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Recombinant Proteinase K (PROK), Molecular Biology Grade

Proteinase Okay (Lyophilized)

For Primary Digestion of Protein in Pure Samples

  • Energetic over a pH differ of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100
  • Purified to remove RNase and DNase actions

Safe at Room Temperature and Easy to Use

  • Energetic over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
  • Retains >80% put together at temperatures as tons as 60°C
  • No resuspension or thawing forward of use
  • Cat.# MC5005, MC5008 offered at a spotlight of 20mg/ml

Proteinase Okay – one amongst many often used enzymes in molecular biology.

Great fully totally different for protein digestion in assorted choices.

  • BLIRT – knowledgeable in Proteinase Okay manufacturing for over 15 years.
  • Licensed manufacturing course of ISO 13485 ensuing all via the very best high quality product with a extremely low batch-to-batch variability.
  • Monumental elements, the potential for white labeling, and portioning service – liquid choice all via the amount on the consumer’s request (as tons as numerous million samples month-to-month).

 

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even all via the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Recombinant Proteinase K
Recombinant Proteinase Okay


Highlights

• Ready-to-use reply
• Energetic in quite a lot of response circumstances

Capabilities

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

Keep in mind

• The actually helpful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The put together of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to 4 M urea
• Ca2+ protects Proteinase Okay in path of autolysis, will improve the thermal stability, and has a regulatory carry out for the substrate binding web page of Proteinase Okay
• Safe over a large pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum put together at 50 to55°C
• Speedy denaturation of enzyme occurs at temperatures above 65°C.

 

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad-spectrum endopeptidase with a extremely extreme explicit put together.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the best high quality product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is stuffed with life beneath quite a lot of response circumstances, along with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively using for the digestion of proteins, along with DNases and RNases, all by means of nucleic acid preparations with out compromising the integrity of isolated DNA or RNA.

Choices

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • Extreme put together and distinctive purity.
  • Energetic at extreme temperatures (as tons as 56 °C) and denaturing circumstances (e.g. all via the presence of urea and/or SDS), which makes it greatest for digesting proteins in numerous choices.
  • Safe over a large pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Accessible as powder, lyophilized “cake” or liquid.

Capabilities:

  • Extraction of DNA and RNA from totally fully fully totally different starting presents.
  • Purification of aim offers from contaminating proteins.
  • Elimination of DNases and RNases all by means of nucleic acids isolation.

 

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