Coxsackievirus A16 (CV-A16), one of the major etiological agent of hand, foot and mouth disease (HFMD), causing outbreaks of disease in young children throughout the world. In order to promote the prevention and control of HFMD, research and development of vaccines CV-A16 has been done in China. However, due to lack of CV-A16 antigen detection methods are recognized, evaluation and quality control (QC) the effectiveness of the vaccine are very limited.
In this study, we established enzyme-linked immunosorbent assay quantitatively (Q-ELISA) to determine the concentration of CV-A16 antigen in a vaccine that can be applied in the manufacture in China. A neutralizing antibody 16E1 is used as a capture antibody that can bind to a variety of CV-A16 antigen of subgenotypes different, and antiserum from rabbits immunized CV-A16-HRP conjugated by which to detect and measure the CV-A16 antigen.
Q-ELISA validated for specificity, linearity, accuracy, precision and durability by using CV-A16 antigen a national standard (NS). In addition, we utilize Q-ELISA to measure the content of bulks vaccine antigen from six manufacturers and products among others from the manufacturers. The results showed that the Q-ELISA can meet QC requirements for all manufacturers involved.
THMs, HAAs and NAs production from culturable microorganisms in pipeline network by ozonation, chlorination, chloramination and joint disinfection strategies
dog heartworm and heat treatment: Evaluation using enzyme-linked immunosorbent assay either by (ELISA) and sera dog with heartworm infection status confirmed
Heat treatment serum has shown an increase in heart worm antigen detection in dog serum protected without knowing the true infection status of the animal and the dog confirmed experimentally infected with heartworm. Utilizing sera archived status necropsy heartworm infection is confirmed (n = 665) and a micro-titer well by antigen ELISA assay, this study evaluated how the composition of heartworm infection affect the results of the test antigen pre- and post-treatment of heat, determined the change of sensitivity of the test antigen and specificity and the application of optical density values.
The composition of the present heartworm infection in dogs with sera initially testing negative antigen consists of dead infection by 1/34 (2.9%), adult 10/34 (29.4%), males only 7/34 (20.6% ), women only 5/34 (14.7%), and mixed sex infections 11/34 (32.4%) with 2-62 heartworms which 6 are microfilaremic.
The composition of the remaining antigen negative heartworm infection post-heat treatment consists of dead infection by 1/14 (7.1%), adults 9/14 (64.3%), males only 2/14 (14.3%), and genital infections mixture 2/14 (14.3%) with 6 and 62 heartworms which one is microfilaremic. Overall sensitivity for all infections, adult heartworms and adult females before heat treatment was 86.9%, 90.7%, and 93.3% and after heat treatment sensitivity increased to 94.6%, 98.4%, and 99.2% respectively.
Description: A competitive ELISA for quantitative measurement of Human Myoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYO in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYO in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Myo. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Myo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Myo, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Myo in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Myo. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Myo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Myo, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Myo in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: Description of target: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.540 ng/ml
Description: A sandwich quantitative ELISA assay kit for detection of Human Myoglobin (MYO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Myoglobin (MYO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myoglobin (MYO) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myoglobin (MYO) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myoglobin (MYO) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myoglobin (MYO) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Myoglobin (MYO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
A decrease in specificity from 97.8% -96.1% were observed after the heat treatment of heartworm negative sera. optical density values for various intensities of infection are present in this study clearly show that the intensity of the result does not reflect the number of liver flukes present. These studies provide additional context for interpreting the results of the post-antigen hot dog from the animal shelter, demonstrating the diagnostic utility of optical density, and highlights the need to improve the diagnosis of heartworm.
