The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom-made supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA gear depends on commonplace sandwich enzyme-linked immune-sorbent assay experience. A monoclonal antibody from mouse particular for BAFF-R has been pre-coated onto 96-well plates. Necessities (Expression system for frequent: NSO; Immunogen sequence: S7-A71) and attempt samples are added to the wells, a biotinylated detection polyclonal antibody from goat particular for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Delicate is added and unbound conjugates are washed away with PBS or TBS buffer.
Cell custom-made supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 10 pg/ml
Differ
78 pg/ml – 5000 pg/ml
Assay time
3h 30m
Assay measurement
A great deal of steps commonplace assay
Species reactivity
Reacts with: Human
bio human baff r
Notes
BAFF-R (furthermoregenerally called Tumor necrosis downside receptor superfamily member 13C or B-cell-activating downside receptor) is a protein that in folks is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating downside (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a kind III transmembrane protein containing a single extracellular phenylalanine-rich space. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Platform
Pre-coated microplate (12 x Eight appropriately strips)
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BAFF-R . This antibody is tested and proven to work in the following applications:
Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.
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