Many outbreaks of Burkholderia Cepacia Complex (BCC) infection are associated with contamination in personal care products (PCPS). This study aims to analyze the collection of BCC isolates in PCPs and assess preservative vulnerabilities, including Dimethoxy Dimethyl Hydantoin (DMDMH), Methylisothyzolinone-Chloromethylisothiazolinone (MIT / CMIT), and Methyl 4-hydroxybenzoate (MH). BCC isolates collected during the 3-year study period (2015-2017) was further examined by biochemical identification systems, phylogenetic analysis based on the sequence of nucleotide reca, and analysis of typing multilocus sequences.
Testing of the vulnerability of BCC bacterial bacteria is evaluated by the minimum inhibition concentration and minimum bactericidal concentration. A total of seven types of different sequences (STS) were identified, which originated from four different BCC species: Burkholderia Cenocepacia (ST621, ST258, and St. Novel), Burkholderia Lata (ST339 and ST336), Burkholderia Contaminans (ST482), Burkholderia Cepacia (ST922 ).
For DMDMH and MH, the maximum concentration permitted in accordance with cosmetic safety specifications (0.6% and 0.4%) is able to inhibit or kill all BCC strains, but 40% of BCC isolates can last higher than the concentration of MIT / maximum permitted / CMIT (from the mixture in the ratio of 3: 1 of the 5-chloro-2-methyl-4-isothiazoline-3-one and 2-methyl-4-isothiazolin-3-one). PCP contamination of BCC strains must be more attention to producers because of its diversity in molecular epidemiology and low vulnerability to preservatives such as MIT / CMIT.
Find new molecular targets from natural products known in the Silico target prediction
Natural products consist of rich reservoirs for innovative drug steering and is a constant source of bioactive compounds. To find the pharmacological target for new natural products or known to use a modern computer-assisted method is the current effort in drug discovery. However, natural treasures can be used more effectively. However, a reliable pipe for predictions of large-scale targets natural products is still rare. We developed a workflow in Silico consisting of four independent target and stand-alone prediction tools and evaluated its performance on Dihydrochalcones (DHCS) -The well-known natural product. Thus, we revealed four protein targets that were previously not reported for DHC, namely 5-lipoxygenase, cyclooloxygenase-1, 17β-hydroxysteroid dehydrogenase 3, and Aldo-Keto Reductase 1C3. In addition, we provide a comprehensive strategy on how to predict computing targets and guides using each tool.
Farmacophore-based virtual playback, molecular docking, molecular dynamics and MM-GBSA approach to identification of candidates for SARS-COV-2 inhibitors from natural product databases
Covid-19 caused by severe acute respiratory syndrome Coronavirus 2 (SARS-COV-2) mainly appeared in Wuhan, China, in December 2019. At present, there is no precise therapy and vaccination available for this disease, and increasing from day to day with a high mortality rate. Farmacophore-based virtual playback of the natural product database chosen followed by Glide Molecular Docking and the dynamics of the study of the main Protease SARS-COV-2 is investigated to identify potential ligands that can act as inhibitors. The molecule <b> SN00293542 </ b> and <b> SN00382835 </ b> reveals the highest docking score <b> -14.57 </ b> and <b> -12.42 kcal / mol, </ b> if Compared to Ligand Co-Crystal PDB-6Y2F (O6K) and 6W63 (X77) from SARS-COV-2 M <Soup> Pro </ soup>.
To further validate the Top score molecular interaction <b> SN00293542 </ b> and <b> SN00382835, </ b> Molecular Dynamics Study 100 NS is done. It shows that the complex protein-ligands are stable throughout the simulation period, and minimal backbone fluctuations have occurred in the system. Post-mm-GBSA analysis of molecular dynamics data shows free fastener energy – <b> 71.7004 +/- 7.98, -56.81 +/- 7.54 kcal / mol, </ b>. Computing studies identify several ligands that can act as potential inhibitors of SARS-COV-2 M <Soup> Pro </ soup>. Top ranking molecules <b> SN00293542, </ b> and <b> SN00382835 </ b> occupying the active target site, such main proteases from co-crystal ligands. These molecules may appear as ligands that promise SARS-COV-2 and thus need further detailed investigations. Communicated by Ramaswamy H. Sarma.
Immunological effects of chondrusarmatus Carragentens and low molecular degradation products are low
High molecular weight capability (HMW) κ- and λ-carrageenans from chondrusarmatus red marine algae and low molecular weight degradation products (LMWDPS) (0.7-20 and 10-170 kDa- respectively) to influence functional properties (motility and phagocytosis ) Murine Peritoneal Macrophag WeakSessesSedin This research as anin vitro and a week’s meal experiment.
We show that, with anexception of one, all barrage samples at 100 μg / ml increase cellular motility and dosage-dependently decrease in fagocytic activity; LMWDPS from λ-Carrageenan suppresses motility and has no effect on phagocytosis. Oral administration of all carpararacten samples with 100 μg / kg / day for 7 days for MiChad has no effect on their clinical appearance, weight, their weight, spleen or spleen department of the changes seen in their inner organs. All samples from the composition of the peritoneum cavity to macrophages. The consumption of LMWDPS from κ-Carrageenan results in the construction of leukopenia, but there are no changes to the relative WBC count introduced by one of the samples.
 Gel Cutter |
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KS071012-11 |
Biochain |
12 |
EUR 89 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
) Short wave pass filter (SWP filter) |
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2130430 |
Atto |
1unit |
EUR 648 |
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Description: Shor t wave pass fi l t er 650nm |
) 96 well filter plate (no filter) |
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SD5006-EMPTY |
Bio Basic |
12UNIT |
EUR 87.41 |
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 Filter Holder |
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2006121 |
Atto |
2unit |
EUR 374 |
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Description: Opt i on |
 Vacuum Filter |
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Clamp |
FilterBio |
1set |
EUR 163.05 |
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Description: Anodized Aluminum Clamp |
Vacuum Filter |
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FB-1 |
FilterBio |
1set |
EUR 200.75 |
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Description: 1000ml Glass Solvent Collection |
Vacuum Filter |
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FB-2 |
FilterBio |
1set |
EUR 208 |
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Description: 2000ml Glass Solvent Collection |
Vacuum Filter |
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FB-G-01 |
FilterBio |
1set |
EUR 273.25 |
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Description: 1) 300ml Glass Funnel with cover 2) 1000ml Glass Solvent Collection 3) Anodized Aluminum Clamp |
Vacuum Filter |
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FB-G-02 |
FilterBio |
1set |
EUR 295 |
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Description: 1) 500ml Glass Funnel with cover 2) 2000ml Glass Solvent Collection 3) Anodized Aluminum Clamp |
Vacuum Filter |
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FB-G-30 |
FilterBio |
1set |
EUR 244.25 |
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Description: 1) Glass funnel, 300ml 2) fritted glass base 3) Clamp4) Silicone stopper |
Vacuum Filter |
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FB-G-50 |
FilterBio |
1set |
EUR 251.5 |
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Description: 1) Glass funnel, 500ml 2) fritted glass base 3) Clamp4) Silicone stopper |
Vacuum Filter |
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FB-MC-G1 |
FilterBio |
1set |
EUR 498 |
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Description: 1) Stainless Steel Manifold 2) One Glass Filtration Units 3) Two pieces of hosepipe |
Vacuum Filter |
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FB-MC-G3 |
FilterBio |
1set |
EUR 889.5 |
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Description: 1) Stainless Steel Manifold 2) Three Glass Filtration Units 3) Two pieces of hosepipe |
Vacuum Filter |
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FB-MC-G6 |
FilterBio |
1set |
EUR 1324.5 |
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Description: 1) Stainless Steel Manifold 2) Six Glass Filtration Units 3) Two pieces of hosepipe |
Vacuum Filter |
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FB-MC-S1 |
FilterBio |
1set |
EUR 541.5 |
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Description: 1) Stainless Steel Manifold 2)One Stainless Steel Filtration Units 3) Two pieces of hosepipe |
Vacuum Filter |
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FB-MC-S3 |
FilterBio |
1set |
EUR 933 |
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Description: 1) Stainless Steel Manifold 2) Three Stainless Steel Filtration Units 3) Two pieces of hosepipe |
Vacuum Filter |
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FB-MC-S6 |
FilterBio |
1set |
EUR 1382.5 |
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Description: 1) Stainless Steel Manifold 2) Six Stainless Steel Filtration Units 3)Two pieces of hosepipe |
Vacuum Filter |
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G-30 |
FilterBio |
1set |
EUR 186.25 |
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Description: Glass Funnel, 300ml |
Vacuum Filter |
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G-50 |
FilterBio |
1set |
EUR 197.85 |
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Description: Glass Funnel, 500ml |
) Ketanserin (Vulketan Gel) |
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E1KS2232 |
EnoGene |
50mg |
EUR 434 |
 Gel tray L |
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2322112 |
Atto |
5unit |
EUR 257 |
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Description: 2pcs/1set |
 Gel tray S |
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2322117 |
Atto |
5unit |
EUR 257 |
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Description: 2pcs/1set |
 Aluminium Phosphate Gel |
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VAdv-Ly0001 |
Creative Biolabs |
250 mL |
EUR 1386 |
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Description: Aluminium Phosphate Gel 2%, Aluminum salt Vaccine adjuvant. |
 Aluminium Hydroxide Gel |
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VAdv-Ly0003 |
Creative Biolabs |
250 mL |
EUR 1386 |
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Description: Aluminium Hydroxide Gel 2%, Aluminum salt Vaccine adjuvant. |
 ViSafe RED Gel |
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S430 |
GeneOn |
500 µl |
EUR 146 |
ViSafe RED Gel |
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S430L |
GeneOn |
5x500 µl |
EUR 557 |
 ViSafe Green Gel |
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S435 |
GeneOn |
500 µl |
EUR 146 |
ViSafe Green Gel |
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S435L |
GeneOn |
5x500 µl |
EUR 557 |
 Gel Breaker Tubes |
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KS071012-12 |
Biochain |
12 |
EUR 89 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 SCF515 filter, 50mm |
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2008060 |
Atto |
1unit |
EUR 319 |
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Description: For AB-6935B |
 Fluorescence filter kit |
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2009170 |
Atto |
1unit |
EUR 2058 |
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Description: WU V-20M and fi l t er (SWP/YA-3/OY) |
 mini tube gel insert |
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EVS1100-TUBEINSERT |
Consort |
ea |
EUR 411 |
 maxi tube gel insert |
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EVS1300-TUBEINSERT |
Consort |
ea |
EUR 504 |
 dam for 15cm gel |
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EHS3400-DAM |
Consort |
ea |
EUR 158 |
 dam for 20cm gel |
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EHS3500-DAM |
Consort |
ea |
EUR 166 |
 dam for 23.5cm gel |
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EHS3600-DAM |
Consort |
ea |
EUR 166 |
 Gel DNA Recovery Kit |
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GP05 |
GeneOn |
5 preps |
Ask for price |
Gel DNA Recovery Kit |
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GP100 |
GeneOn |
100 preps |
EUR 130 |
Gel DNA Recovery Kit |
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GP200 |
GeneOn |
200 preps |
EUR 189 |
Gel DNA Recovery Kit |
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GP50 |
GeneOn |
50 preps |
EUR 94 |
) AmbiClean Kit (PCR & Gel) |
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GV05 |
GeneOn |
5 preps |
Ask for price |
AmbiClean Kit (PCR & Gel) |
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GV100 |
GeneOn |
100 preps |
EUR 128 |
AmbiClean Kit (PCR & Gel) |
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GV200 |
GeneOn |
200 preps |
EUR 189 |
AmbiClean Kit (PCR & Gel) |
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GV50 |
GeneOn |
50 preps |
EUR 91 |
 Drying Reagent, Silica Gel |
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DSG110 |
Bio Basic |
1Kg |
EUR 76.1 |
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 Gel casting stand L |
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2322111 |
Atto |
5unit |
EUR 257 |
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Description: 1pc |
 Gel casting stand S |
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2322116 |
Atto |
5unit |
EUR 257 |
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Description: 1pc |
 Quick Gel Extraction Kit |
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20-abx098085 |
Abbexa |
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 Agarose, Low melting gel |
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AB0015 |
Bio Basic |
10g |
EUR 80.45 |
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 SensitiveSafe DNA Gel Stain |
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W139-500 |
101Bio |
- |
Ask for price |
 10x Gel Loading Buffer |
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TG4038 |
TopoGen |
1ml |
EUR 156 |
 ProsiBlue Gel Staining Solution |
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P7300-050 |
GenDepot |
500ml |
EUR 174 |
 Anti-Flag Affinity Gel |
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B23101 |
Bimake |
1 ml Kit |
EUR 483 |
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Description: Anti-Flag Affinity Gel is a Protein A purIFied murine IgG2b Monoclonal antibody covalently attached to sepharose 4B wHICh could used on Flag fusion protein purIFication, IP and CO-IP. |
Anti-Flag Affinity Gel |
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B23102 |
Bimake |
5 ml Kit |
EUR 1452 |
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Description: Anti-Flag Affinity Gel is a Protein A purIFied murine IgG2b Monoclonal antibody covalently attached to sepharose 4B wHICh could used on Flag fusion protein purIFication, IP and CO-IP. |
) OPTIONAL BLUE FILTER (497NM) |
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GDBL-BLUE |
CORNING |
1/pk |
EUR 1082 |
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Description: Lab Equipment; Axygen Branded EQ |
) OPTIONAL GREEN FILTER (572NM) |
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GDBL-GREEN |
CORNING |
1/pk |
EUR 1052 |
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Description: Lab Equipment; Axygen Branded EQ |
 R-60 filter, 50mm |
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2130452 |
Atto |
2unit |
EUR 301 |
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Description: For AE-6935GL |
 HYDROPHOBIC PTFE FILTER 0,2µM |
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4991 |
CORNING |
1/pk |
EUR 61 |
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Description: Handheld Pipettor Repairs; Liquid Handling Device Repairs |
 HYDROPHOBIC PTFE FILTER 0,45µM |
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4992 |
CORNING |
1/pk |
EUR 61 |
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Description: Handheld Pipettor Repairs; Liquid Handling Device Repairs |
 SYRINGE FILTER,50MM,0.2UM,PTFE,VENT FILTER,S,IND,1/12 |
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431227 |
CORNING |
1/pk |
EUR 140 |
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Description: Lab Filtration; Corning Syringe Filters |
) GEL Purification Mini Kit (50prep) |
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FAGPK-001 |
Favorgen |
50 preps |
EUR 120 |
) GEL Purification Mini Kit (200prep) |
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FAGPK-001-1 |
Favorgen |
200 preps |
EUR 169 |
) GEL Purification Mini Kit (300prep) |
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FAGPK-001-2 |
Favorgen |
300 preps |
EUR 189 |
) MicroElute GEL Purification Kit (50prep) |
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FAMGK-001 |
Favorgen |
50 preps |
EUR 126 |
) MicroElute GEL Purification Kit (200prep) |
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FAMGK-001-1 |
Favorgen |
200 preps |
EUR 187 |
 gel casting base 10 cm |
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EVS1100-BASE |
Consort |
ea |
EUR 150 |
 mini-wide tube gel insert |
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EVS1200-TUBEINSERT |
Consort |
ea |
EUR 437 |
 gel casting base 20 cm |
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EVS1300-BASE |
Consort |
ea |
EUR 200 |
 gel casting base for evs3100 |
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EVS3100-BASE |
Consort |
ea |
EUR 301 |
 gel casting base for evs3200 |
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EVS3200-BASE |
Consort |
ea |
EUR 301 |
 gel casting base for evs3300 |
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EVS3300-BASE |
Consort |
ea |
EUR 301 |
 SDS-PAGE Gel Preparation Kit |
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AR0138 |
BosterBio |
1kit (Enough for 30-50 pieces of gel.) |
EUR 96 |
 gel casting tray 15x10 cm |
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EHS1300-TRAY10 |
Consort |
ea |
EUR 73 |
All samples decreased murine peritoneal phagocytic activity, with λ-samples have higher efficacy than their partners; All LMWDPS stimulates the motility of peritoneum macrophages, with κ-samples that have higher efficacy than their colleagues, we have shown that κ and λ-carrageenans C. Armatus and LMWDPS they suppress fagocytosis activities from peritoneum macrophages under both in vitro and in in vivo conditions. This allows them to be seen as pharmacological active substances and manufactures the need for further investigation.
