RNA Purifications kit

Total RNA Purification Kits

Software program:

GenElute Full RNA Purification Tools is used for the extraction of entire RNA:

  • From full blood samples throughout the detection of Hepatitis C Virus using a reverse transcriptase-polymerase spiral response (RT-PSR) system.
  • From an in a single day custom of bacterial strains in TSB medium in determining the implications of curcumin and silymarin on virulence and carbapenemase genes amongst multidrug-resistant Escherichia coli scientific isolates.

Widespread Description:

Sigma′s GenElute Full RNA Purification Tools provides a speedy method for the isolation and purification of entire RNA from cultured animal cells, tissue samples, blood, plasma, serum, micro organism, yeast, fungi, vegetation and viruses. The package deal purifies all sizes of RNA, from large mRNA and ribosomal RNA all the best way right down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from totally different cell elements paying homage to proteins, with out utilizing phenol or chloroform. The purified RNA is of the perfect integrity, and will be utilized in fairly a couple of downstream functions along with precise time PCR, reverse transcription PCR, Northern blotting, RNase security and primer extension, and expression array assays.

  • Isolate true entire RNA along with siRNA and microRNA
  • Rapidly take away contaminating genomic DNA with out utilizing enzymes
  • No phenol or chloroform extractions
  • Purify high-quality RNA in 20 minutes
  • Extract RNA from as little as a single cell
  • Isolate from all types of specimens

The purification methods of our Full RNA Purification Kits range from pure extraction reagents all one of the best ways to spin columns and magnetic beads.

Starting with high-quality, pure, and intact entire RNA is necessary to many experiments (e.g. RT-PCR, qRT-PCR, array analysis, Northern blots, nuclease security assays, and RNA-sequencing). The desk below will present you how one can choose the exact Invitrogen product to purify entire RNA out of your specific sample form and sample measurement.

RNA purification kit
RNA purification package

Thermo Scientific GeneJET RNA Purification Tools is a straightforward and surroundings pleasant system for purification of entire RNA from mammalian cultured cells, tissue, human blood cells, micro organism, and yeast.

The package deal makes use of a silica-based membrane experience inside the kind of a useful spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides will likely be isolated with the GeneJET RNA Purification Tools in 15 minutes after the lysis step. The high-quality purified RNA will be utilized in quite a lot of downstream functions, along with RT-PCR, RT-qPCR, Northern blotting, and totally different RNA-based analyses.

RNA purification
RNA purification


• Widespread – will be utilized for every cell and tissue samples from quite a lot of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and microorganism

• Setting-friendly – extreme yields of the entire RNA.
• Fast – 15 minutes after lysis step
• Pure – an A260/280 ratio > 1.9
• Helpful – spin columns are capped and assembled with assortment tubes


• Fast extraction of extreme purity RNA applicable for all customary molecular biology procedures, along with:
• Northern blotting
• Nuclease security assay contains Proteinase Okay Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Assortment Tubes) Assortment Tubes (2 ml) Assortment Tubes (1.5 ml) Detailed Protocol

GenElute Full RNA Purification Tools has been used to:

  • Quantitative, real-time RT-PCR for every large mRNA and small RNA along with miRNA
  • RT-PCR for every large mRNA and small RNA along with miRNA
  • Expression profiling
  • Subsequent Expertise Sequencing for RNA and miRNA
  • miRNA from plasma for discovery
  • microRNA cloning and amplification
  • PCR-based virus detection
  • PCR-based viable micro organism detection
  • Northern blotting
  • RNase security
  • Primer extension
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR
  • Isolate entire RNA of rat kidney tissues

RNA Purification Kit

MB1006-50preps 50 preps
EUR 199.2

PureXtract RNA Purification Kit

R6111-050 50 prep
EUR 336

PureXtract RNA Purification Kit

R6111-200 200 prep
EUR 1048.8

RNA/DNA Purification Kit (Magnetic Bead)

DA0623 32 test/kit Ask for price

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 344

Ethanol, DEPC Treated 96% , For RNA Purification

E4500-050 500ml Ask for price

Ethanol, DEPC Treated 70% , For RNA Purification

E4600-050 500ml Ask for price

PCR Purification Kit

  • EUR 326.40
  • EUR 243.60
  • 200 rxns
  • 50 rxns

AfterTri-RNA Purification Mini Kit (200prep)

FAATR-001-1 200 preps
EUR 310.8

After Tri-RNA Purification Mini Kit (50prep)

FAATR-001 50 preps
EUR 175.2

Lysosome Purification Kit

K235-50 each
EUR 601.2

SeraMir Exosome RNA Purification Column kit (20 exoRNA columns)

RA808A-1 20 preps
EUR 344

HCV Mini Purification Kit

K1309-10 each
EUR 802.8

HCV Mini Purification Kit

K1309-20 each
EUR 1096.8

HCV Maxi Purification Kit

K1310-10 each
EUR 1683.6

HCV Maxi Purification Kit

K1310-2 each
EUR 639.6

HCV Maxi Purification Kit

K1310-4 each
EUR 1083.6

Adenovirus Purification Kit

K1459-100 100 Preps
EUR 1244.4

Adenovirus Purification Kit

K1459-20 20 Preps
EUR 727.2

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