Epigentek

Epigentek Chromoplasts Chromatin Extraction Kit

Background Info

Chromatin immunoprecipitation (ChIP) gives an advantageous system for finding out protein-DNA interplay. With ChIP, the experimenter can decide if a particular protein binds to the precise sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) methods. As an illustration, the measurement of the quantity of methylated histone H3 at lysine 9 (meH3-K9) related to a particular gene promoter area beneath fairly a couple of circumstances may presumably be achieved by means of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale may presumably be detected by ChIP-chip. Notably, the ChIP methodology with particular antibodies straight in path of fairly a couple of transcriptional elements is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Bundle deal is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in an easy and fast format. Chromatin ready by this bundle could also be utilized in varied chromatin immunoprecipitation strategies. Furthermore it is the truly useful methodology for purchasing chromatin required by Epigentek’s one-hour ChIP methodology utilizing the ChromaFlash One-Step ChIP Bundle deal. The remoted chromatin can also be utilized in a number of chromatin-related options equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly quick course of: your complete course of from cell/tissue pattern to ready-to-use chromatin is lower than 60 minutes.
  • Useful and versatile: the bundle is appropriate for preparing each native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for fairly a couple of evaluation workflows that require every intact or fragmented chromatin, together with ChIP, in vitro protein-DNA interplay evaluation, nuclear enzyme assay, and so forth.
Epigentek
Epigentek

 

Precept & Course of

The ChromaFlash Chromatin Extraction Bundle deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the pattern, with or with out cross-linking, are damaged down utilizing the geared up lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the related temperature.

Beginning Gives & Enter Quantity
Beginning gives can embrace fairly a couple of tissue or cell samples equal to cells from flask or microplate cultured cells, fashionable and frozen tissues, and so forth. The quantity of cells and tissues for every preparation may presumably be 1 x 105 to five x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter quantity must be 1 to five x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is roughly four ug per 106  cells or per 50 mg tissues.

Background Info
Chromatin immunoprecipitation (ChIP) gives an advantageous system for finding out protein-DNA interplay. With ChIP, the experimenter can decide if a particular protein binds to the precise sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) methods. As an illustration, the measurement of the quantity of methylated histone H3 at lysine 9 (meH3-K9) related to a particular gene promoter area beneath fairly a couple of circumstances may presumably be achieved by means of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale may presumably be detected by ChIP-chip. Notably, a ChIP methodology with particular antibodies straight in path of fairly a couple of transcriptional elements is broadly in demand.

Precept & Course of
The ChromaFlash Plant Chromatin Extraction Bundle deal accommodates your complete reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the pattern, with or with out cross-linking, are damaged down utilizing the geared up lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the related temperature.
ExoDNA? Extraction Kit
K1242-25
EUR 588
ExoDNA? Extraction Kit
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EUR 881
miRNA Extraction Kit
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EUR 551
Quick Gel Extraction Kit
20-abx098085
  • EUR 286.00
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  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.
Up RNA Extraction Kit
20-abx098087
  • EUR 258.00
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  • 100 ml
  • 3 ml
  • Shipped within 5-10 working days.
Plant RNA extraction Kit
20-abx098088
  • EUR 258.00
  • EUR 105.00
  • 100 ml
  • 3 ml
  • Shipped within 5-10 working days.
Membrane Protein Extraction Kit
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Description: Membrane Protein Extraction Kit for use in the research laboratory
Mammalian Cell Extraction Kit
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EUR 381
Description: Mammalian Cell Extraction Kit for use in the research laboratory
Total RNA Extraction Kit
K2014005 1 kit
EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein Extraction Kit
K2113-50 50 assays
EUR 544
Mammalian Cell Extraction Kit
K2114-500 500 assays
EUR 321
Membrane Protein Extraction Kit
K268-50
EUR 539
Mammalian Cell Extraction Kit
K269-500
EUR 294
Total Protein Extraction Kit
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EUR 370
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein Extraction Kit
K3014005 1 kit
EUR 382
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Plant Tissue Extraction Kit
K296-50
EUR 457
EpiQuik Nuclear Extraction Kit
OP-0002 100 Assays
EUR 292.15
Description: kits suitable for this type of research
Genomic DNA Extraction Kit
K5016005 1 kit
EUR 329
Exosomal RNA Extraction Kit
P200R,-part-of-P200 NULL
EUR 0
Exosomal DNA Extraction Kit
P230-25 NULL
EUR 0
Exosomal DNA Extraction Kit
P230-50 NULL
EUR 0
Bacterial RNA Extraction Kit
R403-01 100 rxn
EUR 155
Total Protein Extraction Kit
BSP003 50Preps
EUR 109.16
  • Product category: Molecular Biology Kits/Protein - Extraction/Animal
Plant Protein Extraction Kit
BSP004 20Preps
EUR 110.9
  • Product category: Molecular Biology Kits/Protein - Extraction/Plant
Nuclear Protein Extraction Kit
BSP009 50Preps
EUR 89.15
  • Product category: Molecular Biology Kits/Protein - Extraction/Animal
FFPE Protein Extraction Kit
BSP058 50Preps
EUR 102.2
  • Product category: Molecular Biology Kits/Protein - Extraction/FFPE Tissue
Frit Kit
FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

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