Abm Qpcr Retrovirus

QPCR Retrovirus titration kit

One of the best ways it actually works

The qRT-PCR course of entails subjecting serial dilutions of your pattern and a administration RNA of acknowledged copy quantity (equipped) to one-step qRT-PCR. The retroviral copy quantity contained in your pattern can then be decided by evaluating its Ct worth to the identical previous curve.

RNA purification and DNase I treatment steps are important to the protocol so even viruses packaged utilizing transient transfections, which at all times embrace residual plasmid DNA, may be precisely quantified utilizing this gear.

Pay money for fastened retroviral transductions

A extreme benefit of retroviral gene-delivery methods is the ability to manage the extent of gene expression by adjusting the multiplicity of an an an infection (MOI) to dictate the variety of viral genomes that enter your objective cells. These titration kits permit you to make a fast correlation between the retroviral copy quantity and retroviral infectivity in your cell kind and in the long term permit you to administration the expression stage between experiments.

 

Description

abm’s qPCR Retrovirus Titer Gear is a one-step assay by qPCR which doesn’t require further lysis or reverse transcription (RT) steps, and may be accomplished beneath one hour. Designed to ship excessive sensitivity and specificity, the gear ensures minimal non-specific background and higher full effectivity as in contrast with comparable kits throughout the market. ROX reference dye is obtainable separate from the MasterMix, making this gear universally acceptable with most qPCR devices.

 

qPCR and One-Step RT MasterMixes
qPCR and One-Step RT MasterMixes

 

PRODUCT COMPONENT QUANTITY
BlasTaq 2X qPCR Titer MasterMix 1.25 ml
Primer Combine 100 rxn (200 µl)
Commonplace Administration DNA 50 µl
ROX Reference Dye 15 µl
Nuclease-Free H2O 2 x 1.zero ml

 

SKU G949
Unit amount 100 rxn
Storage State of affairs Retailer at -20°C

Virus Titration Kits

Our Titration Kits present a quick and easy method for titrating your virus. The kits make use of every a fast RNA or DNA extraction step, and resolve viral RNA/DNA titer utilizing qRT-PCR.

Benefits of abm’s titration kits:

  • No RNA purification — saves time and eliminates inaccuracy.
  • Prepared-to-use reagent combine — reduces variability.
  • qRT-PCR in a single step — further delicate and correct than fully completely different strategies.
  • No NTC amplification — Distinctive primer design completely eliminates non-specific amplification in NTCs.

We furthermore present On the spot Lentivirus Titration Participating in taking part in playing cards, which allow fast detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Using the lateral move into immunoassay method, viral titer yields are assessed immediately inside 10 minutes utilizing our three step protocol.

he Retro-X qRT-PCR Titration Gear supplies a quick and easy method for measuring retrovirus titer. The kits use a fast RNA purification step earlier than quantifying the variety of retroviral genome copies utilizing qRT-PCR and TB Inexperienced utilized sciences.

Fast willpower of retroviral titer

Whereas typical titration strategies require as loads as 10 days to finish, these titration kits require solely four hours and work with all MMLV-based retroviral vectors (not MSCV). Utilizing qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an an infection, permitting you to carry out each on the same day.

 

 

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

Retrovirus Packaging Kit

P905 NULL
EUR 0

Retrovirus Mini Purification Kit

K1307-10
EUR 544

Retrovirus Mini Purification Kit

K1307-20
EUR 914

Retrovirus Maxi Purification Kit

K1308-10
EUR 1295

Retrovirus Maxi Purification Kit

K1308-2
EUR 533

Retrovirus Maxi Purification Kit

K1308-4
EUR 860

QuickTiter Retrovirus Quantitation Kit

VPK-120 20 assays
EUR 711
Description: Our QuickTiter Retrovirus Quantitation Kit provides a quick method for determining retroviral titer. This assay measures the viral nucleic acid content of your retrovirus, and may be performed either before or after purification of your virus.

M13 Phage Titration ELISA Kit

55R-PRPHAGE 96 tests
EUR 693
Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles

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