Many outbreaks of Burkholderia Cepacia Complex (BCC) infection are associated with contamination in personal care products (PCPS). This study aims to analyze the collection of BCC isolates in PCPs and assess preservative vulnerabilities, including Dimethoxy Dimethyl Hydantoin (DMDMH), Methylisothyzolinone-Chloromethylisothiazolinone (MIT / CMIT), and Methyl 4-hydroxybenzoate (MH). BCC isolates collected during the 3-year study period (2015-2017) was further examined by biochemical identification systems, phylogenetic analysis based on the sequence of nucleotide reca, and analysis of typing multilocus sequences.
Testing of the vulnerability of BCC bacterial bacteria is evaluated by the minimum inhibition concentration and minimum bactericidal concentration. A total of seven types of different sequences (STS) were identified, which originated from four different BCC species: Burkholderia Cenocepacia (ST621, ST258, and St. Novel), Burkholderia Lata (ST339 and ST336), Burkholderia Contaminans (ST482), Burkholderia Cepacia (ST922 ).
For DMDMH and MH, the maximum concentration permitted in accordance with cosmetic safety specifications (0.6% and 0.4%) is able to inhibit or kill all BCC strains, but 40% of BCC isolates can last higher than the concentration of MIT / maximum permitted / CMIT (from the mixture in the ratio of 3: 1 of the 5-chloro-2-methyl-4-isothiazoline-3-one and 2-methyl-4-isothiazolin-3-one). PCP contamination of BCC strains must be more attention to producers because of its diversity in molecular epidemiology and low vulnerability to preservatives such as MIT / CMIT.
Find new molecular targets from natural products known in the Silico target prediction
Natural products consist of rich reservoirs for innovative drug steering and is a constant source of bioactive compounds. To find the pharmacological target for new natural products or known to use a modern computer-assisted method is the current effort in drug discovery. However, natural treasures can be used more effectively. However, a reliable pipe for predictions of large-scale targets natural products is still rare. We developed a workflow in Silico consisting of four independent target and stand-alone prediction tools and evaluated its performance on Dihydrochalcones (DHCS) -The well-known natural product. Thus, we revealed four protein targets that were previously not reported for DHC, namely 5-lipoxygenase, cyclooloxygenase-1, 17β-hydroxysteroid dehydrogenase 3, and Aldo-Keto Reductase 1C3. In addition, we provide a comprehensive strategy on how to predict computing targets and guides using each tool.
Farmacophore-based virtual playback, molecular docking, molecular dynamics and MM-GBSA approach to identification of candidates for SARS-COV-2 inhibitors from natural product databases
Covid-19 caused by severe acute respiratory syndrome Coronavirus 2 (SARS-COV-2) mainly appeared in Wuhan, China, in December 2019. At present, there is no precise therapy and vaccination available for this disease, and increasing from day to day with a high mortality rate. Farmacophore-based virtual playback of the natural product database chosen followed by Glide Molecular Docking and the dynamics of the study of the main Protease SARS-COV-2 is investigated to identify potential ligands that can act as inhibitors. The molecule <b> SN00293542 </ b> and <b> SN00382835 </ b> reveals the highest docking score <b> -14.57 </ b> and <b> -12.42 kcal / mol, </ b> if Compared to Ligand Co-Crystal PDB-6Y2F (O6K) and 6W63 (X77) from SARS-COV-2 M <Soup> Pro </ soup>.
To further validate the Top score molecular interaction <b> SN00293542 </ b> and <b> SN00382835, </ b> Molecular Dynamics Study 100 NS is done. It shows that the complex protein-ligands are stable throughout the simulation period, and minimal backbone fluctuations have occurred in the system. Post-mm-GBSA analysis of molecular dynamics data shows free fastener energy – <b> 71.7004 +/- 7.98, -56.81 +/- 7.54 kcal / mol, </ b>. Computing studies identify several ligands that can act as potential inhibitors of SARS-COV-2 M <Soup> Pro </ soup>. Top ranking molecules <b> SN00293542, </ b> and <b> SN00382835 </ b> occupying the active target site, such main proteases from co-crystal ligands. These molecules may appear as ligands that promise SARS-COV-2 and thus need further detailed investigations. Communicated by Ramaswamy H. Sarma.
Immunological effects of chondrusarmatus Carragentens and low molecular degradation products are low
High molecular weight capability (HMW) κ- and λ-carrageenans from chondrusarmatus red marine algae and low molecular weight degradation products (LMWDPS) (0.7-20 and 10-170 kDa- respectively) to influence functional properties (motility and phagocytosis ) Murine Peritoneal Macrophag WeakSessesSedin This research as anin vitro and a week’s meal experiment.
We show that, with anexception of one, all barrage samples at 100 μg / ml increase cellular motility and dosage-dependently decrease in fagocytic activity; LMWDPS from λ-Carrageenan suppresses motility and has no effect on phagocytosis. Oral administration of all carpararacten samples with 100 μg / kg / day for 7 days for MiChad has no effect on their clinical appearance, weight, their weight, spleen or spleen department of the changes seen in their inner organs. All samples from the composition of the peritoneum cavity to macrophages. The consumption of LMWDPS from κ-Carrageenan results in the construction of leukopenia, but there are no changes to the relative WBC count introduced by one of the samples.
 Gel Tray |
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2398194 |
Atto |
2unit |
EUR 424.8 |
 TC Gel |
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CP048-005 |
GenDepot |
500 g |
EUR 278.4 |
TC Gel |
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CP048-010 |
GenDepot |
1 Kg |
EUR 416.4 |
 Gel Cutter |
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KS071012-11 |
Biochain |
12 |
EUR 106.8 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 Gel tray L |
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2322112 |
Atto |
5unit |
EUR 308.4 |
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Description: 2pcs/1set |
 Gel tray S |
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2322117 |
Atto |
5unit |
EUR 308.4 |
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Description: 2pcs/1set |
) Ketanserin (Vulketan Gel) |
|
E1KS2232 |
EnoGene |
50mg |
EUR 520.8 |
 Gel Breaker Tubes |
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KS071012-12 |
Biochain |
12 |
EUR 106.8 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 ViSafe RED Gel |
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S430 |
GeneOn |
500 µl |
EUR 175.2 |
ViSafe RED Gel |
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S430L |
GeneOn |
5x500 µl |
EUR 668.4 |
 ViSafe Green Gel |
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S435 |
GeneOn |
500 µl |
EUR 175.2 |
ViSafe Green Gel |
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S435L |
GeneOn |
5x500 µl |
EUR 668.4 |
 Aluminium Phosphate Gel |
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VAdv-Ly0001 |
Creative Biolabs |
250 mL |
EUR 1663.2 |
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Description: Aluminium Phosphate Gel 2%, Aluminum salt Vaccine adjuvant. |
 Aluminium Hydroxide Gel |
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VAdv-Ly0003 |
Creative Biolabs |
250 mL |
EUR 1663.2 |
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Description: Aluminium Hydroxide Gel 2%, Aluminum salt Vaccine adjuvant. |
) Short wave pass filter (SWP filter) |
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2130430 |
Atto |
1unit |
EUR 777.6 |
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Description: Shor t wave pass fi l t er 650nm |
) 96 well filter plate (no filter) |
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SD5006-EMPTY |
Bio Basic |
12UNIT |
EUR 104.89 |
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 Gel casting stand L |
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2322111 |
Atto |
5unit |
EUR 308.4 |
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Description: 1pc |
 Gel casting stand S |
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2322116 |
Atto |
5unit |
EUR 308.4 |
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Description: 1pc |
 Quick Gel Extraction Kit |
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20-abx098085 |
Abbexa |
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 Agarose, Low melting gel |
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AB0015 |
Bio Basic |
10g |
EUR 96.54 |
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 Anti-Flag Affinity Gel |
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B23101 |
Bimake |
1 ml Kit |
EUR 579.6 |
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Description: Anti-Flag Affinity Gel is a Protein A purIFied murine IgG2b Monoclonal antibody covalently attached to sepharose 4B wHICh could used on Flag fusion protein purIFication, IP and CO-IP. |
Anti-Flag Affinity Gel |
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B23102 |
Bimake |
5 ml Kit |
EUR 1742.4 |
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Description: Anti-Flag Affinity Gel is a Protein A purIFied murine IgG2b Monoclonal antibody covalently attached to sepharose 4B wHICh could used on Flag fusion protein purIFication, IP and CO-IP. |
 Drying Reagent, Silica Gel |
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DSG110 |
Bio Basic |
1Kg |
EUR 91.32 |
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 dam for 15cm gel |
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EHS3400-DAM |
Consort |
ea |
EUR 189.6 |
 dam for 20cm gel |
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EHS3500-DAM |
Consort |
ea |
EUR 199.2 |
 dam for 23.5cm gel |
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EHS3600-DAM |
Consort |
ea |
EUR 199.2 |
 Gel DNA Recovery Kit |
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GP05 |
GeneOn |
5 preps |
Ask for price |
Gel DNA Recovery Kit |
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GP100 |
GeneOn |
100 preps |
EUR 156 |
Gel DNA Recovery Kit |
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GP200 |
GeneOn |
200 preps |
EUR 226.8 |
Gel DNA Recovery Kit |
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GP50 |
GeneOn |
50 preps |
EUR 112.8 |
 mini tube gel insert |
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EVS1100-TUBEINSERT |
Consort |
ea |
EUR 493.2 |
 maxi tube gel insert |
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EVS1300-TUBEINSERT |
Consort |
ea |
EUR 604.8 |
) AmbiClean Kit (PCR & Gel) |
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GV05 |
GeneOn |
5 preps |
Ask for price |
AmbiClean Kit (PCR & Gel) |
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GV100 |
GeneOn |
100 preps |
EUR 153.6 |
AmbiClean Kit (PCR & Gel) |
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GV200 |
GeneOn |
200 preps |
EUR 226.8 |
AmbiClean Kit (PCR & Gel) |
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GV50 |
GeneOn |
50 preps |
EUR 109.2 |
 ProsiBlue Gel Staining Solution |
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P7300-050 |
GenDepot |
500ml |
EUR 208.8 |
 10x Gel Loading Buffer |
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TG4038 |
TopoGen |
1ml |
EUR 187.2 |
 Filter Holder |
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2006121 |
Atto |
2unit |
EUR 448.8 |
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Description: Opt i on |
 Vacuum Filter |
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Clamp |
FilterBio |
1set |
EUR 195.66 |
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Description: Anodized Aluminum Clamp |
Vacuum Filter |
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G-50 |
FilterBio |
1set |
EUR 237.42 |
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Description: Glass Funnel, 500ml |
Vacuum Filter |
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G-30 |
FilterBio |
1set |
EUR 223.5 |
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Description: Glass Funnel, 300ml |
Vacuum Filter |
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FB-1 |
FilterBio |
1set |
EUR 240.9 |
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Description: 1000ml Glass Solvent Collection |
Vacuum Filter |
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FB-2 |
FilterBio |
1set |
EUR 249.6 |
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Description: 2000ml Glass Solvent Collection |
Vacuum Filter |
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FB-G-01 |
FilterBio |
1set |
EUR 327.9 |
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Description: 1) 300ml Glass Funnel with cover 2) 1000ml Glass Solvent Collection 3) Anodized Aluminum Clamp |
Vacuum Filter |
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FB-G-02 |
FilterBio |
1set |
EUR 354 |
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Description: 1) 500ml Glass Funnel with cover 2) 2000ml Glass Solvent Collection 3) Anodized Aluminum Clamp |
Vacuum Filter |
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FB-G-30 |
FilterBio |
1set |
EUR 293.1 |
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Description: 1) Glass funnel, 300ml 2) fritted glass base 3) Clamp4) Silicone stopper |
Vacuum Filter |
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FB-G-50 |
FilterBio |
1set |
EUR 301.8 |
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Description: 1) Glass funnel, 500ml 2) fritted glass base 3) Clamp4) Silicone stopper |
Vacuum Filter |
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FB-MC-G1 |
FilterBio |
1set |
EUR 597.6 |
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Description: 1) Stainless Steel Manifold 2) One Glass Filtration Units 3) Two pieces of hosepipe |
Vacuum Filter |
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FB-MC-G3 |
FilterBio |
1set |
EUR 1067.4 |
|
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Description: 1) Stainless Steel Manifold 2) Three Glass Filtration Units 3) Two pieces of hosepipe |
Vacuum Filter |
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FB-MC-G6 |
FilterBio |
1set |
EUR 1589.4 |
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Description: 1) Stainless Steel Manifold 2) Six Glass Filtration Units 3) Two pieces of hosepipe |
Vacuum Filter |
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FB-MC-S1 |
FilterBio |
1set |
EUR 649.8 |
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Description: 1) Stainless Steel Manifold 2)One Stainless Steel Filtration Units 3) Two pieces of hosepipe |
Vacuum Filter |
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FB-MC-S3 |
FilterBio |
1set |
EUR 1119.6 |
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Description: 1) Stainless Steel Manifold 2) Three Stainless Steel Filtration Units 3) Two pieces of hosepipe |
Vacuum Filter |
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FB-MC-S6 |
FilterBio |
1set |
EUR 1659 |
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Description: 1) Stainless Steel Manifold 2) Six Stainless Steel Filtration Units 3)Two pieces of hosepipe |
 LUMITEIN PROTEIN GEL STAIN, 1X |
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21001-1 |
Biotium |
1L |
EUR 271.2 |
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Description: Minimum order quantity: 1 unit of 1L |
LUMITEIN PROTEIN GEL STAIN, 1X |
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21001-2 |
Biotium |
5L |
EUR 962.4 |
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Description: Minimum order quantity: 1 unit of 5L |
 LUMITEIN PROTEIN GEL STAIN, 100X |
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21002-1 |
Biotium |
10ML |
EUR 213.6 |
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Description: Minimum order quantity: 1 unit of 10ML |
LUMITEIN PROTEIN GEL STAIN, 100X |
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21002-2 |
Biotium |
50ML |
EUR 760.8 |
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Description: Minimum order quantity: 1 unit of 50ML |
 Maximo-Gel juice fluorescent stain |
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310012L |
GeneOn |
10x10 ml |
EUR 1904.4 |
) Gel tray V (for Visirays) |
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2195934 |
Atto |
2unit |
EUR 356.4 |
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Description: Opt i on |
) Gel tray (dams not included) |
|
2393382 |
Atto |
1unit |
EUR 295.2 |
 0.75 mm Compact Gel Cast |
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2393632 |
Atto |
2unit |
EUR 458.4 |
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Description: I ncl udi ng 1 set of gl ass pl at es and 1 comb |
 Gel Holders 2/pk, MP |
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2393726 |
Atto |
3unit |
EUR 316.8 |
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Description: For hand-cast gel s |
 Gel carrier for AE-6541 |
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2394140 |
Atto |
10unit |
EUR 303.6 |
 SDS-PAGE Gel Preparation Kit |
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abx090646-1Kit |
Abbexa |
1 Kit |
EUR 243.6 |
|
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 SYBR Safe DNA Gel Stain |
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A8743-.5 |
ApexBio |
500 ul |
EUR 142.8 |
SYBR Safe DNA Gel Stain |
|
A8743-1 |
ApexBio |
1 ml |
EUR 193.2 |
SYBR Safe DNA Gel Stain |
|
A8743-10 |
ApexBio |
10x1 ml |
EUR 838.8 |
SYBR Safe DNA Gel Stain |
|
A8743-100 |
ApexBio |
100x1 ml |
EUR 4068 |
) Gel Extraction Kit (100 preps) |
|
9K-006-0001 |
Bio Basic |
100preps |
EUR 283.42 |
|
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) Gel Extraction Kit (200 preps) |
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9K-006-0002 |
Bio Basic |
200preps, 200prep |
EUR 418.09 |
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 ISOLATE II PCR & Gel Kit |
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BIO-52058 |
Bioline |
10 preps |
Ask for price |
ISOLATE II PCR & Gel Kit |
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BIO-52059 |
Bioline |
50 preps |
Ask for price |
ISOLATE II PCR & Gel Kit |
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BIO-52060 |
Bioline |
250 preps |
Ask for price |
SDS-PAGE Gel Preparation Kit |
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AR0138 |
BosterBio |
1kit (Enough for 30-50 pieces of gel.) |
EUR 115.2 |
 gel casting tray 20x10 cm |
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EHS1400-TRAY10 |
Consort |
ea |
EUR 139.2 |
 gel casting tray 20x20 cm |
|
EHS1400-TRAY20 |
Consort |
ea |
EUR 139.2 |
 gel casting tray 20x25 cm |
|
EHS1400-TRAY25 |
Consort |
ea |
EUR 139.2 |
 gel casting tray 26x16 cm |
|
EHS1500-TRAY16 |
Consort |
ea |
EUR 219.6 |
 gel casting tray 26x24 cm |
|
EHS1500-TRAY24 |
Consort |
ea |
EUR 219.6 |
 gel casting tray 26x32 cm |
|
EHS1500-TRAY32 |
Consort |
ea |
EUR 219.6 |
 gel casting tray 7x10 cm |
|
EHS1100-TRAY10 |
Consort |
ea |
EUR 78 |
 gel casting tray 7x7 cm |
|
EHS1100-TRAY7 |
Consort |
ea |
EUR 78 |
 gel casting tray 10x10 cm |
|
EHS1200-TRAY10 |
Consort |
ea |
EUR 87.6 |
 gel casting tray 10x7 cm |
|
EHS1200-TRAY7 |
Consort |
ea |
EUR 87.6 |
 gel casting tray 15x10 cm |
|
EHS1300-TRAY10 |
Consort |
ea |
EUR 87.6 |
 gel casting tray 15x15 cm |
|
EHS1300-TRAY15 |
Consort |
ea |
EUR 87.6 |
 gel casting tray 15x7 cm |
|
EHS1300-TRAY7 |
Consort |
ea |
EUR 87.6 |
 midi gel dryer 21x31 cm |
|
EDRY1100 |
Consort |
ea |
EUR 2247.6 |
 maxi gel dryer 35x45 cm |
|
EDRY1200 |
Consort |
ea |
EUR 2958 |
 gel casting base 10 cm |
|
EVS1100-BASE |
Consort |
ea |
EUR 180 |
 mini-wide tube gel insert |
|
EVS1200-TUBEINSERT |
Consort |
ea |
EUR 524.4 |
 gel casting base 20 cm |
|
EVS1300-BASE |
Consort |
ea |
EUR 240 |
 gel casting base for evs3100 |
|
EVS3100-BASE |
Consort |
ea |
EUR 361.2 |
 gel casting base for evs3200 |
|
EVS3200-BASE |
Consort |
ea |
EUR 361.2 |
 gel casting base for evs3300 |
|
EVS3300-BASE |
Consort |
ea |
EUR 361.2 |
) Finegel Stacking Gel Solution(5%) |
|
F1505-010 |
GenDepot |
100ml |
EUR 116.4 |
) GEL Purification Mini Kit (50prep) |
|
FAGPK-001 |
Favorgen |
50 preps |
EUR 144 |
) GEL Purification Mini Kit (200prep) |
|
FAGPK-001-1 |
Favorgen |
200 preps |
EUR 202.8 |
) GEL Purification Mini Kit (300prep) |
|
FAGPK-001-2 |
Favorgen |
300 preps |
EUR 226.8 |
) MicroElute GEL Purification Kit (50prep) |
|
FAMGK-001 |
Favorgen |
50 preps |
EUR 151.2 |
) MicroElute GEL Purification Kit (200prep) |
|
FAMGK-001-1 |
Favorgen |
200 preps |
EUR 224.4 |
 SafePinky DNA Gel Staining Solution |
|
S1000-050 |
GenDepot |
500ml |
EUR 157.2 |
SafePinky DNA Gel Staining Solution |
|
S1000-100 |
GenDepot |
2X500ml |
EUR 225.6 |
SafePinky DNA Gel Staining Solution |
|
S1000-200 |
GenDepot |
4X500ml |
EUR 336 |
 iVDye 6X Gel Loading Dye |
|
V1004-001 |
GenDepot |
1ml |
EUR 87.6 |
iVDye 6X Gel Loading Dye |
|
V1004-010 |
GenDepot |
10x1ml |
EUR 213.6 |
iVDye 6X Gel Loading Dye |
|
V1004-020 |
GenDepot |
20x1ml |
EUR 258 |
 Zenoquick Gel DNA Recovery Kit |
|
Z3001-050 |
GenDepot |
50 preps |
EUR 238.8 |
Zenoquick Gel DNA Recovery Kit |
|
Z3001-200 |
GenDepot |
4x50 preps |
EUR 757.2 |
 FitAmp Gel DNA Isolation Kit |
|
P-1007 |
EpiGentek |
100 Samples |
EUR 324.48 |
 Hi-Bind? T-Gel-Agarose |
|
M1313-1 |
Biovision |
each |
EUR 157.2 |
Hi-Bind? T-Gel-Agarose |
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M1313-10 |
Biovision |
each |
EUR 261.6 |
Hi-Bind? T-Gel-Agarose |
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M1313-100 |
Biovision |
each |
EUR 1801.2 |
 SCF515 filter, 50mm |
|
2008060 |
Atto |
1unit |
EUR 382.8 |
|
Description: For AB-6935B |
 Fluorescence filter kit |
|
2009170 |
Atto |
1unit |
EUR 2469.6 |
|
Description: WU V-20M and fi l t er (SWP/YA-3/OY) |
All samples decreased murine peritoneal phagocytic activity, with λ-samples have higher efficacy than their partners; All LMWDPS stimulates the motility of peritoneum macrophages, with κ-samples that have higher efficacy than their colleagues, we have shown that κ and λ-carrageenans C. Armatus and LMWDPS they suppress fagocytosis activities from peritoneum macrophages under both in vitro and in in vivo conditions. This allows them to be seen as pharmacological active substances and manufactures the need for further investigation.
