Natural products down plants against pseudomonas aeruginosa and staphylococcus aureus: antibiophilm activities and molecular mechanisms

Bacteria are social organisms that are able to build complex structures, such as biofilms, namely a very organized surface microorganism community, wrapped in its own extracellular matrix. Biofilms are usually related to many health problems because their formation increases resistance to antibiotics and antimicrobial agents, such as in the case of pseudomonas aeruginosa and staphylococcus aureus, two human pathogens that cause the main concern. P. Aeruginosa is responsible for severe nosocomial infections, the most often includes ventilator related pneumonia, while S. aureus causes several problems, such as skin infections, septic arthritis, and endocarditis, for only a few.

Literary data shows that natural products from plants, bacteria, fungi, and marine organisms have been shown to be effective as an anti-biofilm agent, inhibiting the formation of polymer matrices, suppressing cell adhesion and attachment, and reducing the production of virulence factors, thus blocking the quorum sensing network , Here, we focus on plant derivative chemicals, and provide literature reviews that are updated on the nature of anti-biofilms, flavonoids, alkaloids, and phenolic compounds. In addition, every time information is available, we also report the mechanism of action.

Bioactive Chrysin and Luteolin flavonoid protection measures on the formation of glyoxal of the final product of advanced gliking and human serum albumin aggregation: in vitro and molecular docking analysis

Modification of post-translational proteins by nonenzymatic (neg) and age accumulation are two underlying factors associated with long-term pathogenesis in diabetes. Glyoxal (GO) is an intermediary reactive that has the ability to modify proteins and produce age at a faster level. Human Serum Albumin (HSA) becomes the most abundant serum protein has a higher opportunity to be modified by the neg. The main objective of this study is to investigate the potential of Chrysin and Luteolin as an antigry and antifibrillation agent in mediated glication and the formation of HSA mediated. The formation of age is confirmed from spectral measurement of absorption and fluorescence.

Both flavonoids are able to satisfy the intensity of fluorescence in age in vitro indicating antiglica properties of molecules. Fibril formation in the modified HSA is confirmed by Thioflavin T (ENT) and flavonoids fluorescence tests found to provide antifibrillation properties in vitro. The results of the docking suggest that both flavonoids interact with various amino acid residues of iia subdomain, including licens are vulnerable to glication and arginine through non-covalent strength and further stabilize the HSA structure, which further explains their action mechanism as an antiglicate agent and antifibrillation.

Comprehensive quality evaluation of Fuzi and its processed products through UPLC-QTOF / MS integration combined MS / MS based spectral molecular networks with multivariate statistical analysis and HPLC-MS / MS.

Aconiti Lateralis Radix Praeparata (Chinese name is Fuzi, FZ), lateral root or son of asonitum Carmichaelii debx. (Ranunculaceae), is a controversial traditional Chinese medicine (TCM) distributed universally and applied in many countries, such as China, Japan, Korea and India. FZ can be used to treat various diseases, including rheumatic fever, rheumatism, painful joints, syncope, collapse, bronchial asthma, some endocrinal disorders, etc. However, the quality control and assessment of FZ challenges due to clear and high toxicological risks, and only processed products are permitted clinically used in accordance with relative safety regulations. As a result, it is necessary to analyze all chemical compositions and dynamic FZ changes before and after processing. Overcoming chemical changes from raw and processed products is a way to reduce toxicity.

The purpose of this study: In this article, the entire Chemical Composition of FZ was analyzed, the difference between FZ RAW and processed was evaluated, and the possibility of factors that influenced the decrease in processed FZ toxicity described from the perspective of its chemical composition using qualitative and quantitative analysis methods.

Materials and Methods: New strategies of many data collection and processing based on ultra-performance liquid chromatography coupled with the Mass Spectrometry method for quadrupole time (UPLC-QTOF / MS) in positive ion mode, along with the natural product of the global social molecular network (GNP) and analysis Multivariate Statistics, was established to systematically identify the chemical constituents of FZ and investigate comprehensively chemical markers that can be used to distinguish FZ processed with vinegar and honey from raw products. Combined with the results of qualitative analysis, 12 components, including 8 chemical markers compounds and 4 toxicity components, are analyzed quantitatively by using high-performance liquid chromatography equipped with triple-quadrupole mass spectrometry (HPLC-MS).

Agarose
G060-1	ABM	100 g	EUR 211.2

Agarose
G060-2	ABM	500 g	EUR 442.8

Agarose
Z5050007	Biochain	500 g	EUR 738
Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time.

Agarose
Z5050013	Biochain	100 g	EUR 226.8
Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time.

Fish Thyroxine,T5 ELISA Kit
CN-00301F2	ChemNorm	48T	EUR 364.8

Rat thyroxine,T5 ELISA Kit
CN-01989R2	ChemNorm	48T	EUR 368.4

?-1,4-Gal-T5 Polyclonal Antibody
ES3750-100ul	ELK Biotech	100ul	EUR 334.8
Description: A Rabbit Polyclonal antibody against ?-1,4-Gal-T5 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

?-1,4-Gal-T5 Polyclonal Antibody
ES3750-50ul	ELK Biotech	50ul	EUR 248.4
Description: A Rabbit Polyclonal antibody against ?-1,4-Gal-T5 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

Anti- beta-1,4-Gal-T5 Antibody
A09370	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for beta-1,4-Gal-T5 Antibody (B4GALT5) detection. Tested with WB in Human, Mouse.

GalNAc-T5, soluble fragment, Human Recombinant
P1217-5	Biovision		EUR 288

anti-R-PTP Mu (T5-AF1A8)
LF-MA0354	Abfrontier	100 ul	EUR 400.8
Description: Mouse monoclonal to R-PTP Mu

Anti-beta-1,4-Gal-T5 antibody
STJ96375	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to beta-1,4-Gal-T5.

Agarose, Sepro
A0224-010	GenDepot	100g	EUR 262.8

Agarose, Sepro
A0224-020	GenDepot	200g	EUR 343.2

Agarose, Sepro
A0224-050	GenDepot	500g	EUR 522

Agarose L.M.P
20-abx082386	Abbexa	EUR 326.40 EUR 260.40	10 g 5 g

Agarose M
AB0013	Bio Basic	50g	EUR 91.32

Agarose LE
A-1270-100	ABTBeads	100 g	EUR 139.2

Agarose LE
A-1270-500	ABTBeads	500 g	EUR 349.2

Agarose HR
A-1280-100	ABTBeads	100 g	EUR 219.6

Agarose HR
A-1280-500	ABTBeads	500 g	EUR 822

Agarose GA
A-1290-100	ABTBeads	100 g	EUR 123.6

Agarose GA
A-1290-500	ABTBeads	500 g	EUR 306

Agarose LM
A-1300-100	ABTBeads	100 g	EUR 230.4

Agarose LM
A-1300-25	ABTBeads	25 g	EUR 123.6

Agarose Tablets
BA50201	Bioatlas	100pcs	EUR 130.8
Description: Ultrapure agarose for separation and further analysis of PCR products.

Agarose A
D0012	Bio Basic	50g	EUR 96.54

Agarose StandardRoomTemperature
FYA002-100G	Yeastern Biotech	100 g	Ask for price

Agarose StandardRoomTemperature
FYA002-500G	Yeastern Biotech	500 g	Ask for price

Beta-1, 4-Gal-T5 Polyclonal Antibody
ABP52751-003ml	Abbkine	0.03ml	EUR 189.6
Description: A polyclonal antibody for detection of Beta-1, 4-Gal-T5 from Human, Mouse. This Beta-1, 4-Gal-T5 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human ?-1,4-Gal-T5 at AA range: 290-370

Beta-1, 4-Gal-T5 Polyclonal Antibody
ABP52751-01ml	Abbkine	0.1ml	EUR 346.8
Description: A polyclonal antibody for detection of Beta-1, 4-Gal-T5 from Human, Mouse. This Beta-1, 4-Gal-T5 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human ?-1,4-Gal-T5 at AA range: 290-370

Beta-1, 4-Gal-T5 Polyclonal Antibody
ABP52751-02ml	Abbkine	0.2ml	EUR 496.8
Description: A polyclonal antibody for detection of Beta-1, 4-Gal-T5 from Human, Mouse. This Beta-1, 4-Gal-T5 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human ?-1,4-Gal-T5 at AA range: 290-370

Rat Neonatal Thyroxine,NN-T5 ELISA Kit
CN-01992R2	ChemNorm	48T	EUR 405.6

Protein A-Agarose
6526-1	Biovision		EUR 183.6

Protein A-Agarose
6526-100	Biovision		EUR 3550.8

Protein A-Agarose
6526-1000	Biovision		Ask for price

Protein A-Agarose
6526-25	Biovision		EUR 1410

Protein A-Agarose
6526-5	Biovision		EUR 502.8

Nickel Agarose Extrachel
6RREXCH-100	ABTBeads	100 ml	EUR 1263.6

Nickel Agarose Extrachel
6RREXCH-25	ABTBeads	25 ml	EUR 380.4

Nickel Agarose Extrachel
6RREXCH-500	ABTBeads	500 ml	EUR 4698

HiQTM Standard Agarose
A0222-020	GenDepot	200g	EUR 300

HiQTM Standard Agarose
A0222-050	GenDepot	500g	EUR 537.6

Glutathione Agarose Resin
4B-GLU-10	ABTBeads	10 ml	EUR 207.6

Glutathione Agarose Resin
4B-GLU-100	ABTBeads	100 ml	EUR 1195.2

Biotin Agarose Resin
4BCL-BI-10	ABTBeads	10 ml	EUR 493.2

Biotin Agarose Resin
4BCL-BI-5	ABTBeads	5 ml	EUR 282

GelRed Agarose LE
41029-50G	Biotium	50G	EUR 384
Description: Minimum order quantity: 1 unit of 50G

GelRed Agarose LE
41029-5G	Biotium	5G	EUR 124.8
Description: Minimum order quantity: 1 unit of 5G

GelGreen Agarose LE
41030-50G	Biotium	50G	EUR 384
Description: Minimum order quantity: 1 unit of 50G

GelGreen Agarose LE
41030-5G	Biotium	5G	EUR 124.8
Description: Minimum order quantity: 1 unit of 5G

Agarose GPG/LE
A2500-100000	ApexBio	100 g	EUR 170.4
Description: Agar is a galactan polysaccharides which extracted from the intracellular matrix in marine algae Rhodophyta. Agarose is a neutral polymer component of agar. Agarose solution transforms into gel after heating. Gel is formed resulting from double helical formation from molecular chains.

Agarose GPG/LE
A2500-500000	ApexBio	500 g	EUR 560.4
Description: Agar is a galactan polysaccharides which extracted from the intracellular matrix in marine algae Rhodophyta. Agarose is a neutral polymer component of agar. Agarose solution transforms into gel after heating. Gel is formed resulting from double helical formation from molecular chains.

Agarose GPG/ME
A2502-100000	ApexBio	100 g	EUR 226.8
Description: analytical and preparative electrophoresis of DNA >1000bpnorthern/southern blottingserum protein electrophoresis.

Agarose GPG/ME
A2502-500000	ApexBio	500 g	EUR 811.2
Description: analytical and preparative electrophoresis of DNA >1000bpnorthern/southern blottingserum protein electrophoresis.

Agarose, Resolute GPG
A2503-100000	ApexBio	100 g	EUR 477.6
Description: resolve differences in bp length by as little as 2%finely resolve DNA fragments from 20-1000bp, rivals polyacrylamidegels are extremely clear and easy to handle due to high gel strengthno detectable inhibition of DNA ligase or the following restriction enzymes:BamH I, Hind II, EcoR I..

Benchmark Agarose LE
A1700	Benchmark Scientific	1 PC	EUR 385.98

Benchmark Agarose LE
A1701	Benchmark Scientific	1 PC	EUR 120.11

Benchmark Agarose LE
A1705	Benchmark Scientific	1 PC	EUR 180.4

Benchmark Agarose HR
A1801-HR	Benchmark Scientific	1 PC	EUR 165.08

Benchmark Agarose LM
A1801-LM	Benchmark Scientific	1 PC	EUR 311.86

Agarose Ultra Pure
BA01101	Bioatlas	100g	EUR 147.6
Description: Ultrapure agarose for separation and further analysis of PCR products.

Agarose Ultra Pure
BA01102	Bioatlas	500g	EUR 399.6
Description: Ultrapure agarose for separation and further analysis of PCR products.

ClearSight Agarose Tablets
BA50202	Bioatlas	100pcs	EUR 165.6
Description: Ultrapure agarose for separation and further analysis of PCR products.

Agarose, Molecular Grade
BIO-41025	Bioline	500g	Ask for price

Agarose, Molecular Grade
BIO-41026	Bioline	100g	Ask for price

V5 agarose beads
AE029-100ul	Abclonal	100 ul	Ask for price

V5 agarose beads
AE029-200ul	Abclonal	200 ul	EUR 273.6

V5 agarose beads
AE029-20ul	Abclonal	20 ul	Ask for price

V5 agarose beads
AE029-50ul	Abclonal	50 ul	Ask for price

Agarose, low EEO
GE0001-100G	Glentham Life Sciences	100 g	EUR 160.8

Agarose, low EEO
GE0001-1KG	Glentham Life Sciences	1 kg	EUR 724.8

Agarose, low EEO
GE0001-250G	Glentham Life Sciences	250 g	EUR 265.2

Agarose, low EEO
GE0001-25G	Glentham Life Sciences	25 g	EUR 84

Agarose, low EEO
GE0001-500G	Glentham Life Sciences	500 g	EUR 418.8

Agarose, low EEO
GE0001-5G	Glentham Life Sciences	5 g	EUR 58.8

Agarose, medium EEO
GE4750-100G	Glentham Life Sciences	100 g	EUR 199.2

Agarose, medium EEO
GE4750-500G	Glentham Life Sciences	500 g	EUR 514.8

LC3A Agarose, CF
PR15104CF	Neuromics	500 ul	EUR 403.2

RanBP1 Agarose Beads
STA-421	Cell Biolabs	400 µg	EUR 686.4
Description: RanBP1 Agarose Beads selectively pull down the active form of Ran. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.

Agarose IEF 10g
17046801	Scientific Laboratory Supplies	EACH	EUR 224.58

KT3 antibody (Agarose Immobilized)
60R-KG002AS	Fitzgerald	200 ul	EUR 548.4
Description: Goat polyclonal KT3 antibody (Agarose Immobilized)

Nickel NTA Agarose Resin
6BCL-NTANi-100	ABTBeads	100 ml	EUR 781.2

Nickel NTA Agarose Resin
6BCL-NTANi-25	ABTBeads	25 ml	EUR 260.4

Nickel NTA Agarose Resin
6BCL-NTANi-500	ABTBeads	500 ml	EUR 3100.8

Protein A-Agarose Resin
55000	AAT Bioquest	1 mL	EUR 158.4

Protein A-Agarose Resin
55005	AAT Bioquest	5 mL	EUR 367.2

HiQTM Low Melting Agarose
A0223-002	GenDepot	25g	EUR 343.2

HiQTM Low Melting Agarose
A0223-010	GenDepot	100g	EUR 699.6

Agarose 3:1, 100g
A0226-010	GenDepot	100g	EUR 578.4

Sepro Agarose LE Tablet
A0250-025	GenDepot	25 Tabs (12.5g)	EUR 123.6

Sepro Agarose LE Tablet
A0250-050	GenDepot	500 Tabs (250g)	EUR 465.6

AGAROSE 25G-125G -500G
A01-118-10kg	Alphabiosciences	10 kg	Ask for price

AGAROSE 25G-125G -500G
A01-118-2kg	Alphabiosciences	2kg	Ask for price

AGAROSE 25G-125G -500G
A01-118-500g	Alphabiosciences	500 g	EUR 592.8

Protein L Agarose Resin
4BCLPL-10	ABTBeads	10 ml	EUR 471.6

Protein L Agarose Resin
4BCLPL-2	ABTBeads	2 ml	EUR 236.4

Protein L Agarose Resin
4BCLPL-5	ABTBeads	5 ml	EUR 340.8

Agarose for DNA electrophoresis
16-0027-500	Medicago	500 g	EUR 410.4

Rabbit IgG-Agarose conjugate
20009-AS-1	Alpha Diagnostics	0.5 ml	EUR 196.8

Agarose B, Low EEO
AB0014	Bio Basic	50g	EUR 99.67

Agarose, Low melting gel
AB0015	Bio Basic	10g	EUR 96.54

nAb Agarose Control 0.5ml
ABP-NAB-ACNTRL5	Allele Biotech	500 µl	Ask for price

GFP-nAb Agarose 0.25ml
ABP-NAB-GFPA025	Allele Biotech	250 µl	Ask for price

GFP-nAb Agarose 0.5ml
ABP-NAB-GFPA050	Allele Biotech	500 µl	Ask for price

GFP-nAb Agarose 1ml
ABP-NAB-GFPA100	Allele Biotech	1 ml	Ask for price

mNeonGreen-nAb Agarose 0.25ml
ABP-NAB-MNGA025	Allele Biotech	250 µl	Ask for price

mNeonGreen-nAb Agarose 0.5ml
ABP-NAB-MNGA050	Allele Biotech	500 µl	Ask for price

mNeonGreen-nAb Agarose 1.0ml
ABP-NAB-MNGA100	Allele Biotech	1.0 ml	Ask for price

mTFP-nAb Agarose 0.25ml
ABP-NAB-MTFPA025	Allele Biotech	25 µg	Ask for price

mTFP-nAb Agarose 0.5ml
ABP-NAB-MTFPA050	Allele Biotech	500 µl	Ask for price

mTFP-nAb Agarose 1ml
ABP-NAB-MTFPA100	Allele Biotech	1 ml	Ask for price

RFP-nAb Agarose 0.25ml
ABP-NAB-RFPA025	Allele Biotech	250 µl	Ask for price

RFP-nAb Agarose 0.5ml
ABP-NAB-RFPA050	Allele Biotech	500 µl	Ask for price

RFP-nAb Agarose 1ml
ABP-NAB-RFPA100	Allele Biotech	1 ml	Ask for price

Benchmark Agarose 3:1
A1801-31	Benchmark Scientific	1 PC	EUR 343.18

Protein A Agarose Resin
BSP095-7	Bio Basic	1ml	EUR 112.2

Protein A Agarose Resin
BSP095-8	Bio Basic	5ml	EUR 294.9

Glutathione-Agarose (Aff matrix)
GSSH15-AS	Alpha Diagnostics	1 ml	EUR 343.2

Protein A Agarose Resin
PA09-100	ABTBeads	100 ml	EUR 2512.8

Protein A Agarose Resin
PA09-25	ABTBeads	25 ml	EUR 1110

Protein A Agarose Resin
PA09-5	ABTBeads	5 ml	EUR 349.2

Protein G Agarose Resin
SF022-PG	Bio Basic	5ml	EUR 930

PAK1 PBD Agarose Beads
STA-411	Cell Biolabs	400 ?g	EUR 686.4
Description: PAK1 PBD Agarose Beads selectively pull down the active form of Rac and Cdc42. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.

Rhotekin RBD Agarose Beads
STA-412	Cell Biolabs	400 ?g	EUR 686.4
Description: Rhotekin RBD Agarose Beads selectively pull down the active form of Rho. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.

RalGDS RBD Agarose Beads
STA-418	Cell Biolabs	400 µg	EUR 686.4
Description: RalGDS RBD Agarose Beads selectively pull down the active form of Rap. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.

GGA3 PBD Agarose Beads
STA-419	Cell Biolabs	400 µg	EUR 686.4
Description: GGA3 PBD Agarose Beads selectively pull down the active form of Arf. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.

RalBP1 PBD Agarose Beads
STA-420	Cell Biolabs	400 µg	EUR 686.4
Description: RalBP1 RBD Agarose Beads selectively pull down the active form of Ral. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg.

Streptavidin 6HC Agarose Resin
STV6HC-10	ABTBeads	10 ml	EUR 478.8

Streptavidin 6HC Agarose Resin
STV6HC-20	ABTBeads	20 ml	EUR 822

Streptavidin 6HC Agarose Resin
STV6HC-5	ABTBeads	5 ml	EUR 284.4

Agarose LE Low EEO
AXY1000	Scientific Laboratory Supplies	PK10	EUR 2058.84

Agarose LE Low EEO
AXY1002	Scientific Laboratory Supplies	PK10	EUR 8391.54

Agarose LM Low Melting
AXY1004	Scientific Laboratory Supplies	PK10	EUR 10277.1

Agarose LM Low Melting
AXY1006	Scientific Laboratory Supplies	PK10	EUR 5669.22

Agarose MS PCR Screening
AXY1008	Scientific Laboratory Supplies	PK10	EUR 5567.76

Agarose LM 50 g
DD777025	Scientific Laboratory Supplies	EACH	EUR 296.4

Hi-Bind? Protein G-Agarose
6513-1	Biovision		EUR 280.8

Hi-Bind? Protein G-Agarose
6513-100	Biovision		EUR 5468.4

Hi-Bind? Protein G-Agarose
6513-1000	Biovision		Ask for price

Hi-Bind? Protein G-Agarose
6513-25	Biovision		EUR 2114.4

Hi-Bind? Protein G-Agarose
6513-5	Biovision		EUR 679.2

Hi-Bind? Protein A-Agarose
6520-1	Biovision		EUR 196.8

Hi-Bind? Protein A-Agarose
6520-100	Biovision		EUR 4105.2

Hi-Bind? Protein A-Agarose
6520-1000	Biovision		Ask for price

Hi-Bind? Protein A-Agarose
6520-25	Biovision		EUR 1886.4

Hi-Bind? Protein A-Agarose
6520-5	Biovision		EUR 594

Hi-Bind? Protein A-Agarose
6520-500	Biovision		EUR 11739.6

Agarose Standard 50bp - 50.000 bp
604-001	GeneOn	100g	EUR 91.2

Agarose Standard 50bp - 50.000 bp
604-005	GeneOn	1x500g	EUR 222

Agarose Standard 50bp - 50.000 bp
604-005-10	GeneOn	10x500g	EUR 1594.8

Agarose Standard 50bp - 50.000 bp
604-005-2	GeneOn	2x500g	EUR 406.8

Agarose Standard 50bp - 50.000 bp
604-005-5	GeneOn	5x500g	EUR 886.8

AE-6111 Agarose EP Kit
2322178	Atto	1unit	EUR 1035.6
Description: 1 el ect r ophor esi s chamber i ncl udi ng. safet y l i dwi t h power l eads, a 12 x 16 cm gel t r ay wi t h 2 dams, 1 comb hol der and 1 comb for 1 mm, 26 &

Agarose GPG/LMP low melt
A2501-100000	ApexBio	100 g	EUR 510
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays.

Agarose GPG/LMP low melt
A2501-25000	ApexBio	25 g	EUR 184.8
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays.

Protein A Agarose IP Reagent
abx097210-2ml	Abbexa	2 ml	EUR 326.4

Protein G Agarose IP Reagent
abx097211-2ml	Abbexa	2 ml	EUR 326.4

GFP-nAb Magnetic Agarose 0.25ml
ABP-NAB-GFPX025	Allele Biotech	250 µl	Ask for price

GFP-nAb Magnetic Agarose 0.5ml
ABP-NAB-GFPX050	Allele Biotech	500 µl	Ask for price

GFP-nAb Magnetic Agarose 1ml
ABP-NAB-GFPX100	Allele Biotech	1 ml	Ask for price

mTFP-nAb Magnetic Agarose 0.25ml
ABP-NAB-MTFPX025	Allele Biotech	250 µg	Ask for price

mTFP-nAb Magnetic Agarose 0.5ml
ABP-NAB-MTFPX050	Allele Biotech	500 µl	Ask for price

mTFP-nAb Magnetic Agarose 1ml
ABP-NAB-MTFPX100	Allele Biotech	1 ml	Ask for price

RFP-nAb Magnetic Agarose 0.25ml
ABP-NAB-RFPX025	Allele Biotech	250 µl	Ask for price

RFP-nAb Magnetic Agarose 0.5ml
ABP-NAB-RFPX050	Allele Biotech	500 µl	Ask for price

Results: Using the molecular network analysis method (MN), a total of 145 compounds identified, where 13 was identified using reference compounds. Seventy-seven chemical markers are also detected between raw and processed FZ. The results of identification of the markers of the chemicals are also verified by the analysis of orthogonal partial partial discriminant (OPLS-DA). Quantitative results indicate that the contents of 12 important components are all decreased, especially the diagester of alkaloid (ddas), after processing.