

arachis eylabs
arachis eylabs

Software program

Widespread Western Blot Protocol:

  • Glycoprotein sample dimension: 500ng
  • Lectin Focus: 0.1ug/ml
  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS internet web page gel
  3. Change gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Biochem/Physiol Actions

PNA would not agglutinate common human erythrocytes, nonetheless strongly agglutinates neuraminidase dealt with erythrocytes. PNA has potent anti-T train very like the anti-T antibody in human sera. The lectin might be utilized to inform aside between human lymphocyte subsets.

Unit Definition

One unit will kind 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.Zero at 20 °C.

Bodily kind

Accommodates sodium citrate

Preparation Discover

Prepared from peroxidase (P8375) using a modification of a broadcast method, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Arachis hypogaea lectin (PNA)

05-0116-10 10 mg
EUR 139.2

Arachis hypogaea lectin (PNA

05-0116-1000 1 g Ask for price

Arachis hypogaea lectin (PNA

05-0116-50 50 mg
EUR 376.8

Arachis hypogaea Arachin Ahy-3

1-CSB-EP737391ANE
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli

Arachis hypogaea Arachin Ahy-3

1-CSB-YP737391ANE
  • EUR 814.80
  • EUR 402.00
  • EUR 2606.40
  • EUR 1261.20
  • EUR 1730.40
  • EUR 522.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast

Arachis hypogaea Allergen Ara h 1, clone P17

1-CSB-EP331779ANE(A4)
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli

Arachis hypogaea Allergen Ara h 1, clone P17

1-CSB-EP331779ANE
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli

Arachis hypogaea Allergen Ara h 1, clone P17

1-CSB-YP331779ANE(A4)
  • EUR 703.20
  • EUR 358.80
  • EUR 2606.40
  • EUR 1080.00
  • EUR 1730.40
  • EUR 458.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast

Arachis hypogaea Allergen Ara h 1, clone P41B

1-CSB-CF337356ANE
  • EUR 1158.00
  • EUR 798.00
  • EUR 858.00
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system

CF568 Arachis hypogaes Lectin PNA (Peanut)

29061 1mg
EUR 234
Description: Minimum order quantity: 1 unit of 1mg

CF594 Arachis hypogaes Lectin PNA (Peanut)

29062 1mg
EUR 234
Description: Minimum order quantity: 1 unit of 1mg

CF488A Arachis hypogaes Lectin PNA (Peanut)

29060 1mg
EUR 234
Description: Minimum order quantity: 1 unit of 1mg

CF640R Arachis hypogaes Lectin PNA (Peanut)

29063 1mg
EUR 234
Description: Minimum order quantity: 1 unit of 1mg

Arachin Ahy-3 Antibody, HRP conjugated

1-CSB-PA737391LB01ANE
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Arachin Ahy-3. Recognizes Arachin Ahy-3 from Peanut. This antibody is HRP conjugated. Tested in the following application: ELISA

Arachin Ahy-3 Polyclonal Antibody, HRP Conjugated

A56271
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

QPCR Kit DNA Arachis hyp

MOL6482 EACH
EUR 1118.9

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