Epigentek

Epigentek Chromoplasts Chromatin Extraction Kit

Background Info

Chromatin immunoprecipitation (ChIP) gives an advantageous system for finding out protein-DNA interplay. With ChIP, the experimenter can decide if a particular protein binds to the precise sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) methods. As an illustration, the measurement of the quantity of methylated histone H3 at lysine 9 (meH3-K9) related to a particular gene promoter area beneath fairly a couple of circumstances may presumably be achieved by means of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale may presumably be detected by ChIP-chip. Notably, the ChIP methodology with particular antibodies straight in path of fairly a couple of transcriptional elements is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Bundle deal is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in an easy and fast format. Chromatin ready by this bundle could also be utilized in varied chromatin immunoprecipitation strategies. Furthermore it is the truly useful methodology for purchasing chromatin required by Epigentek’s one-hour ChIP methodology utilizing the ChromaFlash One-Step ChIP Bundle deal. The remoted chromatin can also be utilized in a number of chromatin-related options equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly quick course of: your complete course of from cell/tissue pattern to ready-to-use chromatin is lower than 60 minutes.
  • Useful and versatile: the bundle is appropriate for preparing each native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for fairly a couple of evaluation workflows that require every intact or fragmented chromatin, together with ChIP, in vitro protein-DNA interplay evaluation, nuclear enzyme assay, and so forth.
Epigentek
Epigentek

 

Precept & Course of

The ChromaFlash Chromatin Extraction Bundle deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the pattern, with or with out cross-linking, are damaged down utilizing the geared up lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the related temperature.

Beginning Gives & Enter Quantity
Beginning gives can embrace fairly a couple of tissue or cell samples equal to cells from flask or microplate cultured cells, fashionable and frozen tissues, and so forth. The quantity of cells and tissues for every preparation may presumably be 1 x 105 to five x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter quantity must be 1 to five x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is roughly four ug per 106  cells or per 50 mg tissues.

Background Info
Chromatin immunoprecipitation (ChIP) gives an advantageous system for finding out protein-DNA interplay. With ChIP, the experimenter can decide if a particular protein binds to the precise sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) methods. As an illustration, the measurement of the quantity of methylated histone H3 at lysine 9 (meH3-K9) related to a particular gene promoter area beneath fairly a couple of circumstances may presumably be achieved by means of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale may presumably be detected by ChIP-chip. Notably, a ChIP methodology with particular antibodies straight in path of fairly a couple of transcriptional elements is broadly in demand.

Precept & Course of
The ChromaFlash Plant Chromatin Extraction Bundle deal accommodates your complete reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the pattern, with or with out cross-linking, are damaged down utilizing the geared up lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the related temperature.

ExoDNA? Extraction Kit

K1242-25
EUR 705.6

ExoDNA? Extraction Kit

K1242-50
EUR 1057.2

miRNA Extraction Kit

K1456-50 50 Preps
EUR 661.2

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Total Protein Extraction Kit

BSP003 50Preps
EUR 130.99

Plant Protein Extraction Kit

BSP004 20Preps
EUR 133.08

Nuclear Protein Extraction Kit

BSP009 50Preps
EUR 106.98

FFPE Protein Extraction Kit

BSP058 50Preps
EUR 122.64

Membrane Protein Extraction Kit

55R-1357 50 assays
EUR 888
Description: Membrane Protein Extraction Kit for use in the research laboratory

Mammalian Cell Extraction Kit

55R-1358 500 assays
EUR 457.2
Description: Mammalian Cell Extraction Kit for use in the research laboratory

Quick Gel Extraction Kit

20-abx098085
  • EUR 343.20
  • EUR 243.60
  • 200 rxns
  • 50 rxns

Up RNA Extraction Kit

20-abx098087
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

Plant RNA extraction Kit

20-abx098088
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

Exosomal RNA Extraction Kit

P200R,-part-of-P200 - Ask for price

Exosomal DNA Extraction Kit

P230-25 - Ask for price

Exosomal DNA Extraction Kit

P230-50 - Ask for price

Plant Tissue Extraction Kit

K296-50
EUR 548.4

Total Protein Extraction Kit

K3011010 1 kit
EUR 444
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein Extraction Kit

K268-50
EUR 646.8

Mammalian Cell Extraction Kit

K269-500
EUR 352.8

Membrane Protein Extraction Kit

K2113-50 50 assays
EUR 652.8

Mammalian Cell Extraction Kit

K2114-500 500 assays
EUR 385.2

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 186

EpiQuik Nuclear Extraction Kit 

OP-0002-1 100 Extractions
EUR 211.2

EpiQuik Nuclear Extraction Kit 

OP-0002
  • EUR 350.58
  • EUR 211.20
  • 100 Assays
  • 100 Extractions

Membrane Protein Extraction Kit

MPE01-12S 0.5 ml
EUR 117.02
Description: This product includes 0.5 ml of 12 solutions for screening and micro-scale preparative extraction of membrane proteins (up to 1.5 mg) from the cell membranes.

Column Packing Kit

PACK-KIT 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

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