Lipid Peroxidation (MDA) Assay Kit

Lipid peroxidation assay protocol abstract:

– add TBA reply to samples and requirements, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
– swap to wells of microplate
– analyze with microplate reader
For higher sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet earlier than evaluation.

Product overview

Lipid Peroxidation (MDA + HNE) Assay Bundle

Lipid peroxidation is a extensively recognized event of oxidative harm in cell membranes, lipoproteins, and completely completely different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl ldl ldl cholesterol can happen in numerous reactions. They’re usually triggered by i) free radical species equal to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated low price of hydrogen peroxide or ii) non-radical species equal to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.

Malondialdehyde (MDA) and 4-hydroxyalkenals are crucial poisonous byproducts of lipid peroxidation. So, the measurement of the parts of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a important product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Moreover, elevated ranges of HNE had been present in plasma and numerous organs beneath oxidative stress circumstances. The fact is, MDA is in loads of circumstances almost certainly basically probably the most ample particular particular person aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and a great deal of completely completely different biomolecules.

Bioquochem’s LPO assay instruments measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). As a consequence of this, this response gives a diindolylalkane (chromophore) with most absorbance contained in the area of 580-620 nm.

In our assay an indol (Reagent A) reacts rapidly with MDA and HNE in acidic medium, yielding a chromophore (C) with a excessive molar extinction coefficient at its maximal absorption wavelength of 586 nm.

• Notes

  Lipid peroxidation refers once more to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (typically in cell membranes), leading to cell harm. Quantification of lipid peroxidation is important to guage oxidative stress. Lipid peroxidation varieties reactive aldehydes equal to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are usually used as markers of lipid peroxidation, and to assay for oxidative harm / oxidative stress.

  Associated merchandise

  Analysis the oxidative stress marker and assay knowledge, or the general metabolism assay knowledge to examine additional assays for metabolites, metabolic enzymes, mitochondrial perform, and oxidative stress, and in addition to how one can assay metabolic perform in dwell cells utilizing your plate reader.

  Furthermore see the favored 4-HNE Assay Bundle ab238538 as an alternative marker of lipid peroxidation and oxidative stress.

  

  
  How completely completely different researchers have used Lipid Peroxidation Assay Bundle ab118970

  The MDA/TBARs assay instruments has been utilized in publications in quite a lot of pattern varieties, together with:
  – Human: serum¹, hippocampal principal cell extracts², A375 cultured cell lysates³, plasma and platelet samples⁴
  – Mouse: neuronal cell lysates⁵, coronary coronary coronary heart tissue extract⁶, plasma⁷, cell extracts⁸
  – Rat: hippocampal tissue extracts⁹, cardiomyocyte extracts of cultured cells¹⁰, lung lysates¹¹
  – Pig: serum¹²

  ^{References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018,  4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019}

• Platform

  Microplate reader

Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit
K2167-100	ApexBio	100 assays	EUR 703.2

Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit
22906	AAT Bioquest	200 Tests	EUR 367.2

Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit
15991	AAT Bioquest	200 tests	EUR 471.6

Myeloperoxidase (MPO) Peroxidation Activity Assay Kit
K747-100	Biovision		EUR 392.4

OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric)
STA-805	Cell Biolabs	192 assays	EUR 692.4
Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample.

Lipid A Lipopolysaccharide (LPS Lipid A / Endotoxin) Antibody
abx415762-1ml	Abbexa	1 ml	EUR 727.2

Lipid A Antibody
abx023083-1ml	Abbexa	1 ml	EUR 886.8

Lipid Extraction & Polar/Neutral Lipid Separation Combo Kit (Chloroform Free)
MET-5009-C	Cell Biolabs	50 preps	EUR 790.8
Description: This kit combines a chloroform free organic lipid extraction with further separation of polar and neutral lipids. The lipid extraction first extracts lipids from a crude lipid source to a chloroform free upper organic phase using a proprietary alcohol. After addition of a proprietary organic compound, the mixture is centrifuged to separate the phases. The recovered organic phase containing lipids is dried and then polar and neutral lipids are separated by adding a proprietary secondary alcohol and organic compound. The layers are separated, dried, and resuspended for further analysis.

Ceraplex Lipid Concentrate(1000X)
C3316-010	GenDepot	100ml	EUR 144

Ceraplex Lipid Concentrate(1000X)
C3316-050	GenDepot	500ml	EUR 295.2

Lipid Droplet Isolation Kit
MET-5011	Cell Biolabs	50 preps	EUR 498
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

Lipid Quantitation Kit (Colorimetric)
STA-613	Cell Biolabs	100 assays	EUR 637.2
Description: Cell Biolabs? Lipid Quantification Kit measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method, resulting in a simple colorimetric readout amenable to multi-well plate detection.

Lipid Quantification Kit (Fluorometric)
STA-617	Cell Biolabs	100 assays	EUR 637.2
Description: The Lipid Quantification Kit (Fluorometric) uses a fluorometric detection system to quantify neutral lipids in a convenient 96-well microplate reader. The kit includes sufficient reagents for 100 assays.

Anti-Lipid A antibody
STJ16100142	St John's Laboratory	1 mL	EUR 346.8

Monophosphoryl Lipid A (Synthetic)
VAdv-Ly0012-1mg	Creative Biolabs	1 mg	EUR 1033.2
Description: Synthetic Monophosphoryl Lipid A, pure compound, a TLR4-based vaccine adjuvant.

Monophosphoryl Lipid A (Synthetic)
VAdv-Ly0012-5mg	Creative Biolabs	5 mg	EUR 3013.2
Description: Synthetic Monophosphoryl Lipid A, pure compound, a TLR4-based vaccine adjuvant.

Synthetic Monophosphoryl Lipid A
VAdv-Ly0031	Creative Biolabs	1 mg	EUR 2557.2
Description: Synthetic monophosphoryl lipid A, a TLR4 agonist vaccine adjuvant.

MicroGram Lipid Assay Kit
LIP1000	ProFoldin	1000 assays	EUR 263.3
Description: This product includes 0.1 ml of 1000 x LIP 10 Dye. It is for measurement of 1000 samples using 96-well plates. Cuvettes may also be used for measurements.

LipidSpot 488 Lipid Droplet Stain
70065	Biotium	125uL	EUR 481.2
Description: Minimum order quantity: 1 unit of 125uL

LipidSpot 488 Lipid Droplet Stain
70065-T	Biotium	20uL	EUR 139.2
Description: Minimum order quantity: 1 unit of 20uL

LipidSpot 610 Lipid Droplet Stain
70069	Biotium	125uL	EUR 481.2
Description: Minimum order quantity: 1 unit of 125uL

LipidSpot 610 Lipid Droplet Stain
70069-T	Biotium	20uL	EUR 136.8
Description: Minimum order quantity: 1 unit of 20uL

Serum Albumin, Lipid Free Protein
20-abx260049	Abbexa	EUR 927.60 EUR 343.20 EUR 627.60	100 mg 10 mg 50 mg

Goat Lipid peroxide ELISA kit
E06L0263-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.