Natural products down plants against pseudomonas aeruginosa and staphylococcus aureus: antibiophilm activities and molecular mechanisms

Natural products down plants against pseudomonas aeruginosa and staphylococcus aureus: antibiophilm activities and molecular mechanisms

Bacteria are social organisms that are able to build complex structures, such as biofilms, namely a very organized surface microorganism community, wrapped in its own extracellular matrix. Biofilms are usually related to many health problems because their formation increases resistance to antibiotics and antimicrobial agents, such as in the case of pseudomonas aeruginosa and staphylococcus aureus, two human pathogens that cause the main concern. P. Aeruginosa is responsible for severe nosocomial infections, the most often includes ventilator related pneumonia, while S. aureus causes several problems, such as skin infections, septic arthritis, and endocarditis, for only a few.

Literary data shows that natural products from plants, bacteria, fungi, and marine organisms have been shown to be effective as an anti-biofilm agent, inhibiting the formation of polymer matrices, suppressing cell adhesion and attachment, and reducing the production of virulence factors, thus blocking the quorum sensing network , Here, we focus on plant derivative chemicals, and provide literature reviews that are updated on the nature of anti-biofilms, flavonoids, alkaloids, and phenolic compounds. In addition, every time information is available, we also report the mechanism of action.

Bioactive Chrysin and Luteolin flavonoid protection measures on the formation of glyoxal of the final product of advanced gliking and human serum albumin aggregation: in vitro and molecular docking analysis

Modification of post-translational proteins by nonenzymatic (neg) and age accumulation are two underlying factors associated with long-term pathogenesis in diabetes. Glyoxal (GO) is an intermediary reactive that has the ability to modify proteins and produce age at a faster level. Human Serum Albumin (HSA) becomes the most abundant serum protein has a higher opportunity to be modified by the neg. The main objective of this study is to investigate the potential of Chrysin and Luteolin as an antigry and antifibrillation agent in mediated glication and the formation of HSA mediated. The formation of age is confirmed from spectral measurement of absorption and fluorescence.

Both flavonoids are able to satisfy the intensity of fluorescence in age in vitro indicating antiglica properties of molecules. Fibril formation in the modified HSA is confirmed by Thioflavin T (ENT) and flavonoids fluorescence tests found to provide antifibrillation properties in vitro. The results of the docking suggest that both flavonoids interact with various amino acid residues of iia subdomain, including licens are vulnerable to glication and arginine through non-covalent strength and further stabilize the HSA structure, which further explains their action mechanism as an antiglicate agent and antifibrillation.

Comprehensive quality evaluation of Fuzi and its processed products through UPLC-QTOF / MS integration combined MS / MS based spectral molecular networks with multivariate statistical analysis and HPLC-MS / MS.

Aconiti Lateralis Radix Praeparata (Chinese name is Fuzi, FZ), lateral root or son of asonitum Carmichaelii debx. (Ranunculaceae), is a controversial traditional Chinese medicine (TCM) distributed universally and applied in many countries, such as China, Japan, Korea and India. FZ can be used to treat various diseases, including rheumatic fever, rheumatism, painful joints, syncope, collapse, bronchial asthma, some endocrinal disorders, etc. However, the quality control and assessment of FZ challenges due to clear and high toxicological risks, and only processed products are permitted clinically used in accordance with relative safety regulations. As a result, it is necessary to analyze all chemical compositions and dynamic FZ changes before and after processing. Overcoming chemical changes from raw and processed products is a way to reduce toxicity.

Natural products down plants against pseudomonas aeruginosa and staphylococcus aureus: antibiophilm activities and molecular mechanisms
The purpose of this study: In this article, the entire Chemical Composition of FZ was analyzed, the difference between FZ RAW and processed was evaluated, and the possibility of factors that influenced the decrease in processed FZ toxicity described from the perspective of its chemical composition using qualitative and quantitative analysis methods.

Materials and Methods: New strategies of many data collection and processing based on ultra-performance liquid chromatography coupled with the Mass Spectrometry method for quadrupole time (UPLC-QTOF / MS) in positive ion mode, along with the natural product of the global social molecular network (GNP) and analysis Multivariate Statistics, was established to systematically identify the chemical constituents of FZ and investigate comprehensively chemical markers that can be used to distinguish FZ processed with vinegar and honey from raw products. Combined with the results of qualitative analysis, 12 components, including 8 chemical markers compounds and 4 toxicity components, are analyzed quantitatively by using high-performance liquid chromatography equipped with triple-quadrupole mass spectrometry (HPLC-MS).

Nickel NTA Agarose Resin

6BCL-NTANi-25 25 ml
EUR 242.1

Nickel NTA Agarose Resin

6BCL-NTANi-500 500 ml
EUR 3483.72

Nickel Agarose Extrachel™

6RREXCH-100 100 ml
EUR 1361.82

Nickel Agarose Extrachel™

6RREXCH-25 25 ml
EUR 371.85

Nickel Agarose Extrachel™

6RREXCH-500 500 ml
EUR 5206.85

4% Magnetic T1 Agarose Bead

4MGT1-100 100 ml
EUR 803.25

4% Magnetic T1 Agarose Bead

4MGT1-25 25 ml
EUR 226.28

4% Magnetic Large Agarose Bead

4MGT3-100 100 ml
EUR 803.25

4% Magnetic Large Agarose Bead

4MGT3-25 25 ml
EUR 226.28

Protein A Agarose Resin 4 Rapid Run™

4RRPA-25 25 ml
EUR 1514.98

Protein A Agarose Resin 4 Rapid Run™

4RRPA-5 5 ml
EUR 409.12

Protein A Agarose Resin 4 Rapid Run™

4RRPA-500 500 ml
EUR 4270

Protein G Agarose Resin 4 Rapid Run™

4RRPG-100 100 ml
EUR 1699.5

Protein G Agarose Resin 4 Rapid Run™

4RRPG-25 25 ml
EUR 1002.55

Protein G Agarose Resin 4 Rapid Run™

4RRPG-5 5 ml
EUR 334.18

Protein G Agarose Resin 4 Rapid Run™

4RRPG-500 500 ml
EUR 4875

Protein A/G Agarose Resin 4 Rapid Run™

4RRPAG-05 0,5 ml
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Protein A/G Agarose Resin 4 Rapid Run™

4RRPAG-1 1 ml
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Protein A/G Agarose Resin 4 Rapid Run™

4RRPAG-2 2 ml
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ExtraBind Protein G Agarose Resin 6 Rapid Run™

6RRPG2-100 100 ml
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ExtraBind Protein G Agarose Resin 6 Rapid Run™

6RRPG2-25 25 ml
EUR 1060

ExtraBind Protein G Agarose Resin 6 Rapid Run™

6RRPG2-5 5 ml
EUR 345

ExtraBind Protein G Agarose Resin 6 Rapid Run™

6RRPG2-500 500 ml
EUR 4920

Agarose T5, High Resolution

A2131-100G 100G
EUR 352
Description: Biotechnology

Agarose T5, High Resolution

A2131-10G 10G
EUR 61.6
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Agarose T5, High Resolution

A2131-25G 25G
EUR 110
Description: Biotechnology

Nickel NTA Agarose Cartridges 5ml, 1 or 5 cartridges with 5 ml of resin each one

6BCL-NTANiCtg5-1 1 x 5ml
EUR 157.15

Nickel NTA Agarose Cartridges 5ml, 1 or 5 cartridges with 5 ml of resin each one

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Agarose

20-abx098147
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Agarose

abx082006-100g 100 g
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Agarose

abx082007-100g 100 g
EUR 309.6

Agarose

abx082008-100g 100 g
EUR 276

Agarose

A12140 5G
EUR 113.97

Agarose

20-abx186369
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AGAROSE

IB70035 25GM
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AGAROSE

IB70040 100GM
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AGAROSE

IB70041 250GM
EUR 271

AGAROSE

IB70042 500GM
EUR 476.49

AGAROSE

IB70045 1KG
EUR 816.44

AGAROSE

IB70046 2.5KG
EUR 2162.07

AGAROSE

IB70047 5KG
EUR 3780.9

Agarose

G060-1 100 g
EUR 185
Description: Agarose, derived from agar, is a polymeric cross-linked polysaccharide that is mainly used in gel electrophoresis. The lattice bonds formed in the gel allow proteins of different sizes to travel at different speeds down the gel.

Agarose

G060-2 500 g
EUR 415
Description: Agarose, derived from agar, is a polymeric cross-linked polysaccharide that is mainly used in gel electrophoresis. The lattice bonds formed in the gel allow proteins of different sizes to travel at different speeds down the gel.

Agarose

Z5050007 500 g
EUR 575

Agarose

Z5050013 100 g
EUR 141

Agarose

MB080-100G 1 unit
EUR 533.09
Description: Agarose

Agarose

MB080-10G 1 unit
EUR 59.42
Description: Agarose

Agarose

MB094-100G 1 unit
EUR 55.96
Description: Agarose

Agarose

MB094-1KG 1 unit
EUR 894.45
Description: Agarose

Agarose

MB094-500G 1 unit
EUR 223.32
Description: Agarose

Agarose

abx098147-100l 100 µl
EUR 262.5

Agarose

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Agarose

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Agarose

abx299902-100g 100 µg
EUR 350

Agarose

abx299902-20g 20 µg
EUR 262.5

Agarose

abx299902-50g 50 µg
EUR 287.5

Agarose

abx461004-1096tests 10 × 96 tests Ask for price

Agarose

abx461004-596tests 5 × 96 tests
EUR 850

Agarose

abx461004-96tests 96 tests
EUR 425

Agarose (3%) & LE Agarose 1% in 1X TAE Buffer

40120844-1 100 mL
EUR 33.86

Agarose (3%) & LE Agarose 1% in 1X TAE Buffer

40120844-2 250 mL
EUR 53.99

Agarose (3%) & LE Agarose 1% in 1X TAE Buffer

40120844-3 500 mL
EUR 81.44

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01132-34 100G
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Agarose -LE

01132-92 25G
EUR 44.8

Agarose -ME

01133-24 100G
EUR 122.5

Agarose -ME

01133-82 25G
EUR 44.1

Agarose -HE

01134-14 100G
EUR 122.5

Agarose I

40100104-1 25 g
EUR 31.11

Agarose I

40100104-2 100 g
EUR 78.67

Agarose I

40100104-3 250 g
EUR 171.95

Agarose I

40100104-4 500 g
EUR 288.65

Agarose I

40100104-5 1 kg
EUR 483.16

Agarose A

D0012 50g
EUR 96.54

Agarose LE

A-1270-100 100 g
EUR 139.2

Agarose LE

A-1270-500 500 g
EUR 349.2

Agarose HR

A-1280-100 100 g
EUR 219.6

Agarose HR

A-1280-500 500 g
EUR 822

Agarose GA

A-1290-100 100 g
EUR 123.6

Agarose GA

A-1290-500 500 g
EUR 306

Agarose LM

A-1300-100 100 g
EUR 230.4

Agarose LM

A-1300-25 25 g
EUR 123.6

Agarose M

AB0013 50g
EUR 91.32

AGAROSE, P.F.G.E.

IB70060 25GM
EUR 41.75

AGAROSE, P.F.G.E.

IB70061 50GM
EUR 58.73

AGAROSE, P.F.G.E.

IB70062 100GM
EUR 104.39

AGAROSE, P.F.G.E.

IB70063 250GM
EUR 252.05

AGAROSE, P.F.G.E.

IB70064 500GM
EUR 498.97

AGAROSE, P.F.G.E.

IB70065 1KG
EUR 956.47

LE Agarose

PCR-269L 2 x 500g
EUR 808.2

LE Agarose

PCR-269S 2 x 100g
EUR 202

LM Agarose

PCR-277L 5 x 50g
EUR 890

LM Agarose

PCR-277S 50g
EUR 222.5

Agarose L.M.P

abx082386-100l 100 µl
EUR 175

Agarose L.M.P

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Agarose L.M.P

abx082386-200l 200 µl
EUR 375

Agarose -LGT

01136-52 25G
EUR 115.5

Agarose -LGT

01136-94 100G
EUR 329

Agarose -HGT

01137-84 100G
EUR 122.5

Agarose -HGS

01143-52 25G
EUR 49

Agarose -HGS

01143-94 100G
EUR 133

Agarose, Sepro

A0224-010 100g
EUR 262.8

Agarose, Sepro

A0224-020 200g
EUR 343.2

Agarose, Sepro

A0224-050 500g
EUR 522

Agarose (blank)

AC-001L 5ml
EUR 266

Agarose (blank)

AC-001S 1ml
EUR 68.9

Biotin Agarose

20-544 5ml/Unit
EUR 242.32
Description: Cross-linked Beads, 4%

Biotin Agarose

20-545 10ml/Unit
EUR 456.66
Description: Cross-linked Beads, 4%

Agarose Tablets

BA50201 100pcs
EUR 130.8
Description: Ultrapure agarose for separation and further analysis of PCR products.

Agarose Tablets

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Agarose Tablets

abx299714-20g 20 µg
EUR 462.5

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Agarose 3:1, 100g

A0226-010 100g
EUR 578.4

AGAROSE, ULTRASIEVE

IB70054 25GM
EUR 146.27

AGAROSE, ULTRASIEVE

IB70055 250GM
EUR 858.4

Agarose GPG/LE

A2500-100000 100 g
EUR 170.4
Description: Agar is a galactan polysaccharides which extracted from the intracellular matrix in marine algae Rhodophyta. Agarose is a neutral polymer component of agar. Agarose solution transforms into gel after heating. Gel is formed resulting from double helical formation from molecular chains.

Agarose GPG/LE

A2500-500000 500 g
EUR 560.4
Description: Agar is a galactan polysaccharides which extracted from the intracellular matrix in marine algae Rhodophyta. Agarose is a neutral polymer component of agar. Agarose solution transforms into gel after heating. Gel is formed resulting from double helical formation from molecular chains.

Agarose GPG/ME

A2502-100000 100 g
EUR 226.8
Description: analytical and preparative electrophoresis of DNA >1000bpnorthern/southern blottingserum protein electrophoresis.

Agarose GPG/ME

A2502-500000 500 g
EUR 811.2
Description: analytical and preparative electrophoresis of DNA >1000bpnorthern/southern blottingserum protein electrophoresis.

Agarose GP-36

01139-22 25G
EUR 48.3

Agarose GP-36

01139-64 100G
EUR 132.3

Agarose GP-42

01140-24 100G
EUR 129.5

Agarose, Low EEO

40100152-1 50 g
EUR 75.92

Agarose, Low EEO

40100152-2 100 g
EUR 140.08

Agarose, Low EEO

40100152-3 500 g
EUR 446.01

Agarose, low EEO

GE0001-1 1
EUR 569.5

Agarose, low EEO

GE0001-100 100
EUR 102.7

Agarose, low EEO

GE0001-100G 100 g
EUR 160.8

Agarose, low EEO

GE0001-1KG 1 kg
EUR 724.8

Agarose, low EEO

GE0001-25 25
EUR 39.4

Agarose, low EEO

GE0001-250 250
EUR 189.8

Agarose, low EEO

GE0001-250G 250 g
EUR 265.2

Agarose, low EEO

GE0001-25G 25 g
EUR 84

Agarose, low EEO

GE0001-5 5
EUR 19

Agarose, low EEO

GE0001-500 500
EUR 316.4

Agarose, low EEO

GE0001-500G 500 g
EUR 418.8

Agarose, low EEO

GE0001-5G 5 g
EUR 58.8

LC3A Agarose, CF

PR15104CF 500 ul
EUR 403.2

Agarose Low EEO

abx299901-100g 100 µg
EUR 425

Agarose Low EEO

abx299901-20g 20 µg
EUR 300

Agarose Low EEO

abx299901-50g 50 µg
EUR 350

RFP-Tag mouse monoclonal antibody, clone 3G5, Agarose, Agarose

AM26655AG-N 200 µl Ask for price

Agarose, High EEO

40100148-1 25 g
EUR 51.77

Agarose, High EEO

40100148-2 100 g
EUR 143.93

V5 agarose beads

AE029 200μL
EUR 87.75

V5 agarose beads

AE029-100ul 100 ul Ask for price

V5 agarose beads

AE029-200ul 200 ul
EUR 273.6

V5 agarose beads

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Agarose, high EEO

GE7747-1 1
EUR 660.6

Agarose, high EEO

GE7747-250 250
EUR 227.8

Agarose, high EEO

RM187-100G 1 unit
EUR 174.08
Description: Agarose, high EEO

Agarose, high EEO

RM187-10G 1 unit
EUR 18.22
Description: Agarose, high EEO

Agarose, high EEO

RM187-500G 1 unit
EUR 748.81
Description: Agarose, high EEO

Agarose High EEO

abx299903-100g 100 µg
EUR 387.5

Agarose High EEO

abx299903-20g 20 µg
EUR 275

Agarose High EEO

abx299903-50g 50 µg
EUR 325

IBI BASIC AGAROSE

IB70070 100GM
EUR 85.59

IBI BASIC AGAROSE

IB70071 250GM
EUR 167.87

IBI BASIC AGAROSE

IB70072 500GM
EUR 405.82

IBI BASIC AGAROSE

IB70073 1KG
EUR 795.26

IBI BASIC AGAROSE

IB70074 5KG
EUR 3457.29

Benchmark Agarose LE, 100g

A1701 1 PC
EUR 139.87

Benchmark Agarose LE, 500g

A1705 1 PC
EUR 487.4

Protein L Agarose

20-533 2ml/Unit
EUR 273.72
Description: Cross-linked Beads, 4%

Protein L Agarose

20-534 5ml/Unit
EUR 542.41
Description: Cross-linked Beads, 4%

Results: Using the molecular network analysis method (MN), a total of 145 compounds identified, where 13 was identified using reference compounds. Seventy-seven chemical markers are also detected between raw and processed FZ. The results of identification of the markers of the chemicals are also verified by the analysis of orthogonal partial partial discriminant (OPLS-DA). Quantitative results indicate that the contents of 12 important components are all decreased, especially the diagester of alkaloid (ddas), after processing.

 

 

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