A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals

A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals

Middle East respiratory syndrome (mer) is a zoonosis caused by the mer deadly coronavirus (Mers-CoV) and pose a significant threat to public health worldwide. Therefore, serology rapid, sensitive, and specific for the detection of antibodies anti-Mers-CoV in both humans and animals is indispensable for the successful management of this disease. Here, we evaluated a wide …

A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals Read More »

Detection of Antibodies Against Canine Circovirus in Naturally and Experimentally Infected Canines by Recombinant Capsid Enzyme-Linked Immunosorbent Assay

Detection of Antibodies Against Canine Circovirus in Naturally and Experimentally Infected Canines by Recombinant Capsid Enzyme-Linked Immunosorbent Assay

Canine Circovirus (CanineCV), a new pathogen, found to be associated with hemorrhagic diarrhea dog, vasculitis, granulomatous lymphadenitis, and acute gastroenteritis. Although CanineCV is a very positive level in diarrhea cases, pathogenicity remains controversial. In this study, the prevalence and risk factors associated CanineCV infection among domestic dogs in northeastern China were investigated by enzyme-linked immunosorbent …

Detection of Antibodies Against Canine Circovirus in Naturally and Experimentally Infected Canines by Recombinant Capsid Enzyme-Linked Immunosorbent Assay Read More »

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Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults

The relationship between intestinal permeability (IP) and body composition remains unclear. The gold standard test differential-absorption sugars are difficult to resolve, with the zonulin is being increasingly used as an independent biomarker IP. This pilot study aims to explore the relationship between small IP, zonulin concentration, and body composition in healthy adults. The ratio of …

Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults Read More »

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Enzyme-linked immunosorbent assays using virus-like particles containing mutations of conserved residues on envelope protein can distinguish three flavivirus infections

The recent outbreak of Zika virus (ZIKV) in areas endemic flavivirus-highlight the need for sensitive and specific serological tests. Previously, we and others reported the fusion loop lock (FL) residue and / or the BC loop (BCL) residues on the envelope protein of dengue virus (DENV) is recognized by flavivirus cross-reactive human monoclonal antibodies and …

Enzyme-linked immunosorbent assays using virus-like particles containing mutations of conserved residues on envelope protein can distinguish three flavivirus infections Read More »

A uniform quantitative enzyme-linked immunosorbent assay for Coxsackievirus A16 antigen in vaccine

A uniform quantitative enzyme-linked immunosorbent assay for Coxsackievirus A16 antigen in vaccine

Coxsackievirus A16 (CV-A16), one of the major etiological agent of hand, foot and mouth disease (HFMD), causing outbreaks of disease in young children throughout the world. In order to promote the prevention and control of HFMD, research and development of vaccines CV-A16 has been done in China. However, due to lack of CV-A16 antigen detection …

A uniform quantitative enzyme-linked immunosorbent assay for Coxsackievirus A16 antigen in vaccine Read More »