Chromatin immunoprecipitation (ChIP) gives an advantageous system for finding out protein-DNA interplay. With ChIP, the experimenter can decide if a particular protein binds to the precise sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) methods. As an illustration, the measurement of the quantity of methylated histone H3 at lysine 9 (meH3-K9) related to a particular gene promoter area beneath fairly a couple of circumstances may presumably be achieved by means of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale may presumably be detected by ChIP-chip. Notably, the ChIP methodology with particular antibodies straight in path of fairly a couple of transcriptional elements is broadly demanded.
Product Overview
The ChromaFlash Chromatin Extraction Bundle deal is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in an easy and fast format. Chromatin ready by this bundle could also be utilized in varied chromatin immunoprecipitation strategies. Furthermore it is the truly useful methodology for purchasing chromatin required by Epigentek’s one-hour ChIP methodology utilizing the ChromaFlash One-Step ChIP Bundle deal. The remoted chromatin can also be utilized in a number of chromatin-related options equal to in vitro protein-DNA binding assays and nuclear enzyme assays.
Terribly quick course of: your complete course of from cell/tissue pattern to ready-to-use chromatin is lower than 60 minutes.
Useful and versatile: the bundle is appropriate for preparing each native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
Unsheared chromatin makes it customizable for fairly a couple of evaluation workflows that require every intact or fragmented chromatin, together with ChIP, in vitro protein-DNA interplay evaluation, nuclear enzyme assay, and so forth.
Epigentek
Precept & Course of
The ChromaFlash Chromatin Extraction Bundle deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the pattern, with or with out cross-linking, are damaged down utilizing the geared up lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the related temperature.
Beginning Gives & Enter Quantity
Beginning gives can embrace fairly a couple of tissue or cell samples equal to cells from flask or microplate cultured cells, fashionable and frozen tissues, and so forth. The quantity of cells and tissues for every preparation may presumably be 1 x 105 to five x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter quantity must be 1 to five x 106 cells or 50 to 200 mg tissues. Yield of chromatin is roughly four ug per 106 cells or per 50 mg tissues.
Background Info Chromatin immunoprecipitation (ChIP) gives an advantageous system for finding out protein-DNA interplay. With ChIP, the experimenter can decide if a particular protein binds to the precise sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) methods. As an illustration, the measurement of the quantity of methylated histone H3 at lysine 9 (meH3-K9) related to a particular gene promoter area beneath fairly a couple of circumstances may presumably be achieved by means of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale may presumably be detected by ChIP-chip. Notably, a ChIP methodology with particular antibodies straight in path of fairly a couple of transcriptional elements is broadly in demand.
Precept & Course of
The ChromaFlash Plant Chromatin Extraction Bundle deal accommodates your complete reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the pattern, with or with out cross-linking, are damaged down utilizing the geared up lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the related temperature.
