Direct PCR

Phire and Phusion Kits – Direct PCR

Advantages

  • Speedy amplification: The amplification payment of the polymerase is 6kb/min. 1kb fragment could be amplified inside 25min.
  • Prolonged fragment amplification: For plasmid, λ DNA and completely different easy templates, the polymerase can efficiently amplify > 20kb. For genome, the polymerase can efficiently amplify > 8kb. And for cDNA, the polymerase can efficiently amplify > 8kb.
  • Extreme specificity: With scorching start experience, the polymerase is 100% inactive underneath 50°C, and should solely be restored by heating at 95°C for 5min.
  • Extreme tolerance to impurities: Samples of whole blood, serum, cultured cells and urine could be instantly amplified with out prior DNA purification.
Direct PCR Reagent
Direct PCR Reagent

What’s Direct PCR?

Direct PCR (dPCR) is a method of DNA amplification instantly from an animal or plant tissue sample with out performing DNA isolation and purification steps. This technique considerably reduces experimental time, and worth in genotyping and high-volume duties. It moreover provides a larger selection when confronted with the challenges of amplifying a extremely small quantity of sample the place the purification step may doubtlessly lead to sample loss.

In some methods, dPCR works like typical PCR. Every methods depend on template DNA, primers and a grasp mix or PCR reagents, and every use a thermal cycler for amplification. The primary distinction between dPCR and customary PCR is the tailored buffers utilized in dPCR. The modified components of dPCR permit sturdy amplification whatever the presence of PCR inhibitors usually current in crude samples.

ALS SARS-CoV-2 RT-PCR 4G has been developed and produced utterly at ALS in Portugal and is designed primarily for export. This new gear is able to detect all acknowledged SARS-CoV-2 variants, along with British, Brazilian and South African variants, with out cross-reacting with completely different acknowledged related viruses or frequent respiratory pathogens. The collection of the genomic areas used as targets and the sequences of the primers and hydrolysis probes have been based totally on WHO, CDC and DGS solutions.

The ALS SARS-CoV-2 RT-PCR 4G gear is an in vitro diagnostic medical system (CE-IVD) that makes use of real-time PCR nucleic acid amplification experience for the detection of SARS-CoV-2 in medical samples from the upper respiratory tract of people that might or is not going to be suspected of getting COVID-19.

The gear makes use of the RT-PCR technique, combining amplification of certain areas of the viral genome with its detection by explicit hydrolysis probes. The SARS-CoV-2 genome aim areas are extraordinarily conserved and correspond to 2 explicit genes: ORF polyprotein (ORF1ab gene) and nucleocapsid phosphoprotein (N gene). The gear can also detect the gene that codifies the structural protein of the envelope (gene E) explicit to the Sarbecovirus subgenus.

Unit Definition

One unit is printed as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance inside 30 min at 74°C.

Description Code Specification Class Know-how
ALS SARS-CoV-2 RT-PCR Check out for surfaces ALS-B01-Adv RUO Meals /Water Virology Detection Precise-Time PCR
ALS SARS-CoV-2 RT-PCR Check out for Wastewater ALS-B02-Adv RUO Meals /Water Virology Detection Precise-Time PCR

 

Plant Direct PCR Kit

PD105-02 200 rxn (50 μl/rxn)
EUR 354

Direct PCR Complete Kit

S700 100 rcs
EUR 133

Direct PCR Complete Kit

S700-2 5x100 rcs
EUR 524

amfiSure Direct PCR Lysis Buffer

D0300-010 100ml
EUR 138

Blood Direct PCR Kit V2

PD103-01 50 rxn
EUR 169

Blood Direct PCR Kit V2

PD103-02 200 rxn
EUR 354

Mouse Direct PCR Kit (For Genotyping)

B40013 200 ractions
EUR 209
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B40015 500 ractions
EUR 409
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45012 1ml*5 (500 ractions)
EUR 280
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45013 1ml*20 (2000 ractions)
EUR 865
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

FastAmp Plant Direct PCR Kit - 250 Reactions

4612 1/EA
EUR 164

FastAmp Plant Direct PCR Kit - 1250 Reactions

4615 1/EA
EUR 518

Direct RT-PCR Kit wioRealStart DNA Polymerase

FYT507-100P 100 Preps Ask for price

Direct RT-PCR Kit wioRealStart DNA Polymerase

FYT507-50P 50 Preps Ask for price

Allele-In-One Human Blood Direct PCR Kit

ABP-PP-BD02100 100 RXNS Ask for price
    • Product line: One-step Genotyping
    • Brand:

Allele-In-One Human Blood Direct PCR Kit

ABP-PP-BD02500 500 RXNS Ask for price
    • Product line: One-step Genotyping
    • Brand:

Allele-In-One Mouse Tail Direct PCR Kit

ABP-PP-MT02100 100 RXNS Ask for price
    • Product line: One-step Genotyping
    • Brand:

Allele-In-One Mouse Tail Direct PCR Kit

ABP-PP-MT02500 500 RXNS Ask for price
    • Product line: One-step Genotyping
    • Brand:

Direct RT-PCR Kit wi RealStart DNA Polymerase

FYT506-100P 100 Preps Ask for price

Direct RT-PCR Kit wi RealStart DNA Polymerase

FYT506-50P 50 Preps; Ask for price

MULTIPLEX KIT PCR MASTITIS PCR kit

PCR-MPX218-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS

MULTIPLEX KIT PCR MASTITIS PCR kit

PCR-MPX218-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS

MULTIPLEX KIT PCR Babesia & Theileria PCR kit

PCR-MPX401-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria

MULTIPLEX KIT PCR Babesia & Theileria PCR kit

PCR-MPX401-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)

105-540 20 rcs x 50 µl
EUR 164

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)

105-542 100 rcs x 50 µl
EUR 549

 

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