Bacteria are social organisms that are able to build complex structures, such as biofilms, namely a very organized surface microorganism community, wrapped in its own extracellular matrix. Biofilms are usually related to many health problems because their formation increases resistance to antibiotics and antimicrobial agents, such as in the case of pseudomonas aeruginosa and staphylococcus aureus, two human pathogens that cause the main concern. P. Aeruginosa is responsible for severe nosocomial infections, the most often includes ventilator related pneumonia, while S. aureus causes several problems, such as skin infections, septic arthritis, and endocarditis, for only a few.
Literary data shows that natural products from plants, bacteria, fungi, and marine organisms have been shown to be effective as an anti-biofilm agent, inhibiting the formation of polymer matrices, suppressing cell adhesion and attachment, and reducing the production of virulence factors, thus blocking the quorum sensing network , Here, we focus on plant derivative chemicals, and provide literature reviews that are updated on the nature of anti-biofilms, flavonoids, alkaloids, and phenolic compounds. In addition, every time information is available, we also report the mechanism of action.
Bioactive Chrysin and Luteolin flavonoid protection measures on the formation of glyoxal of the final product of advanced gliking and human serum albumin aggregation: in vitro and molecular docking analysis
Modification of post-translational proteins by nonenzymatic (neg) and age accumulation are two underlying factors associated with long-term pathogenesis in diabetes. Glyoxal (GO) is an intermediary reactive that has the ability to modify proteins and produce age at a faster level. Human Serum Albumin (HSA) becomes the most abundant serum protein has a higher opportunity to be modified by the neg. The main objective of this study is to investigate the potential of Chrysin and Luteolin as an antigry and antifibrillation agent in mediated glication and the formation of HSA mediated. The formation of age is confirmed from spectral measurement of absorption and fluorescence.
Both flavonoids are able to satisfy the intensity of fluorescence in age in vitro indicating antiglica properties of molecules. Fibril formation in the modified HSA is confirmed by Thioflavin T (ENT) and flavonoids fluorescence tests found to provide antifibrillation properties in vitro. The results of the docking suggest that both flavonoids interact with various amino acid residues of iia subdomain, including licens are vulnerable to glication and arginine through non-covalent strength and further stabilize the HSA structure, which further explains their action mechanism as an antiglicate agent and antifibrillation.
Comprehensive quality evaluation of Fuzi and its processed products through UPLC-QTOF / MS integration combined MS / MS based spectral molecular networks with multivariate statistical analysis and HPLC-MS / MS.
Aconiti Lateralis Radix Praeparata (Chinese name is Fuzi, FZ), lateral root or son of asonitum Carmichaelii debx. (Ranunculaceae), is a controversial traditional Chinese medicine (TCM) distributed universally and applied in many countries, such as China, Japan, Korea and India. FZ can be used to treat various diseases, including rheumatic fever, rheumatism, painful joints, syncope, collapse, bronchial asthma, some endocrinal disorders, etc. However, the quality control and assessment of FZ challenges due to clear and high toxicological risks, and only processed products are permitted clinically used in accordance with relative safety regulations. As a result, it is necessary to analyze all chemical compositions and dynamic FZ changes before and after processing. Overcoming chemical changes from raw and processed products is a way to reduce toxicity.
The purpose of this study: In this article, the entire Chemical Composition of FZ was analyzed, the difference between FZ RAW and processed was evaluated, and the possibility of factors that influenced the decrease in processed FZ toxicity described from the perspective of its chemical composition using qualitative and quantitative analysis methods.
Materials and Methods: New strategies of many data collection and processing based on ultra-performance liquid chromatography coupled with the Mass Spectrometry method for quadrupole time (UPLC-QTOF / MS) in positive ion mode, along with the natural product of the global social molecular network (GNP) and analysis Multivariate Statistics, was established to systematically identify the chemical constituents of FZ and investigate comprehensively chemical markers that can be used to distinguish FZ processed with vinegar and honey from raw products. Combined with the results of qualitative analysis, 12 components, including 8 chemical markers compounds and 4 toxicity components, are analyzed quantitatively by using high-performance liquid chromatography equipped with triple-quadrupole mass spectrometry (HPLC-MS).
 Agarose |
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abx082008-100g |
Abbexa |
100 g |
EUR 230 |
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Agarose |
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G060-1 |
ABM |
100 g |
EUR 176 |
Agarose |
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G060-2 |
ABM |
500 g |
EUR 369 |
Agarose |
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Z5050007 |
Biochain |
500 g |
EUR 615 |
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Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time. |
Agarose |
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Z5050013 |
Biochain |
100 g |
EUR 189 |
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Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time. |
 Fish Thyroxine,T5 ELISA Kit |
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CN-00301F2 |
ChemNorm |
48T |
EUR 304 |
 Rat thyroxine,T5 ELISA Kit |
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CN-01989R2 |
ChemNorm |
48T |
EUR 307 |
 ?-1,4-Gal-T5 Polyclonal Antibody |
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ES3750-100ul |
ELK Biotech |
100ul |
EUR 279 |
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Description: A Rabbit Polyclonal antibody against ?-1,4-Gal-T5 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
?-1,4-Gal-T5 Polyclonal Antibody |
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ES3750-50ul |
ELK Biotech |
50ul |
EUR 207 |
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Description: A Rabbit Polyclonal antibody against ?-1,4-Gal-T5 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
 Anti- beta-1,4-Gal-T5 Antibody |
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A09370 |
BosterBio |
100ul |
EUR 397 |
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Description: Rabbit Polyclonal Antibody for beta-1,4-Gal-T5 Antibody (B4GALT5) detection. Tested with WB in Human, Mouse. |
 GalNAc-T5, soluble fragment, Human Recombinant |
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P1217-5 |
Biovision |
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EUR 240 |
) anti-R-PTP Mu (T5-AF1A8) |
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LF-MA0354 |
Abfrontier |
100 ul |
EUR 334 |
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Description: Mouse monoclonal to R-PTP Mu |
 Anti-beta-1,4-Gal-T5 antibody |
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STJ96375 |
St John's Laboratory |
200 µl |
EUR 197 |
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Description: Rabbit polyclonal to beta-1,4-Gal-T5. |
 Agarose, Sepro |
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A0224-010 |
GenDepot |
100g |
EUR 219 |
Agarose, Sepro |
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A0224-020 |
GenDepot |
200g |
EUR 286 |
Agarose, Sepro |
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A0224-050 |
GenDepot |
500g |
EUR 435 |
 Agarose LE |
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A-1270-100 |
ABTBeads |
100 g |
EUR 116 |
Agarose LE |
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A-1270-500 |
ABTBeads |
500 g |
EUR 291 |
 Agarose HR |
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A-1280-100 |
ABTBeads |
100 g |
EUR 183 |
Agarose HR |
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A-1280-500 |
ABTBeads |
500 g |
EUR 685 |
 Agarose GA |
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A-1290-100 |
ABTBeads |
100 g |
EUR 103 |
Agarose GA |
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A-1290-500 |
ABTBeads |
500 g |
EUR 255 |
 Agarose LM |
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A-1300-100 |
ABTBeads |
100 g |
EUR 192 |
Agarose LM |
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A-1300-25 |
ABTBeads |
25 g |
EUR 103 |
 Agarose L.M.P |
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20-abx082386 |
Abbexa |
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 Agarose Tablets |
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BA50201 |
Bioatlas |
100pcs |
EUR 109 |
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Description: Ultrapure agarose for separation and further analysis of PCR products. |
 Agarose StandardRoomTemperature |
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FYA002-100G |
Yeastern Biotech |
100 g |
Ask for price |
Agarose StandardRoomTemperature |
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FYA002-500G |
Yeastern Biotech |
500 g |
Ask for price |
 Rat Neonatal Thyroxine,NN-T5 ELISA Kit |
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CN-01992R2 |
ChemNorm |
48T |
EUR 338 |
 Beta-1, 4-Gal-T5 Polyclonal Antibody |
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ABP52751-003ml |
Abbkine |
0.03ml |
EUR 158 |
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Description: A polyclonal antibody for detection of Beta-1, 4-Gal-T5 from Human, Mouse. This Beta-1, 4-Gal-T5 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human ?-1,4-Gal-T5 at AA range: 290-370 |
Beta-1, 4-Gal-T5 Polyclonal Antibody |
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ABP52751-01ml |
Abbkine |
0.1ml |
EUR 289 |
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Description: A polyclonal antibody for detection of Beta-1, 4-Gal-T5 from Human, Mouse. This Beta-1, 4-Gal-T5 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human ?-1,4-Gal-T5 at AA range: 290-370 |
Beta-1, 4-Gal-T5 Polyclonal Antibody |
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ABP52751-02ml |
Abbkine |
0.2ml |
EUR 414 |
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Description: A polyclonal antibody for detection of Beta-1, 4-Gal-T5 from Human, Mouse. This Beta-1, 4-Gal-T5 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human ?-1,4-Gal-T5 at AA range: 290-370 |
 Nickel Agarose Extrachel |
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6RREXCH-100 |
ABTBeads |
100 ml |
EUR 1053 |
Nickel Agarose Extrachel |
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6RREXCH-25 |
ABTBeads |
25 ml |
EUR 317 |
Nickel Agarose Extrachel |
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6RREXCH-500 |
ABTBeads |
500 ml |
EUR 3915 |
 Protein A-Agarose |
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6526-100 |
Biovision |
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EUR 2959 |
Protein A-Agarose |
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6526-1000 |
Biovision |
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Ask for price |
 Glutathione Agarose Resin |
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4B-GLU-10 |
ABTBeads |
10 ml |
EUR 173 |
Glutathione Agarose Resin |
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4B-GLU-100 |
ABTBeads |
100 ml |
EUR 996 |
 Biotin Agarose Resin |
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4BCL-BI-10 |
ABTBeads |
10 ml |
EUR 411 |
Biotin Agarose Resin |
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4BCL-BI-5 |
ABTBeads |
5 ml |
EUR 235 |
 GelRed Agarose LE |
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41029-50G |
Biotium |
50G |
EUR 320 |
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Description: Minimum order quantity: 1 unit of 50G |
GelRed Agarose LE |
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41029-5G |
Biotium |
5G |
EUR 104 |
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Description: Minimum order quantity: 1 unit of 5G |
 GelGreen Agarose LE |
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41030-50G |
Biotium |
50G |
EUR 320 |
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Description: Minimum order quantity: 1 unit of 50G |
GelGreen Agarose LE |
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41030-5G |
Biotium |
5G |
EUR 104 |
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Description: Minimum order quantity: 1 unit of 5G |
 HiQTM Standard Agarose |
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A0222-020 |
GenDepot |
200g |
EUR 250 |
HiQTM Standard Agarose |
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A0222-050 |
GenDepot |
500g |
EUR 448 |
 V5 agarose beads |
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AE029-100ul |
Abclonal |
100 ul |
Ask for price |
V5 agarose beads |
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AE029-200ul |
Abclonal |
200 ul |
EUR 228 |
V5 agarose beads |
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AE029-20ul |
Abclonal |
20 ul |
Ask for price |
V5 agarose beads |
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AE029-50ul |
Abclonal |
50 ul |
Ask for price |
 Agarose Ultra Pure |
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BA01101 |
Bioatlas |
100g |
EUR 123 |
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Description: Ultrapure agarose for separation and further analysis of PCR products. |
Agarose Ultra Pure |
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BA01102 |
Bioatlas |
500g |
EUR 333 |
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Description: Ultrapure agarose for separation and further analysis of PCR products. |
 ClearSight Agarose Tablets |
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BA50202 |
Bioatlas |
100pcs |
EUR 138 |
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Description: Ultrapure agarose for separation and further analysis of PCR products. |
 Agarose, Molecular Grade |
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BIO-41025 |
Bioline |
500g |
Ask for price |
 Agarose, Molecular Grade |
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BIO-41026 |
Bioline |
100g |
Ask for price |
 Benchmark Agarose HR |
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A1801-HR |
BenchMark |
1 PC |
EUR 137.57 |
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 Benchmark Agarose LM |
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A1801-LM |
BenchMark |
1 PC |
EUR 259.88 |
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 Agarose GPG/LE |
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A2500-100000 |
ApexBio |
100 g |
EUR 142 |
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Description: Agar is a galactan polysaccharides which extracted from the intracellular matrix in marine algae Rhodophyta. Agarose is a neutral polymer component of agar. Agarose solution transforms into gel after heating. Gel is formed resulting from double helical formation from molecular chains. |
Agarose GPG/LE |
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A2500-500000 |
ApexBio |
500 g |
EUR 467 |
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Description: Agar is a galactan polysaccharides which extracted from the intracellular matrix in marine algae Rhodophyta. Agarose is a neutral polymer component of agar. Agarose solution transforms into gel after heating. Gel is formed resulting from double helical formation from molecular chains. |
 Agarose GPG/ME |
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A2502-100000 |
ApexBio |
100 g |
EUR 189 |
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Description: analytical and preparative electrophoresis of DNA >1000bpnorthern/southern blottingserum protein electrophoresis. |
Agarose GPG/ME |
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A2502-500000 |
ApexBio |
500 g |
EUR 676 |
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Description: analytical and preparative electrophoresis of DNA >1000bpnorthern/southern blottingserum protein electrophoresis. |
 Agarose, Resolute GPG |
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A2503-100000 |
ApexBio |
100 g |
EUR 398 |
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Description: resolve differences in bp length by as little as 2%finely resolve DNA fragments from 20-1000bp, rivals polyacrylamidegels are extremely clear and easy to handle due to high gel strengthno detectable inhibition of DNA ligase or the following restriction enzymes:BamH I, Hind II, EcoR I.. |
 Benchmark Agarose LE |
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A1700 |
BenchMark |
1 PC |
EUR 321.65 |
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Benchmark Agarose LE |
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A1701 |
BenchMark |
1 PC |
EUR 100.09 |
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Benchmark Agarose LE |
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A1705 |
BenchMark |
1 PC |
EUR 150.33 |
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 LC3A Agarose, CF |
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PR15104CF |
Neuromics |
500 ul |
EUR 336 |
 RanBP1 Agarose Beads |
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STA-421 |
Cell Biolabs |
400 µg |
EUR 572 |
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Description: RanBP1 Agarose Beads selectively pull down the active form of Ran. Beads are colored to allow for a visual check. These are the same beads supplied with our Small GTPase Activation Assays. 400 µg. |
 Nickel NTA Agarose Resin |
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6BCL-NTANi-100 |
ABTBeads |
100 ml |
EUR 651 |
Nickel NTA Agarose Resin |
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6BCL-NTANi-25 |
ABTBeads |
25 ml |
EUR 217 |
Nickel NTA Agarose Resin |
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6BCL-NTANi-500 |
ABTBeads |
500 ml |
EUR 2584 |
 Agarose for DNA electrophoresis |
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16-0027-500 |
Medicago |
500 g |
EUR 342 |
) KT3 antibody (Agarose Immobilized) |
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60R-KG002AS |
Fitzgerald |
200 ul |
EUR 457 |
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Description: Goat polyclonal KT3 antibody (Agarose Immobilized) |
 Protein L Agarose Resin |
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4BCLPL-10 |
ABTBeads |
10 ml |
EUR 393 |
Protein L Agarose Resin |
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4BCLPL-2 |
ABTBeads |
2 ml |
EUR 197 |
Protein L Agarose Resin |
|
4BCLPL-5 |
ABTBeads |
5 ml |
EUR 284 |
Results: Using the molecular network analysis method (MN), a total of 145 compounds identified, where 13 was identified using reference compounds. Seventy-seven chemical markers are also detected between raw and processed FZ. The results of identification of the markers of the chemicals are also verified by the analysis of orthogonal partial partial discriminant (OPLS-DA). Quantitative results indicate that the contents of 12 important components are all decreased, especially the diagester of alkaloid (ddas), after processing.
