A uniform quantitative enzyme-linked immunosorbent assay for Coxsackievirus A16 antigen in vaccine

A uniform quantitative enzyme-linked immunosorbent assay for Coxsackievirus A16 antigen in vaccine

Coxsackievirus A16 (CV-A16), one of the major etiological agent of hand, foot and mouth disease (HFMD), causing outbreaks of disease in young children throughout the world. In order to promote the prevention and control of HFMD, research and development of vaccines CV-A16 has been done in China. However, due to lack of CV-A16 antigen detection methods are recognized, evaluation and quality control (QC) the effectiveness of the vaccine are very limited.

In this study, we established enzyme-linked immunosorbent assay quantitatively (Q-ELISA) to determine the concentration of CV-A16 antigen in a vaccine that can be applied in the manufacture in China. A neutralizing antibody 16E1 is used as a capture antibody that can bind to a variety of CV-A16 antigen of subgenotypes different, and antiserum from rabbits immunized CV-A16-HRP conjugated by which to detect and measure the CV-A16 antigen.

Q-ELISA validated for specificity, linearity, accuracy, precision and durability by using CV-A16 antigen a national standard (NS). In addition, we utilize Q-ELISA to measure the content of bulks vaccine antigen from six manufacturers and products among others from the manufacturers. The results showed that the Q-ELISA can meet QC requirements for all manufacturers involved.

THMs, HAAs and NAs production from culturable microorganisms in pipeline network by ozonation, chlorination, chloramination and joint disinfection strategies

dog heartworm and heat treatment: Evaluation using enzyme-linked immunosorbent assay either by (ELISA) and sera dog with heartworm infection status confirmed

Heat treatment serum has shown an increase in heart worm antigen detection in dog serum protected without knowing the true infection status of the animal and the dog confirmed experimentally infected with heartworm. Utilizing sera archived status necropsy heartworm infection is confirmed (n = 665) and a micro-titer well by antigen ELISA assay, this study evaluated how the composition of heartworm infection affect the results of the test antigen pre- and post-treatment of heat, determined the change of sensitivity of the test antigen and specificity and the application of optical density values.

The composition of the present heartworm infection in dogs with sera initially testing negative antigen consists of dead infection by 1/34 (2.9%), adult 10/34 (29.4%), males only 7/34 (20.6% ), women only 5/34 (14.7%), and mixed sex infections 11/34 (32.4%) with 2-62 heartworms which 6 are microfilaremic.

The composition of the remaining antigen negative heartworm infection post-heat treatment consists of dead infection by 1/14 (7.1%), adults 9/14 (64.3%), males only 2/14 (14.3%), and genital infections mixture 2/14 (14.3%) with 6 and 62 heartworms which one is microfilaremic. Overall sensitivity for all infections, adult heartworms and adult females before heat treatment was 86.9%, 90.7%, and 93.3% and after heat treatment sensitivity increased to 94.6%, 98.4%, and 99.2% respectively.

Human Myoglobin ELISA kit

E01M0357-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Myoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

human Myoglobin ELISA Kit

E65E2007 96T
EUR 595

Human Myoglobin ELISA Kit

EK1830 96 tests
EUR 414

Human Myoglobin ELISA Kit

MBS006477-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human Myoglobin ELISA Kit

MBS006477-48StripWells 48-Strip-Wells
EUR 550

Human Myoglobin ELISA Kit

MBS006477-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human Myoglobin ELISA Kit

MBS006477-96StripWells 96-Strip-Wells
EUR 765

Human Myoglobin ELISA Kit

IHUMYOKT each
EUR 431
Description: Human Myoglobin ELISA Kit

Human Myoglobin ELISA Kit

IHUMBKT each
EUR 538
Description: Human Myoglobin ELISA Kit

Human Myoglobin ELISA Kit

GWB-AF81CB 96 well Ask for price

Human Myoglobin ELISA Kit

MBS9425687-5x96Tests 5x96Tests
EUR 2070

Human Myoglobin ELISA Kit

MBS9425687-96Tests 96Tests
EUR 455

Human Myoglobin ELISA Kit

MBS763854-10x96StripWells 10x96-Strip-Wells
EUR 3900

Human Myoglobin ELISA Kit

MBS763854-48StripWells 48-Strip-Wells
EUR 340

Human Myoglobin ELISA Kit

MBS763854-5x96StripWells 5x96-Strip-Wells
EUR 2045

Human Myoglobin ELISA Kit

MBS763854-96StripWells 96-Strip-Wells
EUR 455

Human Myoglobin ELISA Kit

MBS732828-48StripWellsCompetitive 48-Strip-Wells-(Competitive)
EUR 485

Human Myoglobin ELISA Kit

MBS732828-48StripWellsSandwich 48-Strip-Wells-(Sandwich)
EUR 485

Human Myoglobin ELISA Kit

MBS732828-5x96StripWellsCompetitive 5x96-Strip-Wells-(Competitive)
EUR 3020

Human Myoglobin ELISA Kit

MBS732828-96StripWellsCompetitive 96-Strip-Wells-(Competitive)
EUR 690

Human Myoglobin ELISA Kit

MBS732828-96StripWellsSandwich 96-Strip-Wells-(Sandwich)
EUR 690

Human Myoglobin ELISA Kit

MBS8579474-5x96Tests 5x96Tests
EUR 3190

Human Myoglobin ELISA Kit

MBS8579474-96Tests 96Tests
EUR 695

Human Myoglobin ELISA Kit

MBS8415018-1Kit 1Kit
EUR 760

Human Myoglobin ELISA Kit

MBS8415018-5x1Kit 5x1Kit
EUR 3480

Human Myoglobin ELISA Kit

MBS289896-10x96StripWells 10x96-Strip-Wells
EUR 3290

Human Myoglobin ELISA Kit

MBS289896-48StripWells 48-Strip-Wells
EUR 340

Human Myoglobin ELISA Kit

MBS289896-5x96StripWells 5x96-Strip-Wells
EUR 1800

Human Myoglobin ELISA Kit

MBS289896-96StripWells 96-Strip-Wells
EUR 435

Human Myoglobin (MB) ELISA Kit

20-abx152437
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Myoglobin (MB) ELISA Kit

abx252858-96tests 96 tests
EUR 764.4

Human Myoglobin (MB) ELISA Kit

abx573468-96tests 96 tests
EUR 764.4

Human MB/ Myoglobin ELISA Kit

E2800Hu 1 Kit
EUR 515

Human Myoglobin (MB) ELISA Kit

AE34161HU-48Tests 48 Tests
EUR 360
Description: Human (Homo sapiens)

Human Myoglobin (MB) ELISA Kit

AE34161HU-96Tests 96 Tests
EUR 680
Description: Human (Homo sapiens)

Human MB / Myoglobin ELISA Kit

E0480h 96T
EUR 536

Human Myoglobin,MB ELISA KIT

E1241Hu-1096T 10*96T
EUR 4122

Human Myoglobin,MB ELISA KIT

E1241Hu-48wells 48 wells
EUR 300

Human Myoglobin,MB ELISA KIT

E1241Hu-596T 5*96T
EUR 2061

Human Myoglobin,MB ELISA KIT

E1241Hu-96wells 96 wells
EUR 458

Human Myoglobin (MB) ELISA Kit

KTE61705-48T 48T
EUR 359
Description: This Human Myoglobin (MB) ELISA Kit employs a two-site sandwich ELISA to quantitate MB.

Human Myoglobin (MB) ELISA Kit

KTE61705-96T 96T
EUR 619
Description: This Human Myoglobin (MB) ELISA Kit employs a two-site sandwich ELISA to quantitate MB.

Human Myoglobin (MB) ELISA Kit

KTE61705-96T5 96 T×5
EUR 2809
Description: This Human Myoglobin (MB) ELISA Kit employs a two-site sandwich ELISA to quantitate MB.

Human Myoglobin (MB) ELISA Kit

KTE61705-96T50 96 T×50
EUR 26209
Description: This Human Myoglobin (MB) ELISA Kit employs a two-site sandwich ELISA to quantitate MB.

Human Myoglobin (MB) ELISA Kit

RK09255 96T
EUR 280

Human Myoglobin (MB) ELISA Kit

AE34161HU-48T 48T
EUR 360
Description: Human (Homo sapiens)

Human Myoglobin (MB) ELISA Kit

AE34161HU-96T 96T
EUR 680
Description: Human (Homo sapiens)

Human Myoglobin (MB) ELISA Kit

MBS9715369-48Tests 48Tests
EUR 350

Human Myoglobin (MB) ELISA Kit

MBS9715369-5x96Tests 5x96Tests
EUR 1940

Human Myoglobin (MB) ELISA Kit

MBS9715369-96Tests 96Tests
EUR 520

Human Myoglobin ELISA Kit (MYO)

MBS9137438-5x96Tests 5x96Tests
EUR 2615

Human Myoglobin ELISA Kit (MYO)

MBS9137438-96Tests 96Tests
EUR 570

Human Myo(Myoglobin) ELISA Kit

EH0347 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml

Human Myo(Myoglobin) ELISA Kit

EKF57132-48T 48T
EUR 396.9

Human Myo(Myoglobin) ELISA Kit

EKF57132-5x96T 5x96T
EUR 2693.25

Human Myo(Myoglobin) ELISA Kit

EKF57132-96T 96T
EUR 567

Human MYO (Myoglobin) ELISA Kit

EKE60124-5x96T 5x96T
EUR 2667.6

Human MYO (Myoglobin) ELISA Kit

EKE60124-96T 96T
EUR 561.6

Human Myo(Myoglobin) ELISA Kit

E39EH0347 96T
EUR 595

Human MYO(Myoglobin) ELISA Kit

ELK1211-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYO in the samples is then determined by comparing the OD of the samples to the standard curve.

Human MYO(Myoglobin) ELISA Kit

ELK1211-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYO in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Myo(Myoglobin) ELISA Kit

ELK1357-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Myo. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Myo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Myo, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Myo in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Myo(Myoglobin) ELISA Kit

ELK1357-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Myo. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Myo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Myo, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Myo in the samples is then determined by comparing the OD of the samples to the standard curve.

Human MYO(Myoglobin) ELISA Kit

RE2746H-48wells 48 wells
EUR 116.55

Human MYO(Myoglobin) ELISA Kit

RE2746H-96wells 96 wells
EUR 161.55

Human MYO (Myoglobin) ELISA Kit

MBS8800213-10x96StripWells 10x96-Strip-Wells
EUR 3130

Human MYO (Myoglobin) ELISA Kit

MBS8800213-48StripWells 48-Strip-Wells
EUR 315

Human MYO (Myoglobin) ELISA Kit

MBS8800213-5x96StripWells 5x96-Strip-Wells
EUR 1710

Human MYO (Myoglobin) ELISA Kit

MBS8800213-96StripWells 96-Strip-Wells
EUR 385

Human Myo (Myoglobin) ELISA Kit

MBS8800359-10x96StripWells 10x96-Strip-Wells
EUR 3130

Human Myo (Myoglobin) ELISA Kit

MBS8800359-48StripWells 48-Strip-Wells
EUR 315

Human Myo (Myoglobin) ELISA Kit

MBS8800359-5x96StripWells 5x96-Strip-Wells
EUR 1710

Human Myo (Myoglobin) ELISA Kit

MBS8800359-96StripWells 96-Strip-Wells
EUR 385

Human MYO (Myoglobin) ELISA Kit

MBS2567291-10x96Tests 10x96Tests
EUR 3265

Human MYO (Myoglobin) ELISA Kit

MBS2567291-24Tests 24Tests
EUR 225

Human MYO (Myoglobin) ELISA Kit

MBS2567291-48Test 48Test
EUR 350

Human MYO (Myoglobin) ELISA Kit

MBS2567291-5x96Test 5x96Test
EUR 1655

Human MYO (Myoglobin) ELISA Kit

MBS2567291-96Tests 96Tests
EUR 395

Human MYO (Myoglobin) ELISA Kit

MBS2515596-10x96Tests 10x96Tests
EUR 3265

Human MYO (Myoglobin) ELISA Kit

MBS2515596-24Tests 24Tests
EUR 225

Human MYO (Myoglobin) ELISA Kit

MBS2515596-48Test 48Test
EUR 350

Human MYO (Myoglobin) ELISA Kit

MBS2515596-5x96Test 5x96Test
EUR 1655

Human MYO (Myoglobin) ELISA Kit

MBS2515596-96Tests 96Tests
EUR 395

Human MYO(Myoglobin) ELISA Kit

RE2746H-48wellsplate 48 wells plate
EUR 126

Human MYO(Myoglobin) ELISA Kit

RE2746H-96wellsplate 96 wells plate
EUR 180

Myoglobin (Human) ELISA Kit

E4326-100 each
EUR 874.8

Nori® Human Myoglobin ELISA Kit

GR111252 96-well
EUR 461

Myoglobin ELISA Kit (Human) (OKIA00078)

OKIA00078 96 Wells
EUR 606
Description: Description of target: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.540 ng/ml

Human Myoglobin (MYO) ELISA Kit

DLR-MYO-Hu 96T
EUR 272
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Myoglobin (MYO) ELISA Kit

DLR-MYO-Hu-48T 48T
EUR 414
Description: A sandwich quantitative ELISA assay kit for detection of Human Myoglobin (MYO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Myoglobin (MYO) ELISA Kit

DLR-MYO-Hu-96T 96T
EUR 524.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Myoglobin (MYO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Myoglobin (MYO) ELISA Kit

DL-MYO-Hu 96T
EUR 259
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Myoglobin (MYO) ELISA Kit

EKL59275-5x96T 5x96T
EUR 2229.18

Human Myoglobin (MYO) ELISA Kit

EKL59275-96T 96T
EUR 469.3

Human Myoglobin(MYO) Elisa Kit

EK711221 96 Wells
EUR 0.15

Human Myoglobin (MYO) ELISA Kit

EKN47189-48T 48T
EUR 205.38

Human Myoglobin (MYO) ELISA Kit

EKN47189-5x96T 5x96T
EUR 1393.65

Human Myoglobin (MYO) ELISA Kit

EKN47189-96T 96T
EUR 293.4

Human Myoglobin,MYO ELISA Kit

YLA1754HU-48T 48T Ask for price

Human Myoglobin,MYO ELISA Kit

YLA1754HU-96T 96T Ask for price

Human Myoglobin (MYO) ELISA Kit

EKU06078-48T 48T
EUR 301.63

Human Myoglobin (MYO) ELISA Kit

EKU06078-5x96T 5x96T
EUR 2046.78

Human Myoglobin (MYO) ELISA Kit

EKU06078-96T 96T
EUR 430.9

Human Myoglobin (MYO) ELISA Kit  

NSL1812Hu 96 wells
EUR 468

Human Myoglobin (MYO) ELISA Kit

RK01912 96 Tests
EUR 280

Human Myoglobin (MYO) ELISA Kit

RDR-MYO-Hu-48T 48T
EUR 279.3
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Myoglobin (MYO) ELISA Kit

RDR-MYO-Hu-48Tests 48 Tests
EUR 408

Human Myoglobin (MYO) ELISA Kit

RDR-MYO-Hu-96T 96T
EUR 399
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Myoglobin (MYO) ELISA Kit

RDR-MYO-Hu-96Tests 96 Tests
EUR 558

Human Myoglobin (MYO) ELISA Kit

RD-MYO-Hu-48T 48T
EUR 266
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Myoglobin (MYO) ELISA Kit

RD-MYO-Hu-48Tests 48 Tests
EUR 392.4

Human Myoglobin (MYO) ELISA Kit

RD-MYO-Hu-96T 96T
EUR 380
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Myoglobin (MYO) ELISA Kit

RD-MYO-Hu-96Tests 96 Tests
EUR 534

Human Myoglobin (MYO) ELISA Kit

SEA480Hu-10x96wellstestplate 10x96-wells test plate
EUR 3164.52
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myoglobin (MYO) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myoglobin (MYO) ELISA Kit

SEA480Hu-1x48wellstestplate 1x48-wells test plate
EUR 365.78
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myoglobin (MYO) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myoglobin (MYO) ELISA Kit

SEA480Hu-1x96wellstestplate 1x96-wells test plate
EUR 471.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myoglobin (MYO) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myoglobin (MYO) ELISA Kit

SEA480Hu-5x96wellstestplate 5x96-wells test plate
EUR 1760.04
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myoglobin (MYO) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myoglobin (MYO) ELISA Kit

4-SEA480Hu
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  • Ask for price
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Myoglobin (MYO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Myoglobin (MYO) ELISA Kit

MBS2020488-10x96StripWells 10x96-Strip-Wells
EUR 3305

Human Myoglobin (MYO) ELISA Kit

MBS2020488-24StripWells 24-Strip-Wells
EUR 260

Human Myoglobin (MYO) ELISA Kit

MBS2020488-48StripWells 48-Strip-Wells
EUR 350

Human Myoglobin (MYO) ELISA Kit

MBS2020488-5x96StripWells 5x96-Strip-Wells
EUR 1780

Human Myoglobin (MYO) ELISA Kit

MBS2020488-96StripWells 96-Strip-Wells
EUR 445

Human Myoglobin (MYO) ELISA Kit

MBS450752-10x96StripWells 10x96-Strip-Wells
EUR 3330

Human Myoglobin (MYO) ELISA Kit

MBS450752-48StripWells 48-Strip-Wells
EUR 315

Human Myoglobin (MYO) ELISA Kit

MBS450752-5x96StripWells 5x96-Strip-Wells
EUR 1700

Human Myoglobin (MYO) ELISA Kit

MBS450752-96StripWells 96-Strip-Wells
EUR 385

Human Myoglobin (MYO) ELISA Kit

MBS4501268-10x96Tests 10x96Tests
EUR 3420

Human Myoglobin (MYO) ELISA Kit

MBS4501268-48Tests 48Tests
EUR 320

Human Myoglobin (MYO) ELISA Kit

MBS4501268-5x96Tests 5x96Tests
EUR 1745

Human Myoglobin (MYO) ELISA Kit

MBS4501268-96Test 96Test
EUR 395

Human Myoglobin (MYO) ELISA Kit

MBS2701857-10x96StripWells 10x96-Strip-Wells
EUR 1760

Human Myoglobin (MYO) ELISA Kit

MBS2701857-24StripWells 24-Strip-Wells
EUR 205

Human Myoglobin (MYO) ELISA Kit

MBS2701857-48StripWells 48-Strip-Wells
EUR 245

Human Myoglobin (MYO) ELISA Kit

MBS2701857-5x96StripWells 5x96-Strip-Wells
EUR 940

Human Myoglobin (MYO) ELISA Kit

MBS2701857-96StripWells 96-Strip-Wells
EUR 290

Human Myoglobin (MYO) ELISA Kit

MBS268195-10x96StripWells 10x96-Strip-Wells
EUR 3040

A decrease in specificity from 97.8% -96.1% were observed after the heat treatment of heartworm negative sera. optical density values ​​for various intensities of infection are present in this study clearly show that the intensity of the result does not reflect the number of liver flukes present. These studies provide additional context for interpreting the results of the post-antigen hot dog from the animal shelter, demonstrating the diagnostic utility of optical density, and highlights the need to improve the diagnosis of heartworm.

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