The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom-made supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA gear depends on commonplace sandwich enzyme-linked immune-sorbent assay experience. A monoclonal antibody from mouse particular for BAFF-R has been pre-coated onto 96-well plates. Necessities (Expression system for frequent: NSO; Immunogen sequence: S7-A71) and attempt samples are added to the wells, a biotinylated detection polyclonal antibody from goat particular for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Delicate is added and unbound conjugates are washed away with PBS or TBS buffer.
Cell custom-made supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 10 pg/ml
Differ
78 pg/ml – 5000 pg/ml
Assay time
3h 30m
Assay measurement
A great deal of steps commonplace assay
Species reactivity
Reacts with: Human
bio human baff r
Notes
BAFF-R (furthermoregenerally called Tumor necrosis downside receptor superfamily member 13C or B-cell-activating downside receptor) is a protein that in folks is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating downside (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a kind III transmembrane protein containing a single extracellular phenylalanine-rich space. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Platform
Pre-coated microplate (12 x Eight appropriately strips)
Description: Recombinant human BAFF-R (B cell-activating factor from the TNF family), also known as CD268, is a disulfide-linked monomeric protein consisting of 76 a.a. and migrates as an approximately 9 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human BAFF-R extracellular domain was expressed in E. coli.
Description: Recombinant human BAFF-R (B cell-activating factor from the TNF family), also known as CD268, is a disulfide-linked monomeric protein consisting of 76 a.a. and migrates as an approximately 9 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human BAFF-R extracellular domain was expressed in E. coli.
Description: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice.
Description: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice.
Description: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice.
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