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Enzyme-linked immunosorbent assays using virus-like particles containing mutations of conserved residues on envelope protein can distinguish three flavivirus infections

The recent outbreak of Zika virus (ZIKV) in areas endemic flavivirus-highlight the need for sensitive and specific serological tests. Previously, we and others reported the fusion loop lock (FL) residue and / or the BC loop (BCL) residues on the envelope protein of dengue virus (DENV) is recognized by flavivirus cross-reactive human monoclonal antibodies and polyclonal sera.

To improve ZIKV serodiagnosis, we employ wild type (WT) and FL or FL / BCL mutant virus-like particle (VLP) from ZIKV, DENV1 and the West Nile virus (WNV) in enzyme linked immunosorbent assay (ELISA), and tested convalescent- phase serum or plasma samples from reverse-transcription PCR-confirmed cases with different ZIKV, DENV and WNV infection. For the IgG ELISA, ZIKV WT-VLP had a sensitivity of 100% and a specificity of 52.9%, which increased to 83.3% by FL / BCL mutant VLP and 92.2% with relative optical density ratio WT mutant VLP.

Similarly, DENV1 and WNV-VLP WT has a sensitivity / specificity of 100% / 70.0% and 100% / 56.3%, respectively; specificity increased to 93.3% and 83.0% by FL mutant VLP. For IgM ELISA, ZIKV, DENV1 and WNV-VLP WT has a specificity of 96.4%, 92.3% and 91.4%, respectively, for the primary infection; improved specificity of 93.7 to 99.3% by FL or FL / BCL mutant VLP. An algorithm based on a combination of mutant and WT-VLP IgG ELISA is proposed for the main ZIKV discrimination, DENV and WNV infection and secondary DENV infections and ZIKV with previous DENV infection; This can be a powerful tool to better understand the pathogenesis of seroprevalence and ZIKV in an area where several flaviviruses co-circulate.

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serotype-specific detection of dengue virus nonstructural protein 1-based enzyme-linked immunosorbent assay validated by multi-national cohort

Background: Dengue virus (DENV) infection poses one the biggest obstacle to global human health. Four serotypes (DENV 1-4) present different symptoms and influence the immune responds next DENV infection, rendering surveillance, risk assessment, and control of diseases especially challenging. Early diagnosis and clinical management is very important and can be achieved by detection of DENV nonstructural protein 1 (NS1) in serum during the acute phase. However, some of the NS1-based test has been developed which is able to distinguish serotype DENV and none are currently available commercially.


Methodology / findings principles: We develop enzyme-linked immunosorbent assay (ELISA) to distinguish DENV-1-4 NS1 using serotype-specific pair of monoclonal antibodies. A total of 1,046 antibodies harvested from DENV-immunized mice and screened for antigen binding affinity. Clinical performance was evaluated using the ELISA-reaction confirmed 408 dengue polymerase chain samples obtained from patients in Brazil, Honduras, and India. The overall sensitivity of the test for the pan-DENV was 79.66% (325/408), and sensitivity to serotype DENV-1-4 were 79.1% (38/48), 80.41% (78/97), 100% ( 45/45) and 79.6% (98/123), respectively. Specificity reaches 94.07 to 100%.


Significance: Our research shows that strong antibody screening strategy that enables the development of ELISA-based NS1 serotype with a maximized specific and sensitive antigen binding. sensitive and specific assay was also used cohort most expansive to date, and where about half of Latin America, the geographic area is very under-represented in previous similar studies. ELISA test offers the potential for enhanced diagnosis during the acute phase of infection to help guide patient care and disease control.

Porcine Diamine Oxidase (DAO) ELISA Kit

RD-DAO-p-48Tests 48 Tests
EUR 555

Porcine Diamine Oxidase (DAO) ELISA Kit

RD-DAO-p-96Tests 96 Tests
EUR 771

Rat Diamine Oxidase (DAO) ELISA Kit

RD-DAO-Ra-48Tests 48 Tests
EUR 511

Rat Diamine Oxidase (DAO) ELISA Kit

RD-DAO-Ra-96Tests 96 Tests
EUR 709

Human Diamine Oxidase (DAO) ELISA Kit

RDR-DAO-Hu-48Tests 48 Tests
EUR 500

Human Diamine Oxidase (DAO) ELISA Kit

RDR-DAO-Hu-96Tests 96 Tests
EUR 692

Porcine Diamine Oxidase (DAO) ELISA Kit

RDR-DAO-p-48Tests 48 Tests
EUR 580

Porcine Diamine Oxidase (DAO) ELISA Kit

RDR-DAO-p-96Tests 96 Tests
EUR 807

Rat Diamine Oxidase (DAO) ELISA Kit

RDR-DAO-Ra-48Tests 48 Tests
EUR 534

Rat Diamine Oxidase (DAO) ELISA Kit

RDR-DAO-Ra-96Tests 96 Tests
EUR 742

Dao/ Rat Dao ELISA Kit

ELI-46592r 96 Tests
EUR 886

Mouse DAO ELISA Kit

EMD0039 96Tests
EUR 521

DAO ELISA Kit (Mouse) (OKEH05527)

OKEH05527 96 Wells
EUR 779
Description: Description of target: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. ;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Competitive ELISA;Sensitivity: 39 pg/mL

Mouse diamine oxidase(DAO)ELISA Kit    

GA-E0588MS-48T 48T
EUR 336

Mouse diamine oxidase(DAO)ELISA Kit    

GA-E0588MS-96T 96T
EUR 534

Mouse Diamine Oxidase ELISA Kit (DAO)

RK02737 96 Tests
EUR 521

Mouse DAO(Diamine Oxidase) ELISA Kit

EM0980 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 2.344 ng/ml

Mouse diamine oxidase, DAO ELISA Kit

CSB-E10090m-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Mouse diamine oxidase, DAO in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse diamine oxidase, DAO ELISA Kit

1-CSB-E10090m
  • EUR 946.00
  • EUR 5782.00
  • EUR 3060.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse diamine oxidase, DAO in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Diamine Oxidase (DAO) ELISA Kit

SEA656Mu-10x96wellstestplate 10x96-wells test plate
EUR 4391.16
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Diamine Oxidase (DAO) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Diamine Oxidase (DAO) ELISA Kit

SEA656Mu-1x48wellstestplate 1x48-wells test plate
EUR 449.27
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Diamine Oxidase (DAO) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Diamine Oxidase (DAO) ELISA Kit

SEA656Mu-1x96wellstestplate 1x96-wells test plate
EUR 598.96
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Diamine Oxidase (DAO) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Diamine Oxidase (DAO) ELISA Kit

SEA656Mu-5x96wellstestplate 5x96-wells test plate
EUR 2395.32
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Diamine Oxidase (DAO) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Diamine Oxidase (DAO) ELISA Kit

4-SEA656Mu
  • EUR 4442.00
  • EUR 2346.00
  • EUR 599.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Diamine Oxidase (DAO) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse diamine oxidase(DAO)ELISA Kit

QY-E20470 96T
EUR 361

Mouse diamine oxidase,DAO ELISA Kit

CN-02511M1 96T
EUR 436

Mouse diamine oxidase,DAO ELISA Kit

CN-02511M2 48T
EUR 287

Sheep DAO ELISA Kit

ESD0039 96Tests
EUR 521

Monkey DAO ELISA Kit

EMKD0039 96Tests
EUR 521

Porcine DAO ELISA Kit

EPD0039 96Tests
EUR 521

Rat DAO ELISA Kit

ERD0039 96Tests
EUR 521

Rabbit DAO ELISA Kit

ERTD0039 96Tests
EUR 521

Anserini DAO ELISA Kit

EAD0039 96Tests
EUR 521

Goat DAO ELISA Kit

EGTD0039 96Tests
EUR 521

Canine DAO ELISA Kit

ECD0039 96Tests
EUR 521

Chicken DAO ELISA Kit

ECKD0039 96Tests
EUR 521

Human DAO ELISA Kit

EHD0039 96Tests
EUR 521

Bovine DAO ELISA Kit

EBD0039 96Tests
EUR 521

Human DAO ELISA Kit

ELA-E13922h 96 Tests
EUR 824

DAO ELISA KIT|Human

EF005669 96 Tests
EUR 689

DAO ELISA Kit| Mouse Diamine Oxidase ELISA Kit

EF013561 96 Tests
EUR 689

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 1
EUR 202

ELISA kit for Mouse Diamine oxidase (DAO)

KTE70533-48T 48T
EUR 354
Description: Quantitative sandwich ELISA for measuring Mouse Diamine oxidase (DAO) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Diamine oxidase (DAO)

KTE70533-5platesof96wells 5 plates of 96 wells
EUR 2252
Description: Quantitative sandwich ELISA for measuring Mouse Diamine oxidase (DAO) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Diamine oxidase (DAO)

KTE70533-96T 96T
EUR 572
Description: Quantitative sandwich ELISA for measuring Mouse Diamine oxidase (DAO) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse DAO (Diamine Oxidase)

ELK5316 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Diamine Oxidase from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Mouse DAO (Diamine Oxidase)

E-EL-M0412 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse DAO (Diamine Oxidase) in samples from Serum, Plasma, Cell supernatant

Guinea Pig DAO ELISA Kit

EGD0039 96Tests
EUR 521

DAO ELISA Kit (Rabbit) (OKCA00937)

OKCA00937 96 Wells
EUR 956
Description: Description of target: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.;Species reactivity: Rabbit;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.312 U/mL

DAO ELISA Kit (Rat) (OKCA00969)

OKCA00969 96 Wells
EUR 956
Description: Description of target: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.195 mIU/mL

DAO ELISA Kit (Human) (OKCD09181)

OKCD09181 96 Wells
EUR 975
Description: Description of target: Recombinant Human D-Amino Acid Oxidase ;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.56ng/mL

DAO ELISA Kit (Human) (OKAN05362)

OKAN05362 96 Wells
EUR 792
Description: Description of target: This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.56 ng/mL

DAO ELISA Kit (Rat) (OKAN05493)

OKAN05493 96 Wells
EUR 792
Description: Description of target: ;Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.236 ng/mL

DAO ELISA Kit (Pig) (OKEH07970)

OKEH07970 96 Wells
EUR 1092
Description: Description of target: ;Species reactivity: Pig;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity:

Mouse Dao/ D-amino-acid oxidase ELISA Kit

E0378Mo 1 Kit
EUR 632

Mouse D- amino- acid oxidase, Dao ELISA KIT

ELI-16169m 96 Tests
EUR 865

Mouse Diamine Oxidase (DAO) CLIA Kit

20-abx491836
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Diamine Oxidase (DAO) CLIA Kit

abx195143-96tests 96 tests
EUR 825

Mouse DAO shRNA Plasmid

20-abx969943
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

DAO Recombinant Protein (Mouse)

RP127823 100 ug Ask for price

Dao ELISA Kit| Mouse D-amino-acid oxidase ELISA Kit

EF014627 96 Tests
EUR 689

Rat diamine oxidase(DAO)ELISA Kit

GA-E0233RT-48T 48T
EUR 317

Rat diamine oxidase(DAO)ELISA Kit

GA-E0233RT-96T 96T
EUR 496

Human diamine oxidase(DAO)ELISA Kit

GA-E0793HM-48T 48T
EUR 289

Human diamine oxidase(DAO)ELISA Kit

GA-E0793HM-96T 96T
EUR 466

Human diamine oxidase,DAO ELISA Kit

201-12-0777 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Diamine Oxidase ELISA Kit (DAO)

RK01242 96 Tests
EUR 521

Porcine Diamine Oxidase ELISA Kit (DAO)

RK03313 96 Tests
EUR 521

Rat Diamine Oxidase ELISA Kit (DAO)

RK03613 96 Tests
EUR 521

Porcine DAO(Diamine Oxidase) ELISA Kit

EP0047 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Pig;Sensitivity: 0.938 ng/ml

Rat DAO(Diamine Oxidase) ELISA Kit

ER0895 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 1.875 ng/ml

Bovine DAO(Diamine Oxidase) ELISA Kit

EB0118 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Cattle;Sensitivity: 9.375 ng/ml

Rat diamine oxidase(DAO) ELISA Kit

CSB-E12634r-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Rat diamine oxidase (DAO) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat diamine oxidase(DAO) ELISA Kit

1-CSB-E12634r
  • EUR 946.00
  • EUR 5782.00
  • EUR 3060.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat diamine oxidase(DAO) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human diamine oxidase, DAO ELISA Kit

CSB-E10137h-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human diamine oxidase, DAO in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human diamine oxidase, DAO ELISA Kit

1-CSB-E10137h
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human diamine oxidase, DAO in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Diamine Oxidase (DAO) ELISA Kit

SEA656Hu-10x96wellstestplate 10x96-wells test plate
EUR 4273.35
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Diamine Oxidase (DAO) in serum, plasma, tissue homogenates and other biological fluids.

Human Diamine Oxidase (DAO) ELISA Kit

SEA656Hu-1x48wellstestplate 1x48-wells test plate
EUR 439.57
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Diamine Oxidase (DAO) in serum, plasma, tissue homogenates and other biological fluids.

Human Diamine Oxidase (DAO) ELISA Kit

SEA656Hu-1x96wellstestplate 1x96-wells test plate
EUR 585.1
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Diamine Oxidase (DAO) in serum, plasma, tissue homogenates and other biological fluids.

Human Diamine Oxidase (DAO) ELISA Kit

SEA656Hu-5x96wellstestplate 5x96-wells test plate
EUR 2332.95
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Diamine Oxidase (DAO) in serum, plasma, tissue homogenates and other biological fluids.

Human Diamine Oxidase (DAO) ELISA Kit

4-SEA656Hu
  • EUR 4324.00
  • EUR 2283.00
  • EUR 586.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Diamine Oxidase (DAO) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Pig Diamine Oxidase (DAO) ELISA Kit

SEA656Po-10x96wellstestplate 10x96-wells test plate
EUR 5098.02
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Diamine Oxidase (DAO) in serum, plasma, tissue homogenates and other biological fluids.

Pig Diamine Oxidase (DAO) ELISA Kit

SEA656Po-1x48wellstestplate 1x48-wells test plate
EUR 507.48
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Diamine Oxidase (DAO) in serum, plasma, tissue homogenates and other biological fluids.

Pig Diamine Oxidase (DAO) ELISA Kit

SEA656Po-1x96wellstestplate 1x96-wells test plate
EUR 682.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Diamine Oxidase (DAO) in serum, plasma, tissue homogenates and other biological fluids.

Pig Diamine Oxidase (DAO) ELISA Kit

SEA656Po-5x96wellstestplate 5x96-wells test plate
EUR 2769.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Diamine Oxidase (DAO) in serum, plasma, tissue homogenates and other biological fluids.

Pig Diamine Oxidase (DAO) ELISA Kit

4-SEA656Po
  • EUR 5149.00
  • EUR 2720.00
  • EUR 683.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Diamine Oxidase (DAO) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat Diamine Oxidase (DAO) ELISA Kit

SEA656Ra-10x96wellstestplate 10x96-wells test plate
EUR 4626.78
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Diamine Oxidase (DAO) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Rat Diamine Oxidase (DAO) ELISA Kit

SEA656Ra-1x48wellstestplate 1x48-wells test plate
EUR 468.68
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Diamine Oxidase (DAO) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Rat Diamine Oxidase (DAO) ELISA Kit

SEA656Ra-1x96wellstestplate 1x96-wells test plate
EUR 626.68
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Diamine Oxidase (DAO) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Rat Diamine Oxidase (DAO) ELISA Kit

SEA656Ra-5x96wellstestplate 5x96-wells test plate
EUR 2520.06
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Diamine Oxidase (DAO) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Rat Diamine Oxidase (DAO) ELISA Kit

4-SEA656Ra
  • EUR 4677.00
  • EUR 2471.00
  • EUR 627.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Diamine Oxidase (DAO) in samples from serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat diamine oxidase(DAO)ELISA Kit

QY-E11419 96T
EUR 361

These results indicate that this ELISA is a promising aid in the early diagnosis of DENV-1-4 and supervision in areas of endemicity in addition to offering a comfortable monitoring for future vaccine interventions.

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