Epigentek

Epigentek Chromoplasts Chromatin Extraction Kit

Background Info

Chromatin immunoprecipitation (ChIP) gives an advantageous system for finding out protein-DNA interplay. With ChIP, the experimenter can decide if a particular protein binds to the precise sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) methods. As an illustration, the measurement of the quantity of methylated histone H3 at lysine 9 (meH3-K9) related to a particular gene promoter area beneath fairly a couple of circumstances may presumably be achieved by means of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale may presumably be detected by ChIP-chip. Notably, the ChIP methodology with particular antibodies straight in path of fairly a couple of transcriptional elements is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Bundle deal is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in an easy and fast format. Chromatin ready by this bundle could also be utilized in varied chromatin immunoprecipitation strategies. Furthermore it is the truly useful methodology for purchasing chromatin required by Epigentek’s one-hour ChIP methodology utilizing the ChromaFlash One-Step ChIP Bundle deal. The remoted chromatin can also be utilized in a number of chromatin-related options equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly quick course of: your complete course of from cell/tissue pattern to ready-to-use chromatin is lower than 60 minutes.
  • Useful and versatile: the bundle is appropriate for preparing each native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for fairly a couple of evaluation workflows that require every intact or fragmented chromatin, together with ChIP, in vitro protein-DNA interplay evaluation, nuclear enzyme assay, and so forth.
Epigentek
Epigentek

 

Precept & Course of

The ChromaFlash Chromatin Extraction Bundle deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the pattern, with or with out cross-linking, are damaged down utilizing the geared up lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the related temperature.

Beginning Gives & Enter Quantity
Beginning gives can embrace fairly a couple of tissue or cell samples equal to cells from flask or microplate cultured cells, fashionable and frozen tissues, and so forth. The quantity of cells and tissues for every preparation may presumably be 1 x 105 to five x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter quantity must be 1 to five x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is roughly four ug per 106  cells or per 50 mg tissues.

Background Info
Chromatin immunoprecipitation (ChIP) gives an advantageous system for finding out protein-DNA interplay. With ChIP, the experimenter can decide if a particular protein binds to the precise sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) methods. As an illustration, the measurement of the quantity of methylated histone H3 at lysine 9 (meH3-K9) related to a particular gene promoter area beneath fairly a couple of circumstances may presumably be achieved by means of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale may presumably be detected by ChIP-chip. Notably, a ChIP methodology with particular antibodies straight in path of fairly a couple of transcriptional elements is broadly in demand.

Precept & Course of
The ChromaFlash Plant Chromatin Extraction Bundle deal accommodates your complete reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the pattern, with or with out cross-linking, are damaged down utilizing the geared up lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the related temperature.

miRNA Extraction Kit

K1456-50 50 Preps
EUR 661.2

ExoDNA? Extraction Kit

K1242-25 each
EUR 705.6

ExoDNA? Extraction Kit

K1242-50 each
EUR 1057.2

Up RNA Extraction Kit

20-abx098087
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit 

P-2002
  • EUR 1084.86
  • EUR 312.40
  • EUR 500.50
  • EUR 738.10
  • 96 Reactions
  • 24 Reactions
  • 48 Reactions
  • 96 Reactions

Quick Gel Extraction Kit

20-abx098085
  • EUR 343.20
  • EUR 243.60
  • 200 rxns
  • 50 rxns

Plant RNA extraction Kit

20-abx098088
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

Chromatin protein Cren7 Antibody (HRP)

20-abx109204
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gel Extraction Kit (100 preps)

9K-006-0001 100preps
EUR 283.42

Gel Extraction Kit (200 preps)

9K-006-0002 200preps, 200prep
EUR 418.09

Chromatin protein Cren7 Antibody (FITC)

20-abx107787
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

FFPE Protein Extraction Kit

BSP058 50Preps
EUR 122.64

Exosomal RNA Extraction Kit

P200R,-part-of-P200 - Ask for price

Exosomal DNA Extraction Kit

P230-25 - Ask for price

Exosomal DNA Extraction Kit

P230-50 - Ask for price

Plant Tissue Extraction Kit

K296-50 each
EUR 548.4

Chromatin Modifying Protein 2A Protein

20-abx261989
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Chromatin Modifying Protein 2B Protein

20-abx262025
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Chromatin Modifying Protein 4A Protein

20-abx263203
  • EUR 276.00
  • EUR 1779.60
  • EUR 393.60
  • 1 µg
  • 50 ug
  • 5 ug

Chromatin Modifying Protein 1A Protein

20-abx263326
  • EUR 1930.80
  • EUR 393.60
  • EUR 276.00
  • 100 ug
  • 10 ug
  • 2 µg

Chromatin Protein Cren7 (creN7) Antibody

20-abx109437
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

ATRX, Chromatin Remodeler (ATRX) Antibody

20-abx325123
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

ATRX, Chromatin Remodeler (ATRX) Antibody

abx331300-100ul 100 ul
EUR 510

Total Protein Extraction Kit

BSP003 50Preps
EUR 130.99

Plant Protein Extraction Kit

BSP004 20Preps
EUR 133.08

Total Protein Extraction Kit

K3011010 1 kit
EUR 367

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 186

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