The relationship between intestinal permeability (IP) and body composition remains unclear. The gold standard test differential-absorption sugars are difficult to resolve, with the zonulin is being increasingly used as an independent biomarker IP. This pilot study aims to explore the relationship between small IP, zonulin concentration, and body composition in healthy adults. The ratio of urinary lactulose-rhamnose used to measure small IP.
Serum zonulin, lipopolysaccharide (LPS) and high sensitivity C-reactive protein (hs-CRP) were analyzed in serum. Body composition was measured using dual-energy X-ray absorptiometry and anthropometric measurements were collected. In total, 34 participants included (12 men, average age 28 years, body mass index 24 kg / m2, waist circumference 77cm). No correlation was observed between the ratio of lactulose-rhamnose and zonulin (r = -.016, P = 0.929).
Lactulose-rhamnose ratio shown a strong positive correlation with LPS (n 20, r = 0.536, P = 0.018) but did not correlate with measures of body composition. Instead, zonulin displayed a moderate positive correlation with waist circumference (r = 0.437, P = 0.042) in the female participants and hs-CRP (r = 0.485, P = 0.004) in all participants. These findings raise important considerations for small IP measurement, the guarantor of exploration in the study of more powerful that overcome the limitations of this study.
Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults
The performance of commercially available anti-HDV enzyme-linked immunosorbent assay in Taiwan
Background: Hepatitis D virus (HDV) infection is a major global health problem worldwide. There are approximately 15-20 million people worldwide infected with HDV. HDV infection usually causes increased mortality compared with infection with hepatitis B virus (HBV) only. However, testing for the detection of HDV is not widely available in Taiwan. Therefore, General Biologicals Corporation (GB) HDV Ab kit is developed to detect anti-HDV antibodies.
Methods: A total of 913 462 serum and EDTA-treated plasma samples obtained from HBsAg-positive individuals at three hospitals in Taiwan from June 2014 to November 2017. We are using three commercially available ELISA kit, DiaPro HDV Ab, DIASORIN ETI-AB-DELTAK GB -2 and HDV Ab, which is used in strict accordance with the manufacturer’s instructions.
Results: A comparative study of the results of HDV Ab GB kit and other commercial ELISA kit (DiaPro and DIASORIN) was conducted to determine their efficacy for the detection of anti-HDV. The results showed that the sensitivity of HDV Ab GB kit for serum and EDTA samples was 100% compared with that of DiaPro and DIASORIN kit, while specificity for serum and EDTA samples was 99.3 and 98.1%, respectively.
In addition, the overall results of the agreement HDV Ab GB kit for serum and EDTA samples was 99.3 and 98.3%, respectively. It should be noted that the performance of the kit GB HDV Ab is not affected by interference from triglycerides, bilirubin, hemoglobin, or mouse anti-human antibody. The detection limit of the kit GB HDV Ab is approximately 100-fold lower than the other two commercial kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Adropin (AD) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AD in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AD in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: A competitive ELISA for quantitative measurement of Human adropin(AD) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human adropin(AD) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human adropin(AD) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adropin (AD) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adropin (AD) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adropin (AD) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adropin (AD) in serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Adropin (AD) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A sandwich ELISA kit for detection of Adropin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Human Adropin (AD) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adropin (AD) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adropin (AD) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adropin (AD) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Adropin (AD) in serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of High Sensitive Human Adropin (AD) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A competitive ELISA for quantitative measurement of Rat adropin(AD) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat adropin(AD) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat adropin(AD) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine adropin(AD) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine adropin(AD) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine adropin(AD) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine adropin(AD) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine adropin(AD) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine adropin(AD) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AD in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AD in the samples is then determined by comparing the OD of the samples to the standard curve.
Conclusion: The GB HDV Ab kit, which presented similar sensitivity and specificity compared with both a certified anti-HDV kit, the kit will be suitable for the diagnosis of HDV in Taiwan.
