Bacteria are social organisms that are able to build complex structures, such as biofilms, namely a very organized surface microorganism community, wrapped in its own extracellular matrix. Biofilms are usually related to many health problems because their formation increases resistance to antibiotics and antimicrobial agents, such as in the case of pseudomonas aeruginosa and staphylococcus aureus, two human pathogens that cause the main concern. P. Aeruginosa is responsible for severe nosocomial infections, the most often includes ventilator related pneumonia, while S. aureus causes several problems, such as skin infections, septic arthritis, and endocarditis, for only a few.
Literary data shows that natural products from plants, bacteria, fungi, and marine organisms have been shown to be effective as an anti-biofilm agent, inhibiting the formation of polymer matrices, suppressing cell adhesion and attachment, and reducing the production of virulence factors, thus blocking the quorum sensing network , Here, we focus on plant derivative chemicals, and provide literature reviews that are updated on the nature of anti-biofilms, flavonoids, alkaloids, and phenolic compounds. In addition, every time information is available, we also report the mechanism of action.
Bioactive Chrysin and Luteolin flavonoid protection measures on the formation of glyoxal of the final product of advanced gliking and human serum albumin aggregation: in vitro and molecular docking analysis
Modification of post-translational proteins by nonenzymatic (neg) and age accumulation are two underlying factors associated with long-term pathogenesis in diabetes. Glyoxal (GO) is an intermediary reactive that has the ability to modify proteins and produce age at a faster level. Human Serum Albumin (HSA) becomes the most abundant serum protein has a higher opportunity to be modified by the neg. The main objective of this study is to investigate the potential of Chrysin and Luteolin as an antigry and antifibrillation agent in mediated glication and the formation of HSA mediated. The formation of age is confirmed from spectral measurement of absorption and fluorescence.
Both flavonoids are able to satisfy the intensity of fluorescence in age in vitro indicating antiglica properties of molecules. Fibril formation in the modified HSA is confirmed by Thioflavin T (ENT) and flavonoids fluorescence tests found to provide antifibrillation properties in vitro. The results of the docking suggest that both flavonoids interact with various amino acid residues of iia subdomain, including licens are vulnerable to glication and arginine through non-covalent strength and further stabilize the HSA structure, which further explains their action mechanism as an antiglicate agent and antifibrillation.
Comprehensive quality evaluation of Fuzi and its processed products through UPLC-QTOF / MS integration combined MS / MS based spectral molecular networks with multivariate statistical analysis and HPLC-MS / MS.
Aconiti Lateralis Radix Praeparata (Chinese name is Fuzi, FZ), lateral root or son of asonitum Carmichaelii debx. (Ranunculaceae), is a controversial traditional Chinese medicine (TCM) distributed universally and applied in many countries, such as China, Japan, Korea and India. FZ can be used to treat various diseases, including rheumatic fever, rheumatism, painful joints, syncope, collapse, bronchial asthma, some endocrinal disorders, etc. However, the quality control and assessment of FZ challenges due to clear and high toxicological risks, and only processed products are permitted clinically used in accordance with relative safety regulations. As a result, it is necessary to analyze all chemical compositions and dynamic FZ changes before and after processing. Overcoming chemical changes from raw and processed products is a way to reduce toxicity.
The purpose of this study: In this article, the entire Chemical Composition of FZ was analyzed, the difference between FZ RAW and processed was evaluated, and the possibility of factors that influenced the decrease in processed FZ toxicity described from the perspective of its chemical composition using qualitative and quantitative analysis methods.
Materials and Methods: New strategies of many data collection and processing based on ultra-performance liquid chromatography coupled with the Mass Spectrometry method for quadrupole time (UPLC-QTOF / MS) in positive ion mode, along with the natural product of the global social molecular network (GNP) and analysis Multivariate Statistics, was established to systematically identify the chemical constituents of FZ and investigate comprehensively chemical markers that can be used to distinguish FZ processed with vinegar and honey from raw products. Combined with the results of qualitative analysis, 12 components, including 8 chemical markers compounds and 4 toxicity components, are analyzed quantitatively by using high-performance liquid chromatography equipped with triple-quadrupole mass spectrometry (HPLC-MS).
 Agarose L.M.P |
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20-abx082386 |
Abbexa |
|
|
|
|
 Agarose LE |
|
A-1270-100 |
ABTBeads |
100 g |
EUR 139.2 |
Agarose LE |
|
A-1270-500 |
ABTBeads |
500 g |
EUR 349.2 |
 Agarose HR |
|
A-1280-100 |
ABTBeads |
100 g |
EUR 219.6 |
Agarose HR |
|
A-1280-500 |
ABTBeads |
500 g |
EUR 822 |
 Agarose GA |
|
A-1290-100 |
ABTBeads |
100 g |
EUR 123.6 |
Agarose GA |
|
A-1290-500 |
ABTBeads |
500 g |
EUR 306 |
 Agarose LM |
|
A-1300-100 |
ABTBeads |
100 g |
EUR 230.4 |
Agarose LM |
|
A-1300-25 |
ABTBeads |
25 g |
EUR 123.6 |
 Agarose, Sepro |
|
A0224-010 |
GenDepot |
100g |
EUR 262.8 |
Agarose, Sepro |
|
A0224-020 |
GenDepot |
200g |
EUR 343.2 |
Agarose, Sepro |
|
A0224-050 |
GenDepot |
500g |
EUR 522 |
 Agarose Tablets |
|
BA50201 |
Bioatlas |
100pcs |
EUR 130.8 |
|
Description: Ultrapure agarose for separation and further analysis of PCR products. |
 Agarose 3:1, 100g |
|
A0226-010 |
GenDepot |
100g |
EUR 578.4 |
 Agarose GPG/LE |
|
A2500-100000 |
ApexBio |
100 g |
EUR 170.4 |
|
Description: Agar is a galactan polysaccharides which extracted from the intracellular matrix in marine algae Rhodophyta. Agarose is a neutral polymer component of agar. Agarose solution transforms into gel after heating. Gel is formed resulting from double helical formation from molecular chains. |
Agarose GPG/LE |
|
A2500-500000 |
ApexBio |
500 g |
EUR 560.4 |
|
Description: Agar is a galactan polysaccharides which extracted from the intracellular matrix in marine algae Rhodophyta. Agarose is a neutral polymer component of agar. Agarose solution transforms into gel after heating. Gel is formed resulting from double helical formation from molecular chains. |
 Agarose GPG/ME |
|
A2502-100000 |
ApexBio |
100 g |
EUR 226.8 |
|
Description: analytical and preparative electrophoresis of DNA >1000bpnorthern/southern blottingserum protein electrophoresis. |
Agarose GPG/ME |
|
A2502-500000 |
ApexBio |
500 g |
EUR 811.2 |
|
Description: analytical and preparative electrophoresis of DNA >1000bpnorthern/southern blottingserum protein electrophoresis. |
 LC3A Agarose, CF |
|
PR15104CF |
Neuromics |
500 ul |
EUR 403.2 |
 V5 agarose beads |
|
AE029-100ul |
Abclonal |
100 ul |
Ask for price |
V5 agarose beads |
|
AE029-200ul |
Abclonal |
200 ul |
EUR 273.6 |
V5 agarose beads |
|
AE029-20ul |
Abclonal |
20 ul |
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V5 agarose beads |
|
AE029-50ul |
Abclonal |
50 ul |
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 GelRed Agarose LE |
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41029-50G |
Biotium |
50G |
EUR 384 |
|
Description: Minimum order quantity: 1 unit of 50G |
GelRed Agarose LE |
|
41029-5G |
Biotium |
5G |
EUR 124.8 |
|
Description: Minimum order quantity: 1 unit of 5G |
 Agarose Ultra Pure |
|
BA01101 |
Bioatlas |
100g |
EUR 147.6 |
|
Description: Ultrapure agarose for separation and further analysis of PCR products. |
Agarose Ultra Pure |
|
BA01102 |
Bioatlas |
500g |
EUR 399.6 |
|
Description: Ultrapure agarose for separation and further analysis of PCR products. |
 GelGreen Agarose LE |
|
41030-50G |
Biotium |
50G |
EUR 384 |
|
Description: Minimum order quantity: 1 unit of 50G |
GelGreen Agarose LE |
|
41030-5G |
Biotium |
5G |
EUR 124.8 |
|
Description: Minimum order quantity: 1 unit of 5G |
 Agarose, Resolute GPG |
|
A2503-100000 |
ApexBio |
100 g |
EUR 477.6 |
|
Description: resolve differences in bp length by as little as 2%finely resolve DNA fragments from 20-1000bp, rivals polyacrylamidegels are extremely clear and easy to handle due to high gel strengthno detectable inhibition of DNA ligase or the following restriction enzymes:BamH I, Hind II, EcoR I.. |
 Biotin Agarose Resin |
|
4BCL-BI-10 |
ABTBeads |
10 ml |
EUR 493.2 |
Biotin Agarose Resin |
|
4BCL-BI-5 |
ABTBeads |
5 ml |
EUR 282 |
 RanBP1 Agarose Beads |
|
STA-421 |
Cell Biolabs |
400 µg |
EUR 686.4 |
|
Description: RanBP1 Agarose Beads selectively pull down the active form of Ran. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 Agarose B, Low EEO |
|
AB0014 |
Bio Basic |
50g |
EUR 99.67 |
|
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 HiQTM Standard Agarose |
|
A0222-020 |
GenDepot |
200g |
EUR 300 |
HiQTM Standard Agarose |
|
A0222-050 |
GenDepot |
500g |
EUR 537.6 |
 Agarose, Molecular Grade |
|
BIO-41025 |
Bioline |
500g |
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 Agarose, Molecular Grade |
|
BIO-41026 |
Bioline |
100g |
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 HyAgarose? 3:1 Agarose, 100g |
|
R901231-100g |
ACTGene |
each |
EUR 398.4 |
 HyAgarose? 3:1 Agarose, 500g |
|
R901231-500g |
ACTGene |
each |
EUR 1369.2 |
 Nickel Agarose Extrachel |
|
6RREXCH-100 |
ABTBeads |
100 ml |
EUR 1263.6 |
Nickel Agarose Extrachel |
|
6RREXCH-25 |
ABTBeads |
25 ml |
EUR 380.4 |
Nickel Agarose Extrachel |
|
6RREXCH-500 |
ABTBeads |
500 ml |
EUR 4698 |
 GFP-nAb Agarose 0.5ml |
|
ABP-NAB-GFPA050 |
Allele Biotech |
500 µl |
Ask for price |
|
|
 RFP-nAb Agarose 0.5ml |
|
ABP-NAB-RFPA050 |
Allele Biotech |
500 µl |
Ask for price |
|
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 Glutathione Agarose Resin |
|
4B-GLU-10 |
ABTBeads |
10 ml |
EUR 207.6 |
Glutathione Agarose Resin |
|
4B-GLU-100 |
ABTBeads |
100 ml |
EUR 1195.2 |
 GFP-nAb Agarose 0.25ml |
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ABP-NAB-GFPA025 |
Allele Biotech |
250 µl |
Ask for price |
|
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 mTFP-nAb Agarose 0.5ml |
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ABP-NAB-MTFPA050 |
Allele Biotech |
500 µl |
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 mTFP-nAb Agarose 1ml |
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ABP-NAB-MTFPA100 |
Allele Biotech |
1 ml |
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 RFP-nAb Agarose 0.25ml |
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ABP-NAB-RFPA025 |
Allele Biotech |
250 µl |
Ask for price |
|
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 PAK1 PBD Agarose Beads |
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STA-411 |
Cell Biolabs |
400 ?g |
EUR 686.4 |
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Description: PAK1 PBD Agarose Beads selectively pull down the active form of Rac and Cdc42. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 GGA3 PBD Agarose Beads |
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STA-419 |
Cell Biolabs |
400 µg |
EUR 686.4 |
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Description: GGA3 PBD Agarose Beads selectively pull down the active form of Arf. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 Agarose StandardRoomTemperature |
|
FYA002-100G |
Yeastern Biotech |
100 g |
Ask for price |
Agarose StandardRoomTemperature |
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FYA002-500G |
Yeastern Biotech |
500 g |
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 ClearSight Agarose Tablets |
|
BA50202 |
Bioatlas |
100pcs |
EUR 165.6 |
|
Description: Ultrapure agarose for separation and further analysis of PCR products. |
 Sepro Agarose LE Tablet |
|
A0250-025 |
GenDepot |
25 Tabs (12.5g) |
EUR 123.6 |
Sepro Agarose LE Tablet |
|
A0250-050 |
GenDepot |
500 Tabs (250g) |
EUR 465.6 |
 Agarose, Low melting gel |
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AB0015 |
Bio Basic |
10g |
EUR 96.54 |
|
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 nAb Agarose Control 0.5ml |
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ABP-NAB-ACNTRL5 |
Allele Biotech |
500 µl |
Ask for price |
|
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 mTFP-nAb Agarose 0.25ml |
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ABP-NAB-MTFPA025 |
Allele Biotech |
25 µg |
Ask for price |
|
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 Protein A Agarose Resin |
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BSP095-7 |
Bio Basic |
1ml |
EUR 112.2 |
|
|
Protein A Agarose Resin |
|
BSP095-8 |
Bio Basic |
5ml |
EUR 294.9 |
|
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 Nickel NTA Agarose Resin |
|
6BCL-NTANi-100 |
ABTBeads |
100 ml |
EUR 781.2 |
Nickel NTA Agarose Resin |
|
6BCL-NTANi-25 |
ABTBeads |
25 ml |
EUR 260.4 |
Nickel NTA Agarose Resin |
|
6BCL-NTANi-500 |
ABTBeads |
500 ml |
EUR 3100.8 |
 Protein L Agarose Resin |
|
4BCLPL-10 |
ABTBeads |
10 ml |
EUR 471.6 |
Protein L Agarose Resin |
|
4BCLPL-2 |
ABTBeads |
2 ml |
EUR 236.4 |
Protein L Agarose Resin |
|
4BCLPL-5 |
ABTBeads |
5 ml |
EUR 340.8 |
Protein A Agarose Resin |
|
PA09-100 |
ABTBeads |
100 ml |
EUR 2512.8 |
Protein A Agarose Resin |
|
PA09-25 |
ABTBeads |
25 ml |
EUR 1110 |
Protein A Agarose Resin |
|
PA09-5 |
ABTBeads |
5 ml |
EUR 349.2 |
 Protein G Agarose Resin |
|
SF022-PG |
Bio Basic |
5ml |
EUR 930 |
|
|
 RalGDS RBD Agarose Beads |
|
STA-418 |
Cell Biolabs |
400 µg |
EUR 686.4 |
|
Description: RalGDS RBD Agarose Beads selectively pull down the active form of Rap. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 RalBP1 PBD Agarose Beads |
|
STA-420 |
Cell Biolabs |
400 µg |
EUR 686.4 |
|
Description: RalBP1 RBD Agarose Beads selectively pull down the active form of Ral. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 HiQTM Low Melting Agarose |
|
A0223-002 |
GenDepot |
25g |
EUR 343.2 |
HiQTM Low Melting Agarose |
|
A0223-010 |
GenDepot |
100g |
EUR 699.6 |
 Rhotekin RBD Agarose Beads |
|
STA-412 |
Cell Biolabs |
400 ?g |
EUR 686.4 |
|
Description: Rhotekin RBD Agarose Beads selectively pull down the active form of Rho. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 AE-6111 Agarose EP Kit |
|
2322178 |
Atto |
1unit |
EUR 1035.6 |
|
Description: 1 el ect r ophor esi s chamber i ncl udi ng. safet y l i dwi t h power l eads, a 12 x 16 cm gel t r ay wi t h 2 dams, 1 comb hol der and 1 comb for 1 mm, 26 & |
 Agarose GPG/LMP low melt |
|
A2501-100000 |
ApexBio |
100 g |
EUR 510 |
|
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays. |
Agarose GPG/LMP low melt |
|
A2501-25000 |
ApexBio |
25 g |
EUR 184.8 |
|
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays. |
 CM Rapid Run Agarose Bead |
|
CMRR-25 |
ABTBeads |
25 ml |
EUR 122.4 |
CM Rapid Run Agarose Bead |
|
CMRR-300 |
ABTBeads |
300 ml |
EUR 590.4 |
 SP Rapid Run Agarose Bead |
|
SPRR-25 |
ABTBeads |
25 ml |
EUR 122.4 |
SP Rapid Run Agarose Bead |
|
SPRR-300 |
ABTBeads |
300 ml |
EUR 590.4 |
 Raf-1 RBD Agarose Beads |
|
STA-410 |
Cell Biolabs |
400 ?g |
EUR 686.4 |
|
Description: Raf-1 RBD Agarose Beads selectively pull down the active form of Ras. Beads are colored to allow for a visual check. These are the same beads supplied with our Ras Activation Assays. 400 µg. |
 mNeonGreen-nAb Agarose 0.5ml |
|
ABP-NAB-MNGA050 |
Allele Biotech |
500 µl |
Ask for price |
|
|
 mNeonGreen-nAb Agarose 1.0ml |
|
ABP-NAB-MNGA100 |
Allele Biotech |
1.0 ml |
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|
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 mNeonGreen-nAb Agarose 0.25ml |
|
ABP-NAB-MNGA025 |
Allele Biotech |
250 µl |
Ask for price |
|
|
 Agarose Standard 50bp - 50.000 bp |
|
604-001 |
GeneOn |
100g |
EUR 91.2 |
Agarose Standard 50bp - 50.000 bp |
|
604-005 |
GeneOn |
1x500g |
EUR 222 |
Agarose Standard 50bp - 50.000 bp |
|
604-005-10 |
GeneOn |
10x500g |
EUR 1594.8 |
Agarose Standard 50bp - 50.000 bp |
|
604-005-2 |
GeneOn |
2x500g |
EUR 406.8 |
Agarose Standard 50bp - 50.000 bp |
|
604-005-5 |
GeneOn |
5x500g |
EUR 886.8 |
 DEAE Rapid Run Agarose Bead |
|
DEAERR-25 |
ABTBeads |
25 ml |
EUR 122.4 |
DEAE Rapid Run Agarose Bead |
|
DEAERR-300 |
ABTBeads |
300 ml |
EUR 590.4 |
 HyAgarose? 3:1 Agarose, 10g |
|
R901231-10g |
ACTGene |
each |
EUR 99.6 |
 HyAgarose? 3:1 Agarose, 50g |
|
R901231-50g |
ACTGene |
each |
EUR 242.4 |
 Streptavidin 6HC Agarose Resin |
|
STV6HC-10 |
ABTBeads |
10 ml |
EUR 478.8 |
Streptavidin 6HC Agarose Resin |
|
STV6HC-20 |
ABTBeads |
20 ml |
EUR 822 |
Streptavidin 6HC Agarose Resin |
|
STV6HC-5 |
ABTBeads |
5 ml |
EUR 284.4 |
 Agarose for DNA electrophoresis |
|
16-0027-500 |
Medicago |
500 g |
EUR 410.4 |
 Protein A Agarose IP Reagent |
|
abx097210-2ml |
Abbexa |
2 ml |
EUR 326.4 |
|
|
 Protein G Agarose IP Reagent |
|
abx097211-2ml |
Abbexa |
2 ml |
EUR 326.4 |
|
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) KT3 antibody (Agarose Immobilized) |
|
60R-KG002AS |
Fitzgerald |
200 ul |
EUR 548.4 |
|
Description: Goat polyclonal KT3 antibody (Agarose Immobilized) |
 GFP-nAb Magnetic Agarose 0.5ml |
|
ABP-NAB-GFPX050 |
Allele Biotech |
500 µl |
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|
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 GFP-nAb Magnetic Agarose 1ml |
|
ABP-NAB-GFPX100 |
Allele Biotech |
1 ml |
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|
|
 RFP-nAb Magnetic Agarose 0.5ml |
|
ABP-NAB-RFPX050 |
Allele Biotech |
500 µl |
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|
|
 RFP-nAb Magnetic Agarose 1ml |
|
ABP-NAB-RFPX100 |
Allele Biotech |
1 ml |
Ask for price |
|
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 Hi-Bind? Ni QR Agarose Beads |
|
6562-1 |
Biovision |
each |
EUR 130.8 |
Hi-Bind? Ni QR Agarose Beads |
|
6562-10 |
Biovision |
each |
EUR 183.6 |
Hi-Bind? Ni QR Agarose Beads |
|
6562-100 |
Biovision |
each |
EUR 848.4 |
Hi-Bind? Ni QR Agarose Beads |
|
6562-500 |
Biovision |
each |
EUR 3184.8 |
 GFP-nAb Magnetic Agarose 0.25ml |
|
ABP-NAB-GFPX025 |
Allele Biotech |
250 µl |
Ask for price |
|
|
 mTFP-nAb Magnetic Agarose 0.5ml |
|
ABP-NAB-MTFPX050 |
Allele Biotech |
500 µl |
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|
|
 mTFP-nAb Magnetic Agarose 1ml |
|
ABP-NAB-MTFPX100 |
Allele Biotech |
1 ml |
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|
|
 RFP-nAb Magnetic Agarose 0.25ml |
|
ABP-NAB-RFPX025 |
Allele Biotech |
250 µl |
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|
|
 Agarose Tablets , DNase/RNase Free |
|
BIO-41027 |
Bioline |
300g |
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 mTFP-nAb Magnetic Agarose 0.25ml |
|
ABP-NAB-MTFPX025 |
Allele Biotech |
250 µg |
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|
|
 nAb Magnetic Agarose Control 0.5ml |
|
ABP-NAB-XCNTRL5 |
Allele Biotech |
500 µl |
Ask for price |
|
|
 AE-6100 Agarose EP Kit, 1 mm |
|
2322153 |
Atto |
1unit |
EUR 832.8 |
|
Description: 1 el ect r ophor esi s chamber i ncl udi ng safet y l i dwi t h power l eads, 1 gel t r ay, 1 r ol l of seal t ape, 1 comb hol der , 1 1-mm 10-wel l comb, and 1 cl i p. |
 Agarose II, Low Gelling Temperature |
|
CH002 |
ABM |
25 g |
EUR 274.8 |
Agarose II, Low Gelling Temperature |
|
CH003 |
ABM |
100 g |
EUR 525.6 |
 Protein A&G Agarose IP Reagent |
|
abx097212-2ml |
Abbexa |
2 ml |
EUR 326.4 |
|
|
 HyAgarose? HR Agarose, PCR Grade, 100g |
|
R9012HR-100g |
ACTGene |
each |
EUR 398.4 |
Results: Using the molecular network analysis method (MN), a total of 145 compounds identified, where 13 was identified using reference compounds. Seventy-seven chemical markers are also detected between raw and processed FZ. The results of identification of the markers of the chemicals are also verified by the analysis of orthogonal partial partial discriminant (OPLS-DA). Quantitative results indicate that the contents of 12 important components are all decreased, especially the diagester of alkaloid (ddas), after processing.
