Natural products, which can be isolated from living organisms around the world, have played an important role in drug discovery since ancient times. However, it has become more challenging to identify novel molecules structurally with promising biological activities for pharmaceutical development, especially because the methodology is limited to their acquisition. In this review, we summarize our research recently that activates the potential biosynthesis of fungal filaments by genetic engineering to utilize the flow of metabolism for natural product production that has never happened before.
The recent revolution in the genome sequencing technology allows accumulated a large amount of information about biosynthetic genes, molecular construction blueprint. Utilizing the specified heterologist expression system, activation of specific path transcription factors coupled with the knockout system strategy, and manipulating the global regulatory gene, biosintetic genes are exploited to activate the biosynthetic pathways and describe encoded enzyme functions. We show that this methodology is useful for acquiring fungal assets for drug discovery. These studies also allow the investigation of the molecular function of natural products in the development of fungi.
Virtual screening and molecular simulation study database natural products for lead identification novel novel coronavirus protease inhibitors
3CL LIKE PROTEASE (3CLPRO or MPRO) is one of the main proteases of 2019-Ncov. 3CLPRO is a nontructural protein of SARS-COV and has an important role in viral replication and transcription, thus, can be a potential target for the development of anti-SARS drugs. This study employs a ligand and structural-based approach to identify the effective inhibitor of Protease 2019-NCOV. E-pharmacophore developed from a virtual hit produced by 3CLPro-1, which is worn through the possibility of drugs and filter pain to eliminate disturbing compounds.
A further comprehensive docking study, free energy calculations and admation studies produce two lead virtual-molport-000-410-348 and molport-002-530-156. MOLPORT-000-410-348 and MOLPORT-002-530-156 compounds display good docking scores from -12.09 and -13.38 kcal / mol and free binder energy -63.34 ± 2.03 and -61.52 ± 2.24 kcal / mole. Compounds also showcase Predike Profile predictions and subject to Dynamic Molecular Studies (MD). MD simulation produces a stable complex of these ligands with 3CLPRO proteins and RMSD ligands within acceptable limits. Communicated by Ramaswamy H. Sarma.
Relationship between activities, molecular docking, and molecular dynamic studies from expending from marine natural products with anti-HIV activity
HIV-1 infection is a global epidemic whose treatment is limited to the majority by viral resistance and side effects. Natural products from algae have been studied for years, including antivirus, becoming an alternative to the design of anti-HIV drugs. Because natural product insulation can be in the form of obstacles, molecular modeling is an important tool for studying these compounds. Here, relationships of activities, molecular docking, and molecular dynamic studies are carried out to direct the study of ten natural marine products with anti-HIV activity.

In the relationship between activities, the descriptor is identified associating the activity of anti-HIV five by passing with possible actions on the Alostalister transcriptase site back. Diarpene was evaluated by molecular docking, and was identified that only dolabelladirioletririol was interacting on the alosteric site. Molecular dynamics suggest that dolabelladienetririol can disrupt the RNA virus that binds for HIV-1 RT by inducing changes in enzyme conformation. Also, in Silico’s simulation, predict that the dolabelladiriol presents high
Molecular genome features related to in vitro responses from NCI-60 cancer cell line panels to natural products
Natural products remain a significant source of anti-cancer chemotherapy. Searching for drugs targeted for cancer treatment includes consideration of natural products that can provide new opportunities for anti-tumor cytotoxicity as a single agent or in combination therapy. We examine the association of molecular genome features in the NCI-60 cancer cell line panel well with in vitro responses to treatment with 1,302 small molecules that include natural products, semi-synthetic natural products, and synthetic compounds based on natural products Farmacophore from the therapy program Progression of national national cancer institute database. These compounds are obtained from various plant, sea and microbial species.
Molecular information used for analysis including expression size for 23,059 annotation transcripts, LNCRNAS, and MIRNAS, and data on a single nucleotide variant that changes protein in 211 genes related to cancer. We found the associations of various genes including SLFN11, CYP2J2, EFX1, GPC1, ELF3, and MGMT involved in improving DNA damage, family members of notch, ABC and SLC Transporter, and both mutations in Tyrosine and Braf V600e kinases with NCI response with NCI -60 for the specific category of natural products. Hierarchical clustering identifies a group of natural products that correlate with certain action mechanisms.
In particular, some natural product clusters are associated with the expression of the SLFN11 gene, indicating that the potential actions of this compound can involve damage to DNA. The association between gene expression or changes in genome genomes is relevant functionally with cancer cell responses to natural products providing new information about the potential mechanism of action from a group of potential biological compounds. This information will help the discovery of drugs in the future and in the design of new cancer chemotherapy agents targeted.
A new approach based on the polyethylene form of multi-ultra high molecular weight glued to make bone replacement products
Three types of glue based on Tiol-ENE reactions, polyvinyl alcohol (PVA) / cellulose, and phenol formaldehyde is prepared and applied to the weight of the modified molecular weight polyethylene weight (UHMWPE) grafted by cellulose. Compared to unmodified UHMWPE samples, the T-Peel test in the modified UHMWPE film and grafted shows an increase in the value of skin strength for glue based on the reaction of Tiol-ENE, PVA / cellulose, and formaldehyde phenol by 40, 29, and 41 times, each each. The maximum peeled strength value of 0.62 kg / cm is obtained so that glue based on formaldehyde phenol.
Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2 |
G2007-500 |
GenDepot |
5Kg |
EUR 1751 |
Silica Gel In Bag, 5G, 100/Pk |
DSG111 |
Bio Basic |
1PK, 100UNIT |
EUR 61.31 |
|
FITC-PEG-Alkyne,400 |
FL044007-400-100mg |
Biochempeg |
100mg |
EUR 568 |
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
FA-PEG-NHS,400 |
HE057023-400-100mg |
Biochempeg |
100mg |
EUR 377 |
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
OH-PEG-OH,400 |
HO002002-400-10g |
Biochempeg |
10g |
EUR 306 |
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
Iodopolystyrene resin (200-400 mesh, > 1 mmol/g) |
D-2260.0001 |
Bachem |
1.0g |
EUR 176 |
Iodopolystyrene resin (200-400 mesh, > 1 mmol/g) |
D-2260.0005 |
Bachem |
5.0g |
EUR 611 |
4-(Fmoc-hydrazino)-benzoylaminomethyl resin (200-400 mesh) |
D-2560.0001 |
Bachem |
1.0g |
EUR 200 |
4-(Fmoc-hydrazino)-benzoylaminomethyl resin (200-400 mesh) |
D-2560.0005 |
Bachem |
5.0g |
EUR 696 |
4-Formyl-phenyloxymethyl polystyrene resin (200-400 mesh) |
D-2575.0001 |
Bachem |
1.0g |
EUR 139 |
4-Formyl-phenyloxymethyl polystyrene resin (200-400 mesh) |
D-2575.0005 |
Bachem |
5.0g |
EUR 477 |
TIPS GEL,200UL,RD,NS,RK,200/400 |
4853 |
CORNING |
200/pk |
EUR 87 |
Description: Non Filter Tips; Costar Specialty Tips |
TIPS GEL,200UL,FT,NS,RK,200/400 |
4854 |
CORNING |
200/pk |
EUR 132 |
Description: Non Filter Tips; Costar Specialty Tips |
TIPS GEL,200UL,FT,NS,RK,200/400 |
4884 |
CORNING |
200/pk |
EUR 158 |
Description: Non Filter Tips; Costar Specialty Tips |
Aminomethyl resin (200-400 mesh, 0.80-1.20 mmol/g) |
D-1005.0005 |
Bachem |
5.0g |
EUR 115 |
Description: CAS# [78578-28-6] |
Aminomethyl resin (200-400 mesh, 0.80-1.20 mmol/g) |
D-1005.0025 |
Bachem |
25.0g |
EUR 309 |
Description: CAS# [78578-28-6] |
Aminomethyl resin (200-400 mesh, 0.80-1.20 mmol/g) |
D-1005.0100 |
Bachem |
100.0g |
EUR 841 |
Description: CAS# [78578-28-6] |
Hydroxymethyl resin (200-400 mesh, 0.9-1.4 mmol/g) |
D-1160.0025 |
Bachem |
25.0g |
EUR 284 |
Description: CAS# [66072-40-0] |
Hydroxymethyl resin (200-400 mesh, 0.9-1.4 mmol/g) |
D-1160.0100 |
Bachem |
100.0g |
EUR 792 |
Description: CAS# [66072-40-0] |
Merrifield resin (200-400 mesh, 0.80-1.40 mmol/g) |
D-1245.0025 |
Bachem |
25.0g |
EUR 151 |
Description: CAS# [55844-94-5] |
Merrifield resin (200-400 mesh, 0.80-1.40 mmol/g) |
D-1245.0100 |
Bachem |
100.0g |
EUR 393 |
Description: CAS# [55844-94-5] |
Wang resin (200-400 mesh, 0.80-1.20 mmol/g) |
D-1250.0005 |
Bachem |
5.0g |
EUR 115 |
Wang resin (200-400 mesh, 0.80-1.20 mmol/g) |
D-1250.0025 |
Bachem |
25.0g |
EUR 321 |
Wang resin (200-400 mesh, 0.80-1.20 mmol/g) |
D-1250.0100 |
Bachem |
100.0g |
EUR 914 |
SASRIN resin (200-400 mesh, 0.80-1.20 mmol/g) |
D-1295.0001 |
Bachem |
1.0g |
EUR 162 |
Description: CAS# [124760-64-1] |
SASRIN resin (200-400 mesh, 0.80-1.20 mmol/g) |
D-1295.0005 |
Bachem |
5.0g |
EUR 539 |
Description: CAS# [124760-64-1] |
SASRIN resin (200-400 mesh, 0.80-1.20 mmol/g) |
D-1295.0025 |
Bachem |
25.0g |
EUR 2061 |
Description: CAS# [124760-64-1] |
PAL resin (200-400 mesh, 0.2-0.5 mmol/g) |
D-2125.0001 |
Bachem |
1.0g |
EUR 225 |
PAL resin (200-400 mesh, 0.2-0.5 mmol/g) |
D-2125.0005 |
Bachem |
5.0g |
EUR 803 |
Isocyanomethyl resin (200-400 mesh, 0.7-1.0 mmol/g) |
D-2225.0001 |
Bachem |
1.0g |
EUR 200 |
Isocyanomethyl resin (200-400 mesh, 0.7-1.0 mmol/g) |
D-2225.0005 |
Bachem |
5.0g |
EUR 696 |
Diphenyldiazomethane resin (200-400 mesh, 0.7-1.3 mmol/g) |
D-2230.0001 |
Bachem |
1.0g |
EUR 284 |
Diphenyldiazomethane resin (200-400 mesh, 0.7-1.3 mmol/g) |
D-2230.0005 |
Bachem |
5.0g |
EUR 1034 |
Thiomethyl resin (200-400 mesh, 0.9-1.2 mmol/g) |
D-2235.0001 |
Bachem |
1.0g |
EUR 200 |
Thiomethyl resin (200-400 mesh, 0.9-1.2 mmol/g) |
D-2235.0005 |
Bachem |
5.0g |
EUR 696 |
TIPS GEL-G,10UL,FT,NS,RK,200/400 |
4815 |
CORNING |
200/pk |
EUR 139 |
Description: Non Filter Tips; Costar Specialty Tips |
Benzhydrylamine resin (200-400 mesh, 0.60-0.90 mmol/g) · HCl |
D-1010.0005 |
Bachem |
5.0g |
EUR 115 |
Benzhydrylamine resin (200-400 mesh, 0.60-0.90 mmol/g) · HCl |
D-1010.0025 |
Bachem |
25.0g |
EUR 284 |
Benzhydrylamine resin (200-400 mesh, 0.60-0.90 mmol/g) · HCl |
D-1010.0100 |
Bachem |
100.0g |
EUR 780 |
Xanthenyl linker resin (200-400 mesh, 0.4-0.7 mmol/g) |
D-2040.0001 |
Bachem |
1.0g |
EUR 200 |
Xanthenyl linker resin (200-400 mesh, 0.4-0.7 mmol/g) |
D-2040.0005 |
Bachem |
5.0g |
EUR 696 |
Xanthenyl linker resin (200-400 mesh, 0.4-0.7 mmol/g) |
D-2040.0025 |
Bachem |
25.0g |
EUR 2690 |
3-Maleimidopropionylaminomethyl resin (200-400 mesh, 0.7-1.0 mmol/g) |
D-2245.0001 |
Bachem |
1.0g |
EUR 309 |
3-Maleimidopropionylaminomethyl resin (200-400 mesh, 0.7-1.0 mmol/g) |
D-2245.0005 |
Bachem |
5.0g |
EUR 1143 |
SASRIN resin carbazate (200-400 mesh, 0.6-1.2 mmol/g) |
D-2285.0001 |
Bachem |
1.0g |
EUR 284 |
SASRIN resin carbazate (200-400 mesh, 0.6-1.2 mmol/g) |
D-2285.0005 |
Bachem |
5.0g |
EUR 1034 |
Hydroxylamine-Wang resin (200-400 mesh, 0.8-1.1 mmol/g) |
D-2415.0001 |
Bachem |
1.0g |
EUR 225 |
Hydroxylamine-Wang resin (200-400 mesh, 0.8-1.1 mmol/g) |
D-2415.0005 |
Bachem |
5.0g |
EUR 792 |
4-Piperidylacetylaminomethyl resin (200-400 mesh, 0.3-0.6 mmol/g) |
D-2430.0001 |
Bachem |
1.0g |
EUR 200 |
4-Piperidylacetylaminomethyl resin (200-400 mesh, 0.3-0.6 mmol/g) |
D-2430.0005 |
Bachem |
5.0g |
EUR 696 |
4-Piperidylformylaminomethyl resin (200-400 mesh, 0.3-0.6 mmol/g) |
D-2455.0001 |
Bachem |
1.0g |
EUR 139 |
4-Piperidylformylaminomethyl resin (200-400 mesh, 0.3-0.6 mmol/g) |
D-2455.0005 |
Bachem |
5.0g |
EUR 477 |
4-Formyl-3-methoxy-phenyloxymethyl polystyrene resin (200-400 mesh) |
D-2570.0001 |
Bachem |
1.0g |
EUR 126 |
4-Formyl-3-methoxy-phenyloxymethyl polystyrene resin (200-400 mesh) |
D-2570.0005 |
Bachem |
5.0g |
EUR 418 |
DiagNano Gold Nanorods, diameter 10 nm, absorption max 700 nm |
BR-10-700 |
Creative Diagnostics |
25 mL |
EUR 585 |
DiagNano Gold Nanorods, diameter 50 nm, absorption max 700 nm |
BR-50-700 |
Creative Diagnostics |
25 mL |
EUR 720 |
DiagNano Organic Gold Nanorods, diameter 10 nm, absorption max 700 nm |
OR-10-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Organic Gold Nanorods, diameter 25 nm, absorption max 700 nm |
OR-25-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Organic Gold Nanorods, diameter 40 nm, absorption max 700 nm |
OR-40-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Organic Gold Nanorods, diameter 50 nm, absorption max 700 nm |
OR-50-700 |
Creative Diagnostics |
1mL |
EUR 1022 |
DiagNano Amine Gold Nanorods, diameter 10 nm, absorption max 700 nm |
RFA-10-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Amine Gold Nanorods, diameter 25 nm, absorption max 700 nm |
RFA-25-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Amine Gold Nanorods, diameter 40 nm, absorption max 700 nm |
RFA-40-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Amine Gold Nanorods, diameter 50 nm, absorption max 700 nm |
RFA-50-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Biotin Gold Nanorods, diameter 10 nm, absorption max 700 nm |
RFB-10-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Biotin Gold Nanorods, diameter 25 nm, absorption max 700 nm |
RFB-25-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Carboxyl Gold Nanorods, diameter 40 nm, absorption max 700 nm |
RFC-40-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Carboxyl Gold Nanorods, diameter 50 nm, absorption max 700 nm |
RFC-50-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Methyl Gold Nanorods, diameter 10 nm, absorption max 700 nm |
RFM-10-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Methyl Gold Nanorods, diameter 25 nm, absorption max 700 nm |
RFM-25-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Methyl Gold Nanorods, diameter 40 nm, absorption max 700 nm |
RFM-40-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano Methyl Gold Nanorods, diameter 50 nm, absorption max 700 nm |
RFM-50-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano NHS Gold Nanorods, diameter 25 nm, absorption max 700 nm |
RFN-25-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano NHS Gold Nanorods, diameter 40 nm, absorption max 700 nm |
RFN-40-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
DiagNano NHS Gold Nanorods, diameter 50 nm, absorption max 700 nm |
RFN-50-700 |
Creative Diagnostics |
1 mL |
EUR 1022 |
Fmoc-Ala-Wang resin (200-400 mesh, 0.40-1.00 mmol/g) |
D-1045.0005 |
Bachem |
5.0g |
EUR 187 |
Fmoc-Ala-Wang resin (200-400 mesh, 0.40-1.00 mmol/g) |
D-1045.0025 |
Bachem |
25.0g |
EUR 660 |
Fmoc-Gly-Wang resin (200-400 mesh, 0.5-0.8 mmol/g) |
D-1105.0005 |
Bachem |
5.0g |
EUR 200 |
Fmoc-Gly-Wang resin (200-400 mesh, 0.5-0.8 mmol/g) |
D-1105.0025 |
Bachem |
25.0g |
EUR 708 |
Fmoc-Leu-Wang resin (200-400 mesh, 0.50-1.00 mmol/g) |
D-1115.0005 |
Bachem |
5.0g |
EUR 187 |
Fmoc-Leu-Wang resin (200-400 mesh, 0.50-1.00 mmol/g) |
D-1115.0025 |
Bachem |
25.0g |
EUR 660 |
Fmoc-Met-Wang resin (200-400 mesh, 0.50-1.10 mmol/g) |
D-1120.0005 |
Bachem |
5.0g |
EUR 187 |
Mechanical tests for samples of multi-uhmwpe cylinder shapes, made of porous uhmwpe as a trabecular layer and armored layer (cortical layer) consisting of bulk films and UHMWPE, shows an increase in the mechanical properties of all types of glue, as a result of the existence of the UHMWPE film and Increased number of layers. The strength of the maximum press yield and modulus value of the press for the armored layer (bulk and six layers of UHMWPE film using glue based on the Tiol-ENE reaction) is 44.1 MPa (a 1730 mpa increase (increasing 36%), compared to one layer uhmwpe steel Joint reconstruction.